A5000323379- Advanced biosensing and bioanalysis techniques
- CRISPR and Genetic Engineering
- SARS-CoV-2 detection and testing
- SARS-CoV-2 and COVID-19 Research
- Animal Virus Infections Studies
- RNA Interference and Gene Delivery
- Sperm and Testicular Function
- Virus-based gene therapy research
- RNA Research and Splicing
- Polymer composites and self-healing
- Biotin and Related Studies
- Sphingolipid Metabolism and Signaling
- Silicone and Siloxane Chemistry
- Flame retardant materials and properties
- CAR-T cell therapy research
- RNA regulation and disease
- Autoimmune Bullous Skin Diseases
- Monoclonal and Polyclonal Antibodies Research
- Phagocytosis and Immune Regulation
- Tryptophan and brain disorders
- Chronic Lymphocytic Leukemia Research
- RNA modifications and cancer
- Neuroinflammation and Neurodegeneration Mechanisms
- Immune Cell Function and Interaction
- Cancer Immunotherapy and Biomarkers
Case Western Reserve University
2022
Texas Tech University
2019-2022
Texas Tech University Health Sciences Center
2019-2022
University of California, Irvine
2021-2022
SUNY Downstate Health Sciences University
2019
McMaster University
2018
University at Buffalo, State University of New York
2018
We analyzed data from two ongoing COVID-19 longitudinal serological surveys in Orange County, CA., between April 2020 and March 2021. A total of 8476 finger stick blood specimens were collected before after a vaccination campaign. IgG levels determined using multiplex antigen microarray containing antigens SARS-CoV-2, SARS, MERS, Common CoV, Influenza. Twenty-six percent unvaccinated County residents December SARS-CoV-2 seropositive; out 852 seropositive individuals 77 had symptoms 9 sought...
Abstract Programmed death ligand 1 (PD-L1) plays a key role in glioblastoma multiforme (GBM) immunosuppression, vitality, proliferation, and migration, is therefore promising target for treating GBM. CRISPR/Cas9-mediated genomic editing can delete both cell surface intracellular PD-L1. This systemic deliverable PD-L1 deletion system be used as an effective anti-GBM therapy by inhibiting tumor growth overcoming immunosuppression. To CRISPR/Cas9 gene editing, we first identified two single...
Background: Parkinson's disease (PD) and multiple system atrophy (MSA) patients often suffer from gastrointestinal (GI) dysfunction GI dysbiosis (microbial imbalance).GI also occurs in mouse models of PD MSA.Objectives: To assess gut subjects as compared to controls, identify potential shared microbial taxa humans MSA, the effects therapies on microbiota.Methods: In this human pilot study, function was assessed by fecal consistency/frequency measured using Bristol Stool Form Scale transit...
Recent studies provide conflicting evidence on the persistence of SARS-CoV-2 immunity induced by mRNA vaccines. Here, we aim to quantify humoral following vaccination using a coronavirus antigen microarray that includes 10 antigens. In prospective longitudinal cohort 240 healthcare workers, composite IgG antibody levels did not wane significantly over 6-month study period. subset population previously exposed based seropositivity for nucleocapsid antibodies, higher anti-spike were measured...
Soybean oil-derived polyurethanes, while much greener than those derived from isocyanates, have a problematic combustion profile; concerns exist about the nitrogen-containing compounds found in smoke. Silicones typically burn cleanly to form an insulating silica layer upon combustion. We examined ability of silicones convey improved thermal stability soybean oil. The oil was modified via ene reaction incorporate alkoxysilane functionality that used subsequent Piers–Rubinsztajn with create...
Abstract 35S rRNA transcripts include a 5′-external transcribed spacer followed by rRNAs of the small and large ribosomal subunits. Their processing yields massive precursors that dozens assembly factor proteins. In Saccharomyces cerevisiae, nucleolar factors form 2 coaxial layers/volumes around DNA. Most these are cyclically recruited from latent state to an operative state, extensively conserved. The layers match, at least approximately, known subcompartments found in higher eukaryotic...
Delivery of plasmid DNA to transfect human primary macrophages is extremely difficult, especially for genetic engineering. Engineering imperative the treatment many diseases including infectious diseases, cancer, neurological and aging. Unfortunately, does not cross nuclear membranes terminally differentiated integrate (pDNA) into their genome. To address this issue, we have developed a core-shell nanoparticle (NP) using our newly created cationic lipid deliver anti-inflammatory cytokine...
A 53-year-old female presented with a several-month history of painless left eyelid lesion. Exam revealed circumscribed pink 0.5 × 0.4 cm superficial papule in the central upper superior to tarsus (Fig. A). Excisional biopsy disclosed lymphocyte proliferation B) that was polytypic minority staining for B-cell marker CD20 C) and majority T-cell CD3 D). Gene rearrangement also confirmed polyclonality diagnosis reactive lymphoid hyperplasia (LH). Systemic workup negative surveillance has shown...
Abstract Glioblastoma multiforme (GBM) is a devastating cancer that develops from astrocytes in the brain. GBM fast acting and kills 90% of patients within 5 years. Several immunotherapies have been developed to treat GBM, however, major challenges still persist. For example, checkpoint proteins such as programmed cell death protein 1 (PD-1) its ligand, ligand (PD-L1), are upregulated cells evade immune system. Targeting PD-L1 for genetic knockdown thus promising avenue treatment GBM....
<h3>Background</h3> Immune checkpoint therapy has achieved durable clinical responses, but only in a subset of cancer patients. Immunosuppressive myeloid cells, heterogenous group innate immune have emerged as key contributors to resistance T cell based therapy. Leukocyte immunoglobulin-like receptor B4 (LILRB4), also known transcript 3 (ILT3), is an inhibitory selectively expressed on enriched the tumor microenvironment and contributes myeloid-driven immunosuppression. Recently, fibronectin...