A5102713900- Biosensors and Analytical Detection
- Microfluidic and Capillary Electrophoresis Applications
- Oral Health Pathology and Treatment
- Bacterial Identification and Susceptibility Testing
- Microfluidic and Bio-sensing Technologies
- Molecular Biology Techniques and Applications
- SARS-CoV-2 and COVID-19 Research
- SARS-CoV-2 detection and testing
- Head and Neck Cancer Studies
- Salivary Gland Tumors Diagnosis and Treatment
- Advanced Biosensing Techniques and Applications
- Single-cell and spatial transcriptomics
- Vascular Procedures and Complications
- Plant Pathogens and Resistance
- Innovative Microfluidic and Catalytic Techniques Innovation
- Cancer Genomics and Diagnostics
- Cell Image Analysis Techniques
- Clostridium difficile and Clostridium perfringens research
- Electrowetting and Microfluidic Technologies
- Respiratory viral infections research
- Fungal Infections and Studies
- Advanced biosensing and bioanalysis techniques
- Diagnosis and Treatment of Venous Diseases
- Venous Thromboembolism Diagnosis and Management
- Urinary Tract Infections Management
Rice University
2015-2019
Irvine Valley College
2019
New York University
2018
SensoDx (United States)
2017
California Polytechnic State University
2009
Sepsis due to antimicrobial resistant pathogens is a major health problem worldwide. The inability rapidly detect and thus treat bacteria with appropriate agents in the early stages of infections leads excess morbidity, mortality, healthcare costs. Here we report rapid diagnostic platform that integrates novel one-step blood droplet digital PCR assay high throughput 3D particle counter system potential perform bacterial identification antibiotic susceptibility profiling directly from whole...
Current cancer detection systems lack the required sensitivity to reliably detect minimal residual disease (MRD) and recurrence at earliest stages when treatment would be most effective. To address this issue, we present a novel liquid biopsy approach that utilizes an integrated comprehensive droplet digital (IC3D) PCR system which combines microfluidic partitioning, fluorescent multiplex chemistry, our rapid 3D, large-volume counting technology. The IC3D ddPCR assay can cancer-specific,...
To detect the presence of antibodies in blood against SARS-CoV-2 a highly sensitive and specific manner, here we describe robust, inexpensive ($200), 3D-printable portable imaging platform (TinyArray imager) that can be deployed immediately areas with minimal infrastructure to read coronavirus antigen microarrays (CoVAMs) contain panel antigens from SARS-CoV-2, SARS-1, MERS, other respiratory viruses. Application includes basic laboratories makeshift field clinics where few drops finger...
Introduction. The alarming rise in urinary tract infection (UTI) antimicrobial resistance has resulted from a combination of high prevalence, low specificity and the lack rapid, point-of-care (POC) antibiotic susceptibility test (AST), which led to overuse/inappropriate use antibiotics.Aim. This study aimed evaluate performance rapid POC phenotypic AST device reporting information within 2 h.Methodology. Instrument calibration was performed with model bacteria fluorescent microbeads...
High-throughput and rapid screening testing is highly desirable to effectively combat the rapidly evolving COVID-19 pandemic co-presents with influenza seasonal common cold epidemics. Here, we present a general workflow for iterative development validation of an antibody-based microarray assay detection respiratory viral panel: (a) antibody quickly identify optimal reagents conditions, (b) immunofluorescence design including signal amplification low titers, (c) characterization recombinant...
Fanconi anemia (FA) is a hereditary genomic instability disorder with predisposition to leukemia and oral squamous cell carcinomas (OSCCs). Hematopoietic stem transplantation (HSCT) facilitates cure of bone marrow failure thus extends life expectancy in FA patients; however, survival hematologic malignancies increases the risk OSCC these patients. We developed "cytology-on-a-chip" (COC)–based brush biopsy assay for monitoring patients potentially malignant disorders (OPMDs). Using this COC...
Fanconi anemia (FA) is caused by mutations of DNA repair genes. The risk oral squamous cell carcinoma (OSCC) among FA patients 800-folds higher than in the general population. Early detection OSCC, preferably at it precursor stage, critical to improve their survival. In an ongoing clinical trial, we are evaluating effectiveness programmable bio-nanochip (p-BNC)-based cytology test diagnosing potentially malignant disorders (OPMD) non-FA patients. We used this compare cytomorphometric and...
Abstract Rapidly isolating rare targets from larger, clinically relevant fluid volumes remains an unresolved problem in biomedicine and diagnosis. Here, we describe how 3D particle sorting can enrich at ultralow concentrations over 100-fold within minutes not possible with conventional approaches. Current clinical devices based on biochemical extraction microfluidic solutions typically require high and/or only process sub-milliliter time. In a proof-of-concept application, isolated bacteria...
Fluorescence-based single particle counting devices have become very powerful tools for human health-related applications such as the detection of blood-borne pathogens.Instead passing sample fluid through a thin tube or microfluidic chip, it is commonly practiced in flow cytometers and sorter devices, counters scan volume by rotation translation container.Hence, are not limited friction can large short timeframe while maintaining high sensitivity.A be detected milliliter within minutes,...
Abstract To detect the presence of antibodies in blood against SARS-CoV-2 a highly sensitive and specific manner, here we describe robust, inexpensive ($200), 3D-printable portable imaging platform (TinyArray imager) that can be deployed immediately areas with minimal infrastructure to read coronavirus antigen microarrays (CoVAMs) contain panel antigens from SARS-CoV-2, SARS-1, MERS, other respiratory viruses. Application includes basic laboratories makeshift field clinics where few drops...
Abstract Fanconi Anemia (FA) is an autosomal recessive disorder caused by mutations of DNA repair genes. The risk oral cancer (OC) among FA patients 800-times higher than in the general population, occurring at younger ages. Hematopoietic stem cell transplantation (HSCT), which greatly extends life-expectancy further increases OC these patients. Patients with cannot tolerate chemo-and radiation therapy; hence, early detection critical to improve survival. Objective: To investigate role a...