A5033733949- Polyomavirus and related diseases
- Zebrafish Biomedical Research Applications
- Developmental Biology and Gene Regulation
- Wnt/β-catenin signaling in development and cancer
- Erythrocyte Function and Pathophysiology
- Ion channel regulation and function
- Congenital heart defects research
- Cancer-related gene regulation
- Neuroinflammation and Neurodegeneration Mechanisms
- RNA and protein synthesis mechanisms
- Cancer, Hypoxia, and Metabolism
- Advanced Neuroimaging Techniques and Applications
- S100 Proteins and Annexins
- Ubiquitin and proteasome pathways
- RNA Research and Splicing
- RNA modifications and cancer
- Neurogenesis and neuroplasticity mechanisms
- Neonatal and fetal brain pathology
- Functional Brain Connectivity Studies
University of Colorado Denver
2024
University of California, San Francisco
2021-2023
University of Washington
2021-2023
Touch system function requires precise interactions between specialized skin cells and somatosensory axons, as exemplified by the vertebrate mechanosensory Merkel cell-neurite complex. Development patterning of associated neurites during organogenesis remain poorly understood, partly due to in utero development mammalian embryos. Here, we discover zebrafish epidermis identify Atonal homolog 1a (Atoh1a) a marker cells. We show that derive from basal keratinocytes, express neurosecretory...
Somatosensory neurons extend enormous peripheral axons to the skin, where they detect diverse environmental stimuli. are easily damaged due their small caliber and superficial location. Axonal damage results in Wallerian degeneration, creating vast quantities of cellular debris that phagocytes must remove maintain organ homeostasis. The mechanisms ensure efficient clearance axon from stratified adult skin unknown. Here, we established zebrafish scales as a tractable model study degeneration...
ABSTRACT Skeletal elements frequently associate with vasculature and somatosensory nerves, which regulate bone development homeostasis. However, the deep, internal location of bones in many vertebrates has limited vivo exploration neurovascular-bone relationship. Here, we use zebrafish caudal fin, an optically accessible organ formed repeating bony ray skeletal units, to determine cellular relationship between endothelium. In adult zebrafish, establish presence axons running through inside...
Abstract Neurodevelopmental mechanisms have evolved to support the formation of diverse brain structures, such as in humans, during perinatal period. Here, we demonstrate that neonatal gyrencephalic brains harbor an expanded subventricular zone, termed Arc, defined by tiered arrangement doublecortin (DCX)-expressing neuroblasts and vascular enrichment at ventricular wall. The Arc is origin dorsal ventral populations migratory target multiple regions involved higher cognitive functions....
The poly(A) signal, together with auxiliary elements, directs cleavage of a pre-mRNA and thus determines the 3' end mature transcript. In many species, including humans, signal is an AAUAAA hexamer, but we recently found that deeply branching eukaryote Giardia lamblia uses distinct hexamer (AGURAA) lacks any known elements. Our discovery prompted us to explore evolutionary dynamics signals elements in eukaryotic kingdom. We used direct RNA sequencing determine for four protists within...
The open reading frame does more than merely encode a linear peptide sequence; it is reservoir of regulatory information. Here, as part investigations into how the N-terminal amino acids regulate translation, we serendipitously uncovered new N-degron that revealed an additional layer regulation in these pathways. Using reporter assays, discovered peptides bearing position 3 arginine or lysine residues at N-terminus were rapidly degraded mammalian cells. We found this pathway requires MetAP2,...
Cadherin-associated protein p120 catenin regulates cell adhesion and migration in cultures is required for axial elongation embryos. Its roles are regulated by phosphorylation. We determined the effects of phosphorylation six serine three threonine residues during zebrafish (Danio rerio) embryogenesis.We knocked down endogenous catenin-δ1 with an antisense RNA-splice-site morpholino (Sp-MO) causing defects axis elongation. These were rescued co-injections mRNAs wildtype mouse catenin-δ1-3A...
Summary Somatosensory neurons extend enormous peripheral axons to the skin, where they detect diverse environmental stimuli. are easily damaged due their small caliber and superficial location. Axonal damage results in Wallerian degeneration, creating vast quantities of cellular debris that phagocytes must remove maintain organ homeostasis. The mechanisms ensure efficient clearance axon from stratified adult skin unknown. Here, we establish zebrafish scales as a tractable model study...
Abstract Background The cadherin‐associated protein p120 catenin regulates convergent extension through interactions with cadherin proteins, Cdc42, and Rac1, as we previously showed in zebrafish ( Danio rerio ). Phosphorylation of changes the nature its activity vitro but is virtually unexplored embryos. We used our developed antisense RNA splice‐site morpholino targeted to endogenous catenin‐δ1 cause defects axis elongation probing functions three tyrosine‐phosphorylation sites gastrulating...
ABSTRACT Touch system function requires precise interactions between specialized skin cells and somatosensory axons, as exemplified by the vertebrate mechanosensory Merkel cell-neurite complex. Development patterning of associated neurites during organogenesis remains poorly understood, partly due to in utero development mammalian embryos. Here, we discover zebrafish epidermis identify Atonal homolog 1a (Atoh1a) a marker cells. We show that derive from basal keratinocytes, express...
Abstract Skeletal elements frequently associate with vasculature and somatosensory nerves, which regulate bone development homeostasis. However, the deep, internal location of bones in many vertebrates has limited vivo exploration neurovascular-bone relationship. Here, we use zebrafish caudal fin, an optically accessible organ formed repeating bony ray skeletal units, to determine cellular relationship between bones, endothelium. In adults, establish presence axons running through inside fin...