A5056277703- Cancer Cells and Metastasis
- Single-cell and spatial transcriptomics
- Cancer Genomics and Diagnostics
- Cancer, Hypoxia, and Metabolism
- Cancer Immunotherapy and Biomarkers
- Angiogenesis and VEGF in Cancer
- Pancreatic and Hepatic Oncology Research
- Immunotherapy and Immune Responses
- Cytokine Signaling Pathways and Interactions
- Liver physiology and pathology
- Nanoplatforms for cancer theranostics
- PARP inhibition in cancer therapy
- Gut microbiota and health
- Renal and related cancers
- interferon and immune responses
- Cellular Mechanics and Interactions
- Epigenetics and DNA Methylation
- Genomics, phytochemicals, and oxidative stress
- Chemokine receptors and signaling
- Molecular Biology Techniques and Applications
- Cell Image Analysis Techniques
- Pancreatic function and diabetes
- Bone and Dental Protein Studies
- Melanoma and MAPK Pathways
- Histone Deacetylase Inhibitors Research
University of California, Irvine
2021-2024
Heidelberg University
2015-2022
German Cancer Research Center
2015-2022
Heidelberg Institute for Stem Cell Technology and Experimental Medicine
2015-2022
National University Cancer Institute, Singapore
2019
National University of Singapore
2019
Abstract Metastatic colonization relies on interactions between disseminated cancer cells and the microenvironment in secondary organs. Here, we show that breast evoke phenotypic changes lung fibroblasts, forming a supportive metastatic niche. Colonization of lungs confers an inflammatory phenotype metastasis-associated fibroblasts. Specifically, IL-1α IL-1β secreted by induce CXCL9 CXCL10 production fibroblasts via NF-κB signaling, fueling growth metastases. Notably, find chemokine receptor...
Abstract Disseminated cancer cells frequently lodge near vasculature in secondary organs. However, our understanding of the cellular crosstalk invoked at perivascular sites is still rudimentary. Here, we identify intercellular machinery governing formation a pro-metastatic vascular niche during breast colonization lung. We show that specific secreted factors, induced metastasis-associated endothelial (ECs), promote metastasis mice by enhancing stem cell properties and viability cells....
Research Article6 September 2018Open Access Source DataTransparent process Stress signaling in breast cancer cells induces matrix components that promote chemoresistant metastasis Jacob Insua-Rodríguez Heidelberg Institute for Stem Cell Technology and Experimental Medicine (HI-STEM gGmbH), Heidelberg, Germany Division of Cells Cancer, German Cancer Center (DKFZ), Faculty Biosciences, University Search more papers by this author Maren Pein Tsunaki Hongu Department Physiological Chemistry...
Pancreatic ductal adenocarcinoma (PDAC) is often diagnosed when liver metastases already emerged. This study elucidated the impact of hepatic stromal cells on growth behavior premalignant and malignant pancreatic epithelial (PDECs). Liver sections tumor-bearing KPC mice comprised micrometastases displaying low proliferation located in an unobtrusive microenvironment whereas macrometastases containing more proliferating were surrounded by myofibroblasts (HMFs). In age-related syngeneic PDAC...
Abstract LNK (SH2B3) is a key negative regulator of JAK-STAT signaling which has been extensively studied in malignant hematopoietic diseases. We found that significantly elevated cutaneous melanoma; this elevation correlated with hyperactive the RAS-RAF-MEK pathway. Elevated enhances cell growth and survival adverse conditions. Forced expression inhibits by interferon-STAT1 suppresses interferon (IFN) induced cycle arrest apoptosis. In contrast, silencing either shRNA or CRISPR-Cas9...
The adult human breast comprises an intricate network of epithelial ducts and lobules that are embedded in connective adipose tissue. While previous studies have mainly focused on the system, many non-epithelial cell types remain understudied. Here, we constructed a comprehensive Human Breast Cell Atlas (HBCA) at single-cell spatial resolution. Our transcriptomics data profiled 535,941 cells from 62 women, 120,024 nuclei 20 identifying 11 major 53 states. These revealed abundant pericyte,...
<h3>Background</h3> Breast cancer associated gene 1 (BRCA1) mutant malignancy is an aggressive subtype of breast caused by mutations in the BRCA1 gene. By helping with DNA repair, plays a critical role maintaining stability cell's genetic information. Non-repaired leads to instability increased risk malignancy. Disease was thought be accumulation damage, but more recently we have evidence showing that there are other factors microenvironment such as immune cells involved initiation this...
ABSTRACT Metastatic colonization relies on interactions between disseminated cancer cells and the microenvironment in secondary organs. Here, we show that breast evoke major phenotypic changes lung fibroblasts to form a metastatic niche supports malignant growth. Colonization of lungs by confers an inflammatory phenotype associated fibroblasts, where IL-1α IL-1β, secreted cells, induce CXCL9 CXCL10 production metastasis-associated via NF-κB signaling. These paracrine fuel growth metastases....
Introduction Cancer progression to metastasis remains a major clinical challenge and is commonly associated with resistance prevailing therapies such as chemotherapy. Exposure cytotoxic may cause extensive stress responses in cancer cells. The c-Jun N-terminal kinase (JNK) pathway induced by can be activated However, the cellular outcome of JNK signalling metastatic still paradoxical poorly understood. Material methods In our experiments, we utilised 3D culture systems primary breast cells,...
Summary Women with germline mutations in BRCA1 (BRCA1 +/mut ) have increased risk for developing hereditary breast cancer 1, 2 . Cancer initiation is associated pre-malignant changes the epithelium including altered differentiation 3–5 , proliferative stress 6 and genomic instability 7 However, role of epithelium- stromal niche during BRCA1-driven tumor remains unclear. Here, we show that promotes epithelial proliferation BRCA1- driven trans Using single-cell RNAseq (scRNAseq) analysis human...
Abstract The role of Runt-domain related transcription factor 3 (Runx3) in the early development T cells is well established, however recent work has implicated Runx3 as a driver residency barrier tissues such lung and intestinal epithelium, tumor infiltrating lymphocytes murine human melanomas. We have identified subpopulation resident healthy breast exhibiting constitutive expression Runx3. Employing single-cell RNA sequencing paired with concurrent cell receptors (TCRseq, 10× Genomics)...
ABSTRACT When cancers progress to metastasis, disseminated cancer cells frequently lodge near vasculature in secondary organs. However, our understanding of the cellular crosstalk evoked at perivascular sites is still rudimentary. In this study, we identify intercellular machinery governing formation a pro-metastatic vascular niche during breast colonization lungs. We show that four secreted factors, INHBB, SCGB3A1, OPG and LAMA1, induced metastasis-associated endothelial (ECs), are...
Single cell RNA-Seq of human breast tissue requires dissociation into viable single suspensions. Here, we describe a protocol for digestion and compare two methods to obtain suspensions using either fluorescence activated sorting (FACS) or several wash steps Flowmi filtering.