A5027795182- Aquatic Ecosystems and Phytoplankton Dynamics
- Marine and coastal ecosystems
- Microbial Community Ecology and Physiology
- Biocrusts and Microbial Ecology
- Geochemistry and Elemental Analysis
- Forest Ecology and Biodiversity Studies
- Mycorrhizal Fungi and Plant Interactions
- Wastewater Treatment and Nitrogen Removal
- Isotope Analysis in Ecology
- Radioactive element chemistry and processing
- Mine drainage and remediation techniques
- Wildlife Ecology and Conservation
- Ecology and Vegetation Dynamics Studies
- Chromium effects and bioremediation
- Aquatic Invertebrate Ecology and Behavior
- Agroforestry and silvopastoral systems
- Genetic diversity and population structure
- Environmental Toxicology and Ecotoxicology
- Forest Insect Ecology and Management
- Water Treatment and Disinfection
- Marine Bivalve and Aquaculture Studies
- Constructed Wetlands for Wastewater Treatment
- Soil and Water Nutrient Dynamics
- Environmental DNA in Biodiversity Studies
- Genomics and Phylogenetic Studies
Akita Prefectural University
2015-2025
Northern Arizona University
2023
Nutrasource
2011
University of Tsukuba
2003-2009
Abstract The rapid development of DNA sequencing technology in recent years has provided new tools for phylogenetic data acquisition. By using high‐throughput technology, molecular information can be obtained more quickly and economically. Here, we describe a complementary combination two multiplex methods. One is multiplexed marker (MPM‐seq), the other inter‐simple sequence repeat (ISSR) genotyping by (MIG‐seq), whose protocol improved over that original one. Both MPM‐seq MIG‐seq begin with...
mlrA is the only microcystin-degrading gene detected in Sphingomonas sp. MJ-PV. The has an extremely rare nucleotide sequence and homologous genes have not yet been discovered DNA database. We existence of a to new bacteria, MD-1 Y2. These strains possessed homologues, identities Y2 with corresponding MJ-PV exceeded 98% 84%, respectively. On other hand, was laboratory closely related spp. employing hemi-nested polymerase chain reaction detection using two primer sets. Although bacteria were...
Sequence data is now widely used to observe relationships among organisms. However, understanding structure of the qualitative challenging. Conventionally, are analysed using a dendrogram that estimates tree shape. This approach has difficulty in verifying appropriateness shape; rather, horizontal gene transfers and mating can make shape relationship as networks. As connection-free approach, principal component analysis (PCA) summarize distance matrix, which records distances between each...
A novel strictly anaerobic chemoorganotrophic bacterium, designated Mahy22T, was isolated from sulfidic bottom water of a shallow brackish meromictic lake in Japan. Cells the strain were Gram-stain-negative, non-motile and coccoid shape with diameters about 600-800 nm. The temperature range for growth 15-37 °C, optimum at 30-32 °C. pH 6.2-8.9, 7.2-7.4. grew NaCl concentrations 5% or below (optimum, 2-3%). Growth enhanced by addition thiosulfate. major cellular fatty acids C16:0...
The pH of the water associated with toxic blooms cyanobacteria is typically in alkaline range; however, previously only microcystin-degrading bacteria growing neutral conditions have been isolated. Therefore, we sought to isolate and characterize an alkali-tolerant bacterium from a bloom using microcystin-LR. Analysis 16S rRNA gene sequence revealed that isolated belonged genus Sphingopyxis, strain was named C-1. Sphingopyxis sp. C-1 can grow; at 11.0; optimum for growth 7.0. microcystin...
Abstract Understanding how ecosystem multifunctionality is maintained in naturally assembled communities crucial, because human activities benefit from multiple functions and services of various ecosystems. However, the effects above‐ below‐ground biodiversity on alpine boreal moorland ecosystems remain unclear despite their potential as global carbon sinks. Here we evaluated related to primary production sequestration, which are crucial for climate regulation, natural systems. We...
Abstract Odours in waters used for various purposes, especially musty odours, is a worldwide problem. To establish an early warning system, the nuisance odour aquatic environment, causative microorganisms and compounds, mechanism of Lake Kasumigaura, second biggest lake Japan, were examined using situ studies. In this study, occurrence odours was determined, level discriminated, based on 32 months phytoplankton data from three sampling stations. The results indicate that cell numbers...
Abstract Alkali tolerance and the mechanism of microcystin (MC) degradation were investigated in MC‐degrading bacterial species, Sphingopyxis sp. C‐1, to better understand increased MC under alkaline conditions that arise during disappearance water blooms. bacteria harbour mlrA , mlrB mlrC encode enzymes. C‐1 also possesses these genes, as well mlrD gene has been assumed its transporter. This study demonstrated activity was promoted by intermittent addition microcystin‐LR (MCLR) cultures...
Toxic algae in eutrophic lakes produce cyanotoxic microcystins. Prior research on the effect of microcystin-LR kidney utilized intraperitoneal injections, which did not reflect natural exposure. Oral has focused renal function and histopathology without examining molecular mechanisms. The present study aimed to evaluate mechanism kidneys via oral administration WKAH/HkmSlc rats over 7 weeks, alongside stimulation proximal tubular cells. Although there were no differences concentrations...
This report describes the whole-genome sequence of an alkalitolerant microcystin-degrading bacterium, Sphingopyxis sp. strain C-1, isolated from a lake in China.
Microcystin-degrading bacteria first degrade microcystins by microcystinase A (MlrA) to cleave the cyclic structure of at Adda-Arg site microcystin-LR, microcystin-RR, and microcystin-YR, but cleavage other was not clear. In our study, microcystin-degrading bacterium
Recirculating aquaculture systems (RAS) are important for water quality management in facilities, and can help resume consumption. However, information about the community structure of micro-ecosystem existing biofilters, especially participation known nitrifying groups (i.e., AOA, AOB, NOB, comammox Nitrospira), remains to be fully clarified. In this research, we compared structures three RAS operated at different temperatures a marine aquarium, through both amoA-targeted qPCR assay 16S...
We showed that the photocatalytic effect of a coating TiO2 greatly reduces formation biofilm by Phormidium tenue (P. tenue), filamentous cyanobacterium, on glass plates. Sample plates were immersed in P. culture solution (OD730=0.3) under concurrent illumination with white fluorescent (WF) and UV light (0.3 mW cm-2, each) for 11 days. TiO2-coated reduced adhesion over days compared to bare The number adhering increased 106 cells cm-2 6 anti-biofilm was also observed WF light, although it...
Mine drainage waters often contain elevated concentrations of toxic metals and can be a source environmental pollution. Manganese (Mn)-oxidizing bacteria (MnOB), which oxidize soluble Mn(II) to insoluble Mn(III, IV) under neutral pH conditions, are expected promising biological agent in passive mine remediation. However, the diversity, functionality applicability MnOB for treatment remain mostly unknown. Given that many environments poor organic substances, it is necessary elucidate...
Burkholderiales bacterium GJ-E10, isolated from the Tamagawa River in Akita Prefecture, Japan, is an unclassified, iron-oxidizing chemolithoautotrophic bacterium. Its single circular genome, consisting of 3,276,549 bp, was sequenced by using three types next-generation sequencers and sequences were then confirmed PCR-based Sanger sequencing.
Harmful algae that inhabit eutrophic lakes produce cyanotoxic microcystins. Therefore, the relationship between chronic exposure to microcystins via drinking water and organ disorders has been investigated. The present study aimed determine whether representative microcystin-LR is involved in increased monocyte chemoattractant protein-1 (MCP-1) expression rat colonic mucosa enterocyte-like differentiated Caco-2 cells. mRNA of MCP-1 was colons rats administered with microcystin-LR, compared...