A5035568455- Reproductive tract infections research
- SARS-CoV-2 and COVID-19 Research
- COVID-19 Clinical Research Studies
- Immune Response and Inflammation
- Cervical Cancer and HPV Research
- SARS-CoV-2 detection and testing
- Reproductive System and Pregnancy
- Pediatric health and respiratory diseases
- Wnt/β-catenin signaling in development and cancer
- Urinary Tract Infections Management
- Hepatitis B Virus Studies
- Animal Virus Infections Studies
- Cancer Immunotherapy and Biomarkers
- Long-Term Effects of COVID-19
- Reproductive Physiology in Livestock
- Single-cell and spatial transcriptomics
- Genital Health and Disease
- COVID-19 Impact on Reproduction
- vaccines and immunoinformatics approaches
- Cancer, Stress, Anesthesia, and Immune Response
- Monoclonal and Polyclonal Antibodies Research
- COVID-19 and healthcare impacts
- Immunotherapy and Immune Responses
- Cancer Genomics and Diagnostics
- Infection Control and Ventilation
University of California, Irvine
2015-2025
UC Irvine Health
2012-2023
University of California System
2014-2019
University of California, Irvine Medical Center
2019
Irvine Valley College
2019
University of Iowa
2005-2007
University of Washington
2005
Pseudomonas aeruginosa causes chronic biofilm infections, and its ability to attach surfaces other cells is important for formation maintenance. Mutations in a gene called wspF , part of putative chemosensory signal-transduction operon, have been shown result cell aggregation altered colony morphology. The WspF phenotypes depend on the presence WspR, which member family signal transduction proteins known as response regulators. It likely that effect mutation cause constitutive activation...
Background The coronavirus disease 2019 (COVID-19) pandemic has created one of the largest global health crises in almost a century. Although current rate Severe acute respiratory syndrome 2 (SARS-CoV-2) infections decreased significantly, long-term outlook COVID-19 remains serious cause morbidity and mortality worldwide, with still substantially surpassing even that recorded for influenza viruses. continued emergence SARS-CoV-2 variants concern (VOCs), including multiple heavily mutated...
Sepsis due to antimicrobial resistant pathogens is a major health problem worldwide. The inability rapidly detect and thus treat bacteria with appropriate agents in the early stages of infections leads excess morbidity, mortality, healthcare costs. Here we report rapid diagnostic platform that integrates novel one-step blood droplet digital PCR assay high throughput 3D particle counter system potential perform bacterial identification antibiotic susceptibility profiling directly from whole...
Abstract Over the last two decades, there have been three deadly human outbreaks of coronaviruses (CoVs) caused by SARS-CoV, MERS-CoV, and SARS-CoV-2, which has current COVID-19 global pandemic. All CoVs originated from bats transmitted to humans via various intermediate animal reservoirs. It remains highly possible that other COVID pandemics will emerge in coming years yet another spillover a bat-derived SARS-like coronavirus (SL-CoV) into humans. Determining Ag B cells, CD4+ CD8+ T cell...
We analyzed data from two ongoing COVID-19 longitudinal serological surveys in Orange County, CA., between April 2020 and March 2021. A total of 8476 finger stick blood specimens were collected before after a vaccination campaign. IgG levels determined using multiplex antigen microarray containing antigens SARS-CoV-2, SARS, MERS, Common CoV, Influenza. Twenty-six percent unvaccinated County residents December SARS-CoV-2 seropositive; out 852 seropositive individuals 77 had symptoms 9 sought...
Background Cross-reactive SARS-CoV-2-specific memory CD4 + and CD8 T cells are present in up to 50% of unexposed, pre-pandemic, healthy individuals (UPPHIs). However, the characteristics cross-reactive associated with subsequent protection asymptomatic coronavirus disease 2019 (COVID-19) patients (i.e., unvaccinated who never develop any COVID-19 symptoms despite being infected SARS-CoV-2) remains be fully elucidated. Methods This study compares antigen specificity, frequency, phenotype,...
Pseudomonas aeruginosa has sets of sensory genes designated che and che2. The are required for flagella-mediated chemotaxis. che2 expressed in the stationary phase growth probably also involved behavioural responses. P. 26 chemoreceptor genes, six which preferentially phase. Subcellular localization experiments indicated that Che proteins form signal transduction complexes at cell poles throughout growth. Cyan fluorescent protein (CFP)-tagged McpA, a phase-expressed chemoreceptor, appeared...
While severe coronavirus 2019 (COVID-19) is associated with immune activation at the maternal-fetal interface, responses to asymptomatic/mild acute respiratory syndrome 2 (SARS-CoV-2) infection during pregnancy remain unknown. Here, we assess immunological adaptations in blood and term decidua response disease pregnant women. We report attenuated antigen presentation type I interferon (IFN) signaling pathways, loss of tissue-resident decidual macrophages, upregulated cytokine/chemokine...
Abstract SARS-CoV-2 variants of concern (VOCs) continue to evolve and reemerge with chronic inflammatory long COVID sequelae, necessitating the development anti-inflammatory therapeutic molecules. Therapeutic effects receptor for advanced glycation end products (RAGE) were reported in many diseases. However, a effect RAGE COVID-19 has not been reported. In present study, we investigated whether how RAGE-Ig fusion protein would have an antiviral system. The protective was determined vivo...
Studies in humans indicate that certain Chlamydia trachomatis serovars are more pathogenic than others. Specifically, several studies concluded from the C-complex those B-complex, although there reports do not support this finding. To investigate these results an animal model, eight genitourinary C. were tested two strains of mice: C3H/HeN and BALB/c. These mice investigated because is susceptible to muridarum infections BALB/c, indicative differences their immunogenetic background. Mice...
Abstract Respiratory syncytial virus (RSV) is a ubiquitous that preferentially infects airway epithelial cells, causing asthma exacerbations and severe disease in immunocompromised hosts. Acute RSV infection induces inflammation the lung. Thymus- activation-regulated chemokine (TARC) recruits Th2 cells to sites of inflammation. We found acute BALB/c mice increased TARC production Immunization with individual proteins can lead development Th1- or Th2-biased T cell responses lung after...
There is a need to implement vaccine protect against Chlamydia trachomatis infections. To test new vaccine, mice were immunized with the muridarum native major outer membrane protein (nMOMP) solubilized either amphipol A8-35 or detergent Z3-14. OVA was used as negative control, and inoculated intranasally C. positive controls. Animals vaccinated nMOMP mounted strong Chlamydia-specific humoral cell-mediated immune responses. Mice nMOMP/A8-35 had higher ratio of Abs denatured elementary bodies...
Chlamydia trachomatis is the most common sexually transmitted bacterial pathogen and etiologic agent of blinding trachoma. Intracellular signaling pathways leading to host cell inflammation innate immunity include those mediated by Toll-like receptors (TLRs) nucleotide binding oligomerization domain 1 (Nod1) protein. In epithelial cells, TLR-dependent contributes local immune responses via induction inflammatory mediators. There evidence that TLR3, TLR4, and, particularly, TLR2 are critical...
Effective methods for predicting COVID-19 disease trajectories are urgently needed. Here, enzyme-linked immunosorbent assay (ELISA) and coronavirus antigen microarray (COVAM) analysis mapped antibody epitopes in the plasma of patients (
ABSTRACT The first-generation Spike-alone-based COVID-19 vaccines have successfully contributed to reducing the risk of hospitalization, serious illness, and death caused by SARS-CoV-2 infections. However, waning immunity induced these failed prevent immune escape many variants concern (VOCs) that emerged from 2020 2024, resulting in a prolonged pandemic. We hypothesize next-generation Coronavirus (CoV) vaccine incorporating highly conserved non-Spike antigens would confer stronger broader...
The adult human breast comprises an intricate network of epithelial ducts and lobules that are embedded in connective adipose tissue. While previous studies have mainly focused on the system, many non-epithelial cell types remain understudied. Here, we constructed a comprehensive Human Breast Cell Atlas (HBCA) at single-cell spatial resolution. Our transcriptomics data profiled 535,941 cells from 62 women, 120,024 nuclei 20 identifying 11 major 53 states. These revealed abundant pericyte,...
Implementation of a vaccine is likely the best approach to curtail Chlamydia trachomatis infections. The aim this study was determine ability formulated with recombinant major outer membrane protein (MOMP) and Th1 Th2 adjuvants, delivered by combinations systemic mucosal routes, elicit long-term protection in mice against genital challenge muridarum. As negative control, were vaccinated Neisseria gonorrhoeae porinB, positive control group immunized C. muridarum live elementary bodies (EB)....