A5047469803- Gut microbiota and health
- Clostridium difficile and Clostridium perfringens research
- Gastrointestinal motility and disorders
- Burkholderia infections and melioidosis
- Bacteriophages and microbial interactions
- Genomics and Phylogenetic Studies
- Metabolism and Genetic Disorders
- Helicobacter pylori-related gastroenterology studies
- High Altitude and Hypoxia
- Alcoholism and Thiamine Deficiency
- Microscopic Colitis
- Antibiotic Use and Resistance
- Bacterial Identification and Susceptibility Testing
- Respiratory viral infections research
- Bone Metabolism and Diseases
- Influenza Virus Research Studies
- Cell Adhesion Molecules Research
- Inflammation biomarkers and pathways
- Protease and Inhibitor Mechanisms
- Diet and metabolism studies
- Heme Oxygenase-1 and Carbon Monoxide
- S100 Proteins and Annexins
- Dermatology and Skin Diseases
- Advanced battery technologies research
- Pneumonia and Respiratory Infections
Amsterdam University Medical Centers
2018-2021
Vrije Universiteit Amsterdam
2021
University of Amsterdam
1988-2020
Amsterdam UMC Location University of Amsterdam
2009-2020
Jeroen Bosch Ziekenhuis
2019
Institute of Infection and Immunity
2014
<h3>Objective</h3> Pneumonia accounts for more deaths than any other infectious disease worldwide. The intestinal microbiota supports local mucosal immunity and is increasingly recognised as an important modulator of the systemic immune system. precise role gut in bacterial pneumonia, however, unknown. Here, we investigate function host defence against <i>Streptococcus pneumoniae</i> infections. <h3>Design</h3> We depleted C57BL/6 mice subsequently infected them intranasally with <i>S....
The intestinal microbiota has emerged as a virtual organ with essential functions in human physiology. Antibiotic-induced disruption of the critically ill patients may have negative influence on key energy resources and immunity. We set out to characterize fecal composition both without sepsis explore use microbiota-derived markers for clinical outcome measurements this setting. In prospective observational cohort study we analyzed 34 admitted intensive care unit. Fifteen healthy subjects...
The impact of combination antibiotic therapy on the composition intestinal microbiota remains ill-defined. We aimed to assess effect a 1 week regimen healthy humans for period up 31 months. Thirteen adult men received either no treatment or oral broad-spectrum antibiotics (ciprofloxacin, vancomycin and metronidazole) 7 days. At four timepoints (prior treatment, day 9, 49 8–31 months later) faecal samples were collected analysed using 16S RNA gene sequencing. short-term gut was profound, with...
Peptidylarginine deiminase 4 (PAD4) catalyzes citrullination of histones, an important step for neutrophil extracellular trap (NET) formation. We aimed to determine the role PAD4 during pneumonia. Markers NET formation were measured in lavage fluid from airways critically ill patients. and host defense studied pneumonia-derived sepsis caused by Klebsiella pneumoniae PAD4+/+ PAD4-/- mice. Patients with pneumosepsis, compared those nonpulmonary disease, showed increased citrullinated histone 3...
Bacterial microbiota play a critical role in mediating local and systemic immunity, shifts these microbial communities have been linked to impaired outcomes illness. Emerging data indicate that other intestinal organisms, including bacteriophages, viruses of eukaryotes, fungi, protozoa, are closely interlinked with the bacterial their host, yet collective during antibiotic perturbation illness remains be elucidated. We employed multi-omics factor analysis (MOFA) systematically integrate (16S...
<h3>Objective</h3> The gut microbiota is essential for the development of intestinal immune system. Animal models have suggested that also acts as a major modulator systemic innate immunity during sepsis. Microbiota disruption by broad-spectrum antibiotics could thus adverse effects on cellular responsiveness towards invading pathogens. As such, use may attribute to immunosuppression seen in We aimed test whether affects responses endotoxemia healthy subjects. <h3>Design</h3> In this...
Background Melioidosis, caused by the Gram-negative bacterium Burkholderia pseudomallei, is an emerging cause of pneumonia-derived sepsis in tropics. The gut microbiota supports local mucosal immunity and increasingly recognized as a protective mediator host defenses against systemic infection. Here, we aimed to characterize composition function intestinal during experimental melioidosis. Methodology/Principal findings C57BL/6 mice were infected intranasally with B. pseudomallei sacrificed...
Background An increasing body of evidence is indicating that the gut microbiota modulates pulmonary inflammatory responses. This so-called gut–lung axis might be importance in a whole spectrum diseases such as acute respiratory distress syndrome, chronic obstructive disease and pneumonia. Here, we investigate effect antibiotic disruption on immune responses lung after intranasal challenge with lipopolysaccharide (LPS). Methods/results C57Bl/6 mice were treated for two weeks broad-spectrum...
Abstract Patients that suffer from sepsis exhibit an early hyper-inflammatory immune response which can lead to organ failure and death. In our study, we assessed the modulation in human vivo endotoxemia model compared it ex LPS stimulation using 38 transcriptomic markers. Blood was collected before after 4 hours of challenge tested with Immune Profiling Panel (IPP) FilmArray system. The use IPP showed markers innate immunity dominated , mainly related monocytes neutrophils. Comparing two...
OBJECTIVES: Broad-spectrum antibiotics disrupt the intestinal microbiota. The microbiota is essential for physiological processes, such as development of gut immune system. Recent murine data suggest that also modulates systemic innate responses; however, evidence in humans lacking. METHODS: Twelve healthy young men were given oral broad-spectrum (ciprofloxacin 500 mg bid, vancomycin tid and metronidazole tid) 7 days. At baseline, 1 day, 6 weeks after antibiotics, blood feces sampled. Whole...
Background Triggering receptor expressed on myeloid cells (TREM) -1 and TREM-2 are key regulators of the inflammatory response that involved in clearance invading pathogens. Melioidosis, caused by "Tier 1" biothreat agent Burkholderia pseudomallei, is a common form community-acquired sepsis Southeast-Asia. TREM-1 has been suggested as biomarker for melioidosis. We aimed to characterize expression function Methodology/Principal Findings Wild-type, TREM-1/3 (Trem-1/3-/-) (Trem-2-/-) deficient...
Background: Melioidosis, caused by the gram-negative bacterium Burkholderia pseudomallei, is a common cause of community-acquired sepsis in Southeast Asia and Northern Australia. The NLRP3 inflammasome its downstream product interleukin-1 beta (IL-1β) have been proposed to play crucial roles melioidosis. In this study, we characterized role IL-1β more closely assessed therapeutic potential. Methods: mRNA expression components was determined isolated leukocytes 32 healthy controls 34 patients...
Melioidosis is a severe infectious disease with high mortality that endemic in South-East Asia and Northern Australia. The causative pathogen, Burkholderia pseudomallei, listed as potential bioterror weapon due to its virulence for easy dissemination. Currently, there no licensed vaccine prevention of melioidosis. Here, we explore the use rapid plasmid DNA vaccination against B. pseudomallei flagellin protection respiratory challenge. We tested three vaccines different subcellular targeting...
The Gram-negative intracellular pathogen Burkholderia pseudomallei is the causative agent of melioidosis, an important cause sepsis in Southeast Asia. Recognition pathogen-associated molecular patterns by Toll-like receptors (TLRs) essential for appropriate immune response during invasion. In patients with TLR5 most abundantly expressed TLR, and a hypofunctional variant has been associated improved survival. Here, we studied functional role its ligand flagellin experimental melioidosis....
A proportion of patients suspected Clostridium difficile infection are unnecessarily placed in contact isolation. By introducing a random-access glutamate dehydrogenase (GDH) test for C. difficile, we aimed to reduce isolation time. In addition, investigated whether the result toxin A&B enzyme immunoassay (EIA) was associated with decision initiate antibiotic treatment against difficile. This retrospective pre- and post-implementation study from June 3, 2016, 4, 2018. Pre-implementation,...
The human gut microbiota, formerly known as 'gut flora', may be regarded an external organ with many physiological functions in metabolism, development of the immune system and defense against pathogens. adult microbiota consist 1013-1014 micro-organisms. aggregate genome these, microbiome, is 100 times larger than genome. microbiotica involved pathogenesis a range syndromes, such inflammatory bowel disease, obesity, diabetes mellitus atopic disorders. It should noted that until now most...
Abstract Bacterial microbiota play a critical role in mediating local and systemic immunity, shifts these microbial communities have been linked to impaired outcomes illness. Emerging data indicate that other intestinal organisms, including bacteriophages, viruses of eukaryotes, fungi, protozoa, are closely interlinked with the bacterial their host, yet collective during antibiotic perturbation illness remains be elucidated. Here, multi-omics factor analysis (MOFA), novel computational...