A5103143548- Cardiac Valve Diseases and Treatments
- Atrial Fibrillation Management and Outcomes
- Cardiac Arrhythmias and Treatments
- Cancer-related Molecular Pathways
- RNA modifications and cancer
- Cancer Research and Treatments
- Cardiovascular Function and Risk Factors
- Infective Endocarditis Diagnosis and Management
- Pulmonary Hypertension Research and Treatments
- Cancer, Hypoxia, and Metabolism
- Epigenetics and DNA Methylation
- Aortic Disease and Treatment Approaches
- Folate and B Vitamins Research
- Cardiac pacing and defibrillation studies
- Bacterial Genetics and Biotechnology
- RNA Research and Splicing
- Pluripotent Stem Cells Research
- Gene Regulatory Network Analysis
- Cardiovascular and Diving-Related Complications
- RNA and protein synthesis mechanisms
- Invertebrate Immune Response Mechanisms
- Cardiac electrophysiology and arrhythmias
- Polyamine Metabolism and Applications
- Crustacean biology and ecology
- Neurobiology and Insect Physiology Research
Sun Yat-sen University
2023-2025
Fudan University
2023-2025
University of California, Irvine
2012-2025
Zhongshan Hospital
2023-2025
The First Affiliated Hospital, Sun Yat-sen University
2023-2025
National Clinical Research Center for Digestive Diseases
2023-2025
XinHua Hospital
2021-2024
Shanghai Jiao Tong University
2011-2024
Shanghai Institute of Hematology
2024
Tianjin Agricultural University
2021-2022
The tumour suppressor p53 is the most frequently mutated gene in human cancer. Reactivation of mutant by small molecules an exciting potential cancer therapy. Although several compounds restore wild-type function to p53, their binding sites and mechanisms action are elusive. Here computational methods identify a transiently open pocket between loop L1 sheet S3 core domain. Mutation residue Cys124, located at centre pocket, abolishes reactivation R175H PRIMA-1, known compound. Ensemble-based...
Many cancer cells depend on exogenous methionine for proliferation, whereas non-tumorigenic can divide in media supplemented with the metabolic precursor homocysteine. This phenomenon is known as dependence of or addiction. The underlying mechanisms driving this cancer-specific addiction are poorly understood. Here we find that associated severe dysregulation pre-mRNA splicing. We used triple-negative breast and their methionine-independent derivatives R8 to compare RNA expression profiles...
Background: Atrial fibrillation (AF) has been identified as a risk factor for functional tricuspid regurgitation (FTR) in the absence of other known etiologies, although limited interventional options are available. K-Clip™, novel transcatheter annuloplasty device, is clip-based annular plication approach FTR. To date, no studies have investigated short-term outcomes K-Clip™ patients with severe FTR associated AF. Therefore, aim this study was to explore feasibility and effectiveness repair...
Background: Transcatheter aortic valve replacement (TAVR) has emerged as the recommended treatment for older patients with pure severe regurgitation (PSAR). However, efficacy of TAVR in younger (AR) remains unclear. This study explored therapeutic and safety these two groups compared their outcomes. Methods: Patients AR who underwent at Zhongshan Hospital between June 2021 September 2023 were included categorized into younger- age based on whether they ≥75 years old. The baseline...
The primary methyl group donor S-adenosylmethionine (SAM) is important for a plethora of cellular pathways including methylation nucleic acids, proteins, and the 5′ cap structure mRNAs, as well biosynthesis phospholipids polyamines. In addition, co-factor chromatin methylation, SAM an metabolite connected to establishment maintenance epigenetic marks. Here we demonstrate that cells halt cell proliferation when levels become low. Cell cycle arrest occurs primarily in G1 phase accompanied by...
The design principle of establishing an intracellular protein gradient for asymmetric cell division is a long-standing fundamental question. While the major molecular players and their interactions have been elucidated via genetic approaches, diversity redundancy natural systems complicate extraction critical underlying features. Here, we take synthetic biology approach to construct asymmetry in Escherichia coli, which normally symmetric. We demonstrate that oligomeric PopZ from Caulobacter...
Methionine and homocysteine are metabolites in the transmethylation pathway leading to synthesis of methyl-donor S-adenosylmethionine (SAM). Most cancer cells stop proliferating during methionine stress conditions, when is replaced growth media by its immediate metabolic precursor (Met-Hcy+). Non-transformed proliferate Met-Hcy+ media, making requirement an attractive target for therapy, yet there relatively little known about molecular mechanisms governing response cells. To study this...
CRP2 (cysteine-rich protein) is a vascular smooth muscle cell (VSMC)-expressed LIM-only protein. associates with the actin cytoskeleton and interacts transcription factors in nucleus to mediate gene expression. Using Csrp2 (gene symbol of mouse gene)-deficient mice, we previously demonstrated that an absence enhances VSMC migration increases neointima formation following arterial injury. Despite its importance injury, molecular mechanisms controlling expression are largely unknown....
Work in yeast models has benefitted tremendously from the insertion of epitope or fluorescence tags at native gene locus to study protein function and behavior under physiological conditions. In contrast, work mammalian cells largely relies on overexpression tagged proteins because high-quality antibodies are only available for a fraction proteome. CRISPR/Cas9-mediated genome editing recently emerged as powerful genome-modifying tool that can also be exploited insert various fluorophores...
Methionine (Met) is an essential amino acid and critical precursor to the cellular methyl donor S-adenosylmethionine. Unlike nontransformed cells, cancer cells have a unique metabolic requirement for Met are unable proliferate in growth media where replaced with its precursor, homocysteine. This vulnerability common among regardless of tissue origin known as "methionine dependence", stress sensitivity", or Hoffman effect. The response lipids stress, however, not well-understood. Using mass...
Onco-cardiology is an emerging field linking cancer with cardiovascular injury. Understanding the mechanism of cardiac injury helps improve quality life survivors. A series studies on adverse reactions to or oncological treatments has indicated that events related may occur over a longer period survival, and even years after therapy concluded. Current therapies, including chemotherapy, radiotherapy, targeted therapy, immunotherapy, have been found latent toxicity. These toxic effects are...
Several studies have shown that excessive protein degradation is a major cause of skeletal muscle atrophy induced by sepsis, and autophagy the main pathway participating in degradation. However, role sepsis still controversial. Previously, we found neuregulin-1β (NRG-1β) alleviated sepsis-induced diaphragm through phosphatidylinositol-3 kinase signaling pathway. Akt/mechanistic target rapamycin (mTOR) classic to regulate autophagy, which maintains intracellular homeostasis. This study aimed...
The application of transcatheter aortic valve replacement (TAVR) for severe pure native regurgitation (PNAR) remains limited. TAVR PNAR using an off-label device is associated with acceptable procedural success but increased early mortality. For performing patients, appropriate anatomy, especially a relatively small annulus, necessary condition successful procedure, and large annulus generally excluded. Here, we report new “sandwich” valve-in-valve implantation technique commercially...