A5034474972- MicroRNA in disease regulation
- Epilepsy research and treatment
- EEG and Brain-Computer Interfaces
- Advanced biosensing and bioanalysis techniques
- RNA Interference and Gene Delivery
- Neuroscience and Neuropharmacology Research
- Bone Tissue Engineering Materials
- Neuroscience and Neural Engineering
- Cancer Cells and Metastasis
- Machine Learning in Bioinformatics
- Bone fractures and treatments
- Orthopedic Infections and Treatments
- Advanced Memory and Neural Computing
- 3D Printing in Biomedical Research
- RNA regulation and disease
- Ion channel regulation and function
- Neural dynamics and brain function
- Pain Mechanisms and Treatments
- Machine Learning and ELM
- biodegradable polymer synthesis and properties
- Neurological disorders and treatments
- Botulinum Toxin and Related Neurological Disorders
- Cancer Genomics and Diagnostics
- Functional Brain Connectivity Studies
- Circular RNAs in diseases
University of Medicine and Health Sciences
2025
Philipps University of Marburg
2016-2024
Goethe University Frankfurt
2016-2023
Royal College of Surgeons in Ireland
2019-2023
University Hospital Frankfurt
2018-2023
Current anti-epileptic drugs (AEDs) act on a limited set of neuronal targets, are ineffective in third patients with epilepsy, and do not show disease-modifying properties. MicroRNAs small noncoding RNAs that regulate levels proteins by post-transcriptional control mRNA stability translation. MicroRNA-134 is involved controlling microstructure brain excitability previous studies showed intracerebroventricular injections locked nucleic acid (LNA), cholesterol-tagged antagomirs targeting...
Treating bone infections and ensuring repair is one of the greatest global challenges modern orthopedics, made complex by antimicrobial resistance (AMR) risks due to long-term antibiotic treatment debilitating large defects following infected tissue removal. An ideal multi-faceted solution would will eradicate bacterial infection without use, simultaneously stimulating osteogenesis angiogenesis. Here, a multifunctional collagen-based scaffold that addresses these needs leveraging potential...
Temporal lobe epilepsy is the most common drug-resistant form of in adults. The reorganization neural networks and gene expression landscape underlying pathophysiologic network behavior brain structures such as hippocampus has been suggested to be controlled, part, by microRNAs. To systematically assess their significance, we sequenced Argonaute-loaded microRNAs define functionally engaged three different animal models two species at six time points between initial precipitating insult...
Epilepsy diagnosis is complex, requires a team of specialists and relies on in-depth patient family history, MRI-imaging EEG monitoring. There therefore an unmet clinical need for non-invasive, molecular-based, biomarker to either predict the development epilepsy or diagnose with who may not have had witnessed seizure. Recent studies demonstrated role microRNAs in pathogenesis epilepsy. MicroRNAs are short non-coding RNA molecules which negatively regulate gene expression, exerting profound...
There remains an urgent need for new therapies treatment-resistant epilepsy. Sodium channel blockers are effective seizure control in common forms of epilepsy, but loss sodium function underlies some genetic Approaches that provide bidirectional expression needed. MicroRNAs (miRNA) small noncoding RNAs which negatively regulate gene expression. Here we show genome-wide miRNA screening hippocampal tissue from a rat epilepsy model, mice treated with the antiseizure medicine cannabidiol, and...
Abstract Osteomyelitis, a severe bone infection, is an extremely challenging complication in the repair of traumatic defects. Furthermore, use long-term high-dose antibiotics standard treatment increases risks antibiotic resistance. Herein, antibiotic-free, collagen silver-doped hydroxyapatite (coll-AgHA) scaffold reinforced with 3D printed polycaprolactone (PCL) framework was developed enhanced mechanical properties to be used load-bearing defects antimicrobial as preventative measure...
Nonviral vectors offer a safe alternative to viral for gene therapy applications, albeit typically exhibiting lower transfection efficiencies. As result, there remains significant need the development of nonviral delivery system with low cytotoxicity and high efficacy as tool transient delivery. This study assesses MgAl-NO3 layered double hydroxide (LDH) vector deliver nucleic acids (pDNA, miRNA siRNA) mesenchymal stromal cells (MSCs) in 2D culture using 3D tissue engineering scaffold...
Abstract Epileptogenesis is the process by which a normal brain becomes hyperexcitable and capable of generating spontaneous recurrent seizures. The extensive dysregulation gene expression associated with epileptogenesis shaped, in part, microRNAs (miRNAs) – short, non-coding RNAs that negatively regulate protein levels. Functional miRNA-mediated regulation can, however, be difficult to elucidate due complexity miRNA-mRNA interactions. Here, we integrated miRNA mRNA profiles sampled over...
Kainic acid (KA) is a potent glutamate analog that used to induce neurodegeneration and model temporal lobe epilepsy (TLE) in rodents. KA reliably induces severe, prolonged seizures, is, convulsive status epilepticus (cSE), which typically fatal without pharmacologic intervention. Although the use of human has proven unquestionably valuable for >30 years, significant variability mortality continue confound results. These issues are probably consequence cSE, an all-or-nothing response...
Abstract Background Cancer pain is associated with increased sensitivity to noxious (hyperalgesia) and normally innocuous (allodynia) stimuli due activation of nociceptors by tumour‐derived mediators or tumour infiltration nerves. The sensitization accompanied modifications in gene expression, but specifically regulated genes are largely unknown. 25 kDa synaptosomal‐associated protein ( SNAP ‐25) involved chemical neurotransmission at the synaptic cleft. Its inhibition Botulinum neurotoxin A...
Epilepsy is one of the most common neurological disorders affecting patients across all ages. During progression disease, termed epileptogenesis (EPG), may not yet show any clinical manifestation. The EPG phase can range from weeks to years and with epilepsy are usually diagnosed by occurrence a spontaneous seizure followed electroencephalography (EEG) monitoring in hospital. However, more seizures they have, less effective treatment will be. Detecting development before first s eizure llow...
Inflammation, particularly cytokine release, contributes to epileptogenesis by influencing the cerebral tissue remodeling and neuronal excitability that occurs after a precipitating epileptogenic insult. While several cytokines have been explored in this process, release kinetics are less well investigated. Determining time course of zone is necessary for precisely timed preventive or therapeutic anti-inflammatory interventions.Hippocampal extracellular levels six chemokines (IL-1β, IL-6,...
Epilepsy is a common neurological disorder characterized by recurrent seizures accompanied excessive synchronous brain activity. The process of structural and functional alterations leading to increased seizure susceptibility eventually spontaneous called epileptogenesis (EPG) can span months or even years. Detecting monitoring the progression EPG could allow for targeted early interventions that slow down disease halt its development. Here, we propose an approach staging using deep neural...
Abstract MicroRNAs (miRNAs) are short noncoding RNAs that shape the gene expression landscape, including during pathogenesis of temporal lobe epilepsy (TLE). In order to provide a full catalog miRNA changes happen experimental TLE, we sequenced Argonaute 2-loaded miRNAs in hippocampus three different animal models at regular intervals between time initial precipitating insult establishment spontaneous recurrent seizures. The commonly upregulated were selected for functional vivo screen using...
Epilepsy is one of the most common neurological disorders, affecting about 1% population at all ages. Detecting development epilepsy, i.e., epileptogenesis (EPG), before any seizures occur could allow for early interventions and potentially more effective treatments. Here, we investigate if modern machine learning (ML) techniques can detect EPG from intra-cranial electroencephalography (EEG) recordings prior to occurrence seizures. For this use a rodent model epilepsy where triggered by...
Abstract There remains an urgent need for new therapies drug-resistant epilepsy (DRE). Sodium channel blockers are effective seizure control in common forms of epilepsy, but loss sodium function underlies some genetic epilepsy. Approaches that provide bi-directional expression needed. MicroRNAs (miRNA) small non-coding RNAs which negatively regulate gene expression. Here, we show genome-wide miRNA screening hippocampal tissue from a rat model, mice treated with the novel anti-seizure...
SummaryEpileptogenesis is the process by which a normal brain becomes hyperexcitable and capable of generating spontaneous recurrent seizures. The extensive dysregulation gene expression associated with epileptogenesis shaped, in part, microRNAs (miRNAs) – short, non-coding RNAs that negatively regulate protein levels. Functional miRNA-mediated regulation can, however, be difficult to elucidate due complexity miRNA-mRNA interactions. Here, we integrated miRNA mRNA profiles sampled over...
Epilepsy is a common neurological disorder characterized by recurrent seizures accompanied excessive synchronous brain activity. The process of structural and functional alterations leading to increased seizure susceptibility eventually spontaneous called epileptogenesis (EPG) can span months or even years. Detecting monitoring the progression EPG could allow for targeted early interventions that slow down disease halt its development. Here, we propose an approach staging using deep neural...