A5008727017- Advanced biosensing and bioanalysis techniques
- Biosensors and Analytical Detection
- RNA Interference and Gene Delivery
- DNA and Nucleic Acid Chemistry
- Molecular Sensors and Ion Detection
- Nanoplatforms for cancer theranostics
- Luminescence and Fluorescent Materials
- RNA and protein synthesis mechanisms
- Supramolecular Self-Assembly in Materials
- CRISPR and Genetic Engineering
- Crystallization and Solubility Studies
- Advanced Nanomaterials in Catalysis
- Nanoparticle-Based Drug Delivery
- bioluminescence and chemiluminescence research
- Gold and Silver Nanoparticles Synthesis and Applications
- X-ray Diffraction in Crystallography
- Glycosylation and Glycoproteins Research
- Fluorine in Organic Chemistry
- Advanced Fluorescence Microscopy Techniques
- Extracellular vesicles in disease
- Mycotoxins in Agriculture and Food
- Modular Robots and Swarm Intelligence
- Virus-based gene therapy research
- Nanopore and Nanochannel Transport Studies
- Electrochemical Analysis and Applications
Hunan University
2016-2025
University of Illinois Urbana-Champaign
2018-2023
State Key Laboratory of Chemobiosensing and Chemometrics
2015-2023
Qingdao Institute of Bioenergy and Bioprocess Technology
2022
Chinese Academy of Sciences
2022
Qinghai New Energy (China)
2022
Chemo (France)
2020
Changsha University
2015-2019
Spatial and temporal distributions of metal ions in vitro vivo are crucial our understanding the roles biological systems, yet there is a very limited number methods to probe with high space time resolution, especially vivo. To overcome this limitation, we report Zn2+-specific near-infrared (NIR) DNAzyme nanoprobe for real-time ion tracking spatiotemporal control early embryos larvae zebrafish. By conjugating photocaged DNAzymes onto lanthanide-doped upconversion nanoparticles (UCNPs), have...
Abstract The quality and application of super-resolution fluorescence imaging greatly lie in the dyes’ properties, including photostability, brightness, Stokes shift. Here we report a synergistic strategy to simultaneously improve such properties regular fluorophores. Introduction quinoxaline motif with fine-tuned electron density conventional rhodamines generates new dyes vibration structure inhibited twisted-intramolecular-charge-transfer (TICT) formation synchronously, thus increasing...
Significance Long-term in situ imaging of biomolecules on the cell membrane was significant for studying physiological processes emitted from signaling and adhesion. Thus, to develop an efficient tool realize membrane-associated biomolecules, we herein proposed a de novo strategy construct antidiffusion probe by integrating fluorochrome (HYPQ) characterized strong hydrophobicity low lipophilicity, with enzyme substrate. Then, benefiting property constructed HYPQG, long-term GGT successfully...
Aptamer-based sensors have emerged as a major platform for detecting small-molecular targets, because aptamers can be selected to bind these small molecules with higher affinity and selectivity than other receptors such antibodies. However, portable, accurate, sensitive, affordable detection of targets remains challenge. In this work, we developed an aptasensing incorporating magnetic beads DNAzyme signal amplification, resulting in high sensitivity. The biosensing was constructed by...
The accurate, rapid, and sensitive identification of cancer cells in complex physiological environments is significant biological studies, personalized medicine, biomedical engineering. Inspired by the naturally confined enzymes on fluid cell membranes, a fluidly CRISPR-based DNA reporter (FINDER) was developed living which successfully applied for rapid clinical blood samples. Benefiting from spatial confinement effect improved local concentration, membrane fluidity higher collision...
RNA-cleaving DNAzymes have been demonstrated as a promising platform for sensing metal ions. However, the poor biological imaging performance of DNAzyme-based fluorescent probes has limited their intracellular applications. Compared with traditional one-photon fluorescence imaging, two-photon (TP) shown advantages such increased penetration depth, lower tissue autofluorescence, and reduced photodamage. Herein, first time, we developed an TP probe (TP-8-17ES-AuNP) Zn2+ detection in living...
A FRET-based ratiometric two-photon fluorescent probe for nitroxyl was first proposed and successfully applied in living cell tissue imaging.
Genetically encoded fluorescent proteins (FPs) have been used for metal ion detection. However, their applications are restricted to a limited number of ions owing the lack available metal-binding or peptides that can be fused FPs and difficulty in transforming binding into change signal. We report herein use Mg2+ -specific 10-23 Zn2+ 8-17 RNA-cleaving DNAzymes regulate expression as new class ratiometric sensors ions. Specifically, we demonstrate suppress Clover2, variant green FP (GFP), by...
A two photon-excited fluorescent supramolecular nanoplatform is first designed for quantitative analysis with host molecules, sensing probes and an internal reference.
Abstract Nucleic acid biosensors, integrated with functional nucleic acids, have drawn extensive attention for diverse biomedical applications. In particular, many probes been developed biosensing and imaging of tumor‐associated species, including pH, small molecules, RNAs, metal ions, proteins, cells. Despite the progress made, these sensors are mainly performed at level test tube assays or live cells disease diagnosis pathological studies. recent years, increasing research works pushing...
DNA-based probes have gained significant attention as versatile tools for biochemical analysis, benefiting from their programmability and biocompatibility. However, most existing rely on fluorescence the signal output, which can be problematic due to issues like autofluorescence scattering when applied in complex biological materials such living cells or tissues. Herein, we report development of bioluminescent nucleic acid (bioLUNA) sensors that offer laser excitation-independent ratiometric...
Multiple receptor analysis-based DNA molecular computation has been developed to mitigate the off-target effect caused by nonspecific expression of cell membrane receptors. However, it is quite difficult involve nanobodies into with programmed recognition order because "always-on" response mode and inconvenient programming. Here we propose a spatial segregation-based computing strategy shielded internal layer termed nano-phage (DNP) program nanobody build series kinetic models elucidate...
A membrane-anchored fluorescent probe for detecting K<sup>+</sup> in the cell microenvironment.
Fluorescent probes have emerged as powerful tools for the detection of different analytes by virtue structural tenability. However, requirement an excitation source largely hinders their applicability in point-of-care detection, well causing autofluorescence interference complex samples. Herein, based on bioluminescence resonance energy transfer (BRET), we developed a reaction-based ratiometric bioluminescent platform, which allows excitation-free analytes. The platform has modular design...
Plasma membranes are the fundamental mediators through which cells communicate with their surrounding environment. The techniques to monitor or synthetically manipulate cell attractive tools engineer functions of as well local microenvironment. Current advances biomolecular science enable insertion functional compounds onto cell-surface via external integration genetic engineering membrane function. Recently, DNA nanotechnology made it possible use synthetic an emerging and promising...
We report a hybridization chain reaction-based nanoprobe for selective and sensitive cancer cell recognition amplified photodynamic therapy.
Biomineralized nanoparticles enable an enzyme-assisted DNA signal amplification in living cells for microRNA imaging.
A series of physiological barriers have impeded nanoparticle-based drug formulations (NDFs) from reaching their targeted sites and achieving therapeutic outcomes. In this study, we develop size-controllable stealth doxorubicin-loaded nanodrug coated with CD47 peptides (DOX/sNDF-CD47) based on supramolecular chemistry to overcome multiple biological barriers. The smart DOX/sNDF-CD47 can efficiently decrease sequestration by macrophages disassemble into poly(amidoamine) dendrimers nuclear...