Abstract A key feature that differentiates prokaryotic cells from eukaryotes is the absence of an intracellular membrane surrounding chromosomal DNA. Here, we isolate a member ubiquitous, yet-to-be-cultivated phylum ‘ Candidatus Atribacteria’ (also known as OP9) has intracytoplasmic apparently nucleoid. The isolate, RT761, subsurface-derived anaerobic bacterium appears to have three lipid membrane-like layers, shown by cryo-electron tomography. Our observations are consistent with classical...

ABSTRACT Despite their importance as a biofuel production platform, only very limited number of butanol-tolerant bacteria have been identified thus far. Here, we extensively explored butanol- and isobutanol-tolerant from various environmental samples. A total 16 aerobic anaerobic that could tolerate greater than 2.0% (vol/vol) butanol isobutanol were isolated. 16S rRNA gene sequencing analysis revealed the isolates phylogenetically distributed over at least nine genera: Bacillus ,...

Gas hydrates deposited in subseafloor sediments are considered to primarily consist of biogenic methane. However, little evidence for the occurrence living methanogens has been provided. This study investigated viable methanogen diversity, population, physiology and potential activity hydrate-bearing (1-307 m below seafloor) from eastern Nankai Trough. Radiotracer experiments, quantification coenzyme F430 molecular sequencing analysis indicated methanogenic predominance hydrogenotrophic...

ABSTRACT Phylogenetic analysis of bacteria preserved within an ice wedge from the Fox permafrost tunnel was undertaken by cultivation and molecular techniques. The radiocarbon age determined. Our results suggest that in adapted to frozen conditions have survived for 25,000 years.

A moderately thermophilic, methanol-oxidizing bacterium (strain Gela4(T)) was isolated from methane-utilizing mixed-culture originating marine sediment near a hydrothermal vent. Phylogenetic analysis of 16S rRNA gene sequences indicated that strain Gela4(T) closely related to members the genus 'Methyloligella' (94.7% similarity) within class Alphaproteobacteria. Strain Gram-staining-negative and aerobic organism. Cells were rod-shaped non-motile. The temperature range for growth 19-43 °C...

Gram-positive, aerobic, non-spore-forming, irregular rod-shaped bacteria, designated strains AHU1791(T) and AHU1810, were isolated from a permafrost ice wedge in Alaska. Cells motile by means of polar flagellum. The psychrophilic, growing at -5 to 25 degrees C. Phylogenetic analysis 16S rRNA gene sequences indicated that the ice-wedge isolates formed clade distinct other genera affiliated with family Microbacteriaceae. novel showed highest levels sequence similarity members Agreia Subtercola...

A mesophilic, slightly halophilic, obligately methylotrophic, methanogenic archaeon, designated strain GTA13(T), was isolated from natural gas-bearing confined aquifers in the Minami-Kanto gas field, Japan. The cells were non-motile, irregular cocci, 0.7-1.0 µm diameter and occurred singly, pairs or as small aggregates. grew with tri- dimethylamine but not H2/CO2, formate, acetate, methanol dimethyl sulphide. Vitamins, sodium magnesium required for growth. Optimal growth at pH 7.0-7.5, 35...

An obligately anaerobic bacterium, designated strain GK12 T , was isolated from an digester in Fukagawa, Hokkaido Prefecture, Japan. The cells of were non-motile, non-spore-forming cocci that commonly occurred chains. 16S rRNA gene sequence analysis revealed affiliated with the family Erysipelotrichaceae phylum Firmicutes and showed 91.8 % similarity to most closely related species, Faecalicoccus acidiformans . grew at 30–50 °C (optimally 40 °C) pH 5.5–8.5 7.5). main end product glucose...

Little is known about the microbial distribution patterns in subseafloor sediments. This study examines diversity and activities sediments of Nankai Trough, where biogenic gas hydrates are deposited. Illumina sequencing 16S rRNA genes revealed that prokaryotic community structure correlated with hydrate occurrence depth but not sedimentary facies. The bacterial phyla 'Atribacteria' lineage JS1 Chloroflexi dominated all samples, whereas lower taxonomic units accounted for variation related to...

Abstract The Minami-Kanto gas field, where gases are dissolved in formation water, is a potential analogue for marine hydrate area because both areas characterized by the accumulation of microbial methane turbidite sand layers interbedded with mud layers. This study examined physicochemical impacts associated natural production and well drilling on methanogenic activity composition this field. Twenty-four gas-associated water samples were collected from confined aquifers through wells....

Abstract Rainbow trout fry syndrome (RTFS) and bacterial coldwater disease (BCWD) is a globally distributed freshwater fish caused by Flavobacterium psychrophilum . In spite of its importance, an effective vaccine not still available. Manipulation the microbiome skin, which primary infection gate for pathogens, could be novel countermeasure. For example, increasing abundance specific antagonistic bacteria against pathogens in skin might to prevent disease. Here, we combined cultivation with...

Gram-reaction-positive, aerobic, non-spore-forming, irregular rod-shaped bacteria, designated AHU1821(T) and AHU1820, were isolated from an ice wedge in the Fox permafrost tunnel, Alaska. The strains psychrophilic, growing at -5 to 27°C. Phylogenetic analysis of 16S rRNA gyrB gene sequences indicated that ice-wedge isolates formed a clade distinct other mycolic-acid-containing bacteria within suborder Corynebacterineae. cell wall AHU1820 contained meso-diaminopimelic acid, arabinose...

A novel anaerobic, thermophilic bacterium of the class Atribacteria, strain M15T, was isolated from a high-temperature gas reservoir, Japan. Cells M15T were gram-negative, short oval-shaped, and lacked flagella. Growth occurred at 45–75 °C (optimum 70–75 °C) pH 6.5–8.5 7.5–8.0) fast under optimal conditions (doubling time 11.4 h). Yeast extract required for growth. Fermentative growth with glucose, arabinose, xylose, cellobiose observed. The major fermentative end products glucose acetate...

Functional variation of Rpf, a growth factor found exclusively in Actinobacteria, is differentiated by its source and amino acid sequences. Only purified Rpf proteins from three species have been studied so far. To seek new Rpfs for use future studies to understand their role the objective this study was identify rpf gene homologs Tomitella biformata AHU 1821(T), novel Actinobacteria isolated permafrost ice wedge. Amplification using degenerate primers targeting essential domain led...

ABSTRACT Medium- and long-chain fatty acids are present in organisms esterified forms that serve as cell membrane constituents storage compounds. A large number of known to accumulate lipophilic materials a source energy carbon. We found bacterium, designated GK12, intrinsically accumulates free (FFAs) intracellular droplets without exhibiting cytotoxicity. GK12 is an obligatory anaerobic, mesophilic lactic acid bacterium was isolated from methanogenic reactor. Phylogenetic analysis based on...

A moderately thermophilic, aerobic, stalked bacterium (strain MA2T) was isolated from marine sediments in Kagoshima Bay, Japan. Phylogenetic analysis of 16S rRNA gene sequences indicated that strain MA2T most closely related to the genera Rhodobium,Parvibaculum, and Rhodoligotrophos (92-93 % similarity) within class Alphaproteobacteria. Strain a Gram-stain-negative dimorphic bacteria. The temperature range for growth 16-48 °C (optimum at 42 °C). This required yeast extract NaCl (>1 %, w/v)...

A mesophilic, hydrogenotrophic methanogen, designated strain MobHT, was isolated from sediments derived deep sedimentary, natural-gas-bearing aquifers in Japan. Strain MobHT utilized H2/CO2 or formate, but not ethanol, 1-propanol, 2-propanol, 2-butanol cyclopentanol, for growth and methane production. In addition, acetate tungsten were required growth. Yeast extract stimulated the growth, required. The cells weakly motile with multiple flagella, presented as a curved-rod-shaped (0.8×2.0 µm)...

Mine drainage waters often contain elevated concentrations of toxic metals and can be a source environmental pollution. Manganese (Mn)-oxidizing bacteria (MnOB), which oxidize soluble Mn(II) to insoluble Mn(III, IV) under neutral pH conditions, are expected promising biological agent in passive mine remediation. However, the diversity, functionality applicability MnOB for treatment remain mostly unknown. Given that many environments poor organic substances, it is necessary elucidate...

Abstract A diastereomerically mixed complex [Ru{(S)-phgly}2{(±)-biphep}] is readily prepared from achiral diphosphine BIPHEP in two steps. These diastereomers are then separated by silica gel column chromatography. 61:39 equilibrium mixture of [Ru{(S)-phgly}2{(S)-biphep}] and [Ru{(S)-phgly}2{(R)-biphep}] with Li2CO3 used to catalyze cyanosilylation benzaldehyde afford the R cyanated product 92% ee. The enantioselectivity just slightly lower than that using pure...

Abstract A key feature that differentiates prokaryotes from eukaryotes is the absence of an intracellular membrane surrounding chromosomal DNA. Here, we report isolation anaerobic bacterium possesses additional intracytoplasmic a nucleoid, affiliates with yet-to-be-cultivated ubiquitous phylum ‘ Ca . Atribacteria’, and unique genomic features likely associated organization complex cellular structure. Exploration uncharted microorganism overturned prevailing dogma prokaryotic cell