A5063517960- Aquaculture disease management and microbiota
- Vibrio bacteria research studies
- Microbial infections and disease research
- Myxozoan Parasites in Aquatic Species
- Bacteriophages and microbial interactions
- Aquaculture Nutrition and Growth
- Identification and Quantification in Food
- Biosensors and Analytical Detection
- Genomics and Phylogenetic Studies
- Microbial Community Ecology and Physiology
- Physiological and biochemical adaptations
- Microbial Metabolism and Applications
- Photosynthetic Processes and Mechanisms
- Gut microbiota and health
- Aquatic Ecosystems and Phytoplankton Dynamics
- Parasite Biology and Host Interactions
- Meat and Animal Product Quality
- Fecal contamination and water quality
- Algal biology and biofuel production
- Isotope Analysis in Ecology
- Fermentation and Sensory Analysis
- GABA and Rice Research
- Marine Bivalve and Aquaculture Studies
- Probiotics and Fermented Foods
- Infections and bacterial resistance
Kindai University
2010-2024
Virologie et Immunologie Moléculaires
2013
Flavobacterium psychrophilum, the etiological agent of rainbow trout fry syndrome and bacterial cold-water disease in salmonid fish, is currently one main pathogens hampering productivity farming worldwide. In this study, genomic diversity F. psychrophilum species analyzed using a set 41 genomes, including 30 newly sequenced isolates. These were selected on basis available MLST data with two-fold objective maximizing coverage allowing focus clonal complex (CC-ST10) infecting farmed...
The bacterium Flavobacterium psychrophilum is a serious problem for salmonid farming worldwide. This study investigates by multilocus sequence typing (MLST) the population structure of this pathogen in Japan where it also major concern ayu, popular game fish related to salmoniforms. A total 34 isolates collected across country and 80 sampled single model river electrofishing were genotyped. data accounting 15 species allowed identifying 35 distinct types (ST) Japan. These ST are from those...
Flavobacterium psychrophilum is a devastating bacterial pathogen of salmonids reared in freshwater worldwide. So far, serological diversity between isolates has been described but the underlying molecular factors remain unknown. By combining complete genome sequence analysis and serotyping method proposed by Lorenzen Olesen (1997) for set 34 strains, we identified key determinants serotypes. This knowledge allowed us to develop robust multiplex PCR-based scheme, which was applied 244...
Abstract Rainbow trout fry syndrome (RTFS) and bacterial coldwater disease (BCWD) is a globally distributed freshwater fish caused by Flavobacterium psychrophilum . In spite of its importance, an effective vaccine not still available. Manipulation the microbiome skin, which primary infection gate for pathogens, could be novel countermeasure. For example, increasing abundance specific antagonistic bacteria against pathogens in skin might to prevent disease. Here, we combined cultivation with...
Abstract We developed a simple genotyping method for Flavobacterium psychrophilum analysing two single nucleotide polymorphisms (SNPs) in the gyrA gene and to distinguish between isolates that are virulent avirulent ayu, Plecoglossus altivelis (Temminck & Schlegel). The is an on/off switch assay based on polymerase chain reaction technique with phosphorothioated primers. classified 232 from four families of fish (i.e. Plecoglossidae, Osmeridae, Cyprinidae Salmonidae) into genotypes (G‐C,...
Bacterial cold-water disease (BCWD) causes heavy mortality of ayu Plecoglossus altivelis in Japan. To control BCWD ayu, warmed water treatment at 28°C has been used. However, the mechanism this is unknown. Hence, we investigated growth/survival Flavobacterium psychrophilum 15-28°C under two nutritional conditions: modified cytophaga (MCY) broth and sterilized underground water. Within 2 days both conditions, F. totally lost its colony-forming ability. We also studied distributions infected...
Flavobacterium psychrophilum is the causative agent of bacterial cold-water disease and rainbow trout fry syndrome salmonids. The pathogen has been reported from all regions in world involved salmonid aquaculture, but also natural fresh-water environments. We established a quantitative loop-mediated isothermal amplification DNA (LAMP) method to estimate quantities F. psychrophilum. LAMP primers were designed based on sequence topoisomerase IV subunit B gene, parE, parE exhibited high...
Flavobacterium psychrophilum, the causative agent of bacterial cold-water disease, is a devastating, worldwide distributed, fish pathogen causing significant economic loss in inland farms. Previous epidemiological studies showed that prevalent clonal complexes (CC) differ species affected with disease such as rainbow trout, coho salmon and ayu, indicating associations between particular F. psychrophilum genotypes host species. Yet, whether population structure driven by trade eggs or...
ABSTRACT With the rapid growth of inland aquaculture worldwide, side effects such as discharge nutrients and antibiotics pose a threat to global environments. A sustainable future for requires an effective management system, including early detection disease through monitoring specific biomarkers in tanks. To this end, we investigated whether fish feces tanks could be used non-invasive health using ayu ( Plecoglossus altivelis ) infected with Flavobacterium psychrophilum , which causes...
Bacterial cold-water disease (BCWD) caused by Flavobacterium psychrophilum leads to heavy mortality of ayu Plecoglossus altivelis in Japan. Previously, a 28°C warmed water treatment was shown be an effective for BCWD, since F. can not grow and maintain colonies at this temperature. However, it unclear whether the bacteria might resuscitate after over. Therefore, we investigated colony formation, membrane potential pathogenicity various temperatures (15°C, 23°C, 33°C) sterilized underground...
The activity of membrane-bound NADH oxidase Vibrio anguillarum M93 (serotype J-O-1), which causes vibriosis in freshwater area was activated by Na(+) the same manner as other marine Vibrios. However, addition to Na(+), K(+) also found positively enhance strain M93. This tendency has not been recognized Furthermore, Na(+)-dependent required less (0.1 M) for its maximum than those Vibrios such alginolyticus and 'Vibrio angustum' S14, were range 0.4 M NaCl, similar seawater. Destruction H(+)...
Vibrio anguillarum kills various kinds of fish over salinities ranging from seawater to freshwater. In this study, we investigated the role Na(+) in V. anguillarum, especially under energy-depleted conditions such as natural seawater. angustum S14, which is a typical marine vibrio, was used for comparison. only required starvation-survival, but contrast, S14 always both growth and starvation-survival. vibrios, Na(+)-dependent respiratory chain that produces sodium motive force (SMF) across...
Vibrio anguillarum kills various kinds of fish over salinities ranging from seawater to freshwater. In this study, we investigated the role Na+ in V. anguillarum, especially under energy-depleted conditions such as natural seawater. angustum S14, which is a typical marine vibrio, was used for comparison. only required starvation-survival, but contrast, S14 always both growth and starvation-survival. vibrios, Na+-dependent respiratory chain that produces sodium motive force (SMF) across cell...
Abstract Flavobacterium psychrophilum can be divided into three genotypes, G-C type (ayu type), A-C (multi-fish and A-T (salmon-trout by two single-nucleotide polymorphisms in the DNA gyrase subunit A ( gyrA ) gene. We isolated F. from various samples collected lower basin of a river flowing Lake Biwa, as model for bacterial survey, Shiga Prefecture June, September, December 2010 to 2013 investigated their genotypes. All types were June when ayu went up lake. In gathered spawning became high...
Phosphorothioate modification of DNA and the corresponding degradation (Dnd) phenotype that occurs during gel electrophoresis are caused by dnd genes. Although widely distributed among Bacteria Archaea, genes have been found in only very few, taxonomically unrelated, bacterial species so far. Here, we report presence their associated Dnd two Flavobacterium species. Comparison with gene clusters previously described led us to a noncanonical genetic organization identify likely encoding hybrid...
Vibriosis caused by Vibrio anguillarum serotype J-O-1 seriously affects the freshwater fish ayu (Plecoglossus altivelis) in Lake Biwa, Japan. Survival patterns of V. were investigated aged lake water (ALW) supplemented with or without 0.75% NaCl. It was found that 0.1-1.0% cells maintained ability to form colonies even after 6 weeks NaCl-ALW. Under same starved conditions, MPN counts liquid medium 100 times higher than CFU counts. When exposed sterilized NaCl, entered a non-culturable state...
Vibriosis caused by Vibrio anguillarum seriously injures freshwater fish (Salmoniforms) almost every year in Lake Biwa, Japan. This pathogen needs NaCl for its growth and survival. When the was directly exposed to sterilized aged lake water (ALW) at room temperature, it suddenly lost culturability pathogenicity, died within half a day due low osmolarity. In this report, survival of as biofilms formed on air-solid liquid-solid (agar or polystyrene) interfaces ALW investigated. were 4-5°C...
Flavobacterium psychrophilum can infect rainbow trout Oncorhynchus mykiss and ayu Plecoglossus altivelis, detrimentally affecting global aquaculture. This study aimed to understand the infectivity host specificity of two F. strains, SG950607 PH0424, which were isolated from kidneys ayu, respectively. Rainbow (fry) (adult fry) infected via methods: bath immersion intraperitoneal injection. We investigated ability each strain survive in serum non-original fish species. died upon being by...
高知県全域の複数河川において，2007-2019年に，アユ死亡魚と発症魚から冷水病菌をそれぞれ28株および10株分離した。分離した冷水病菌の遺伝子型をPCR-RFLP，MLST解析等を用いて，10遺伝子によって判別した。その結果，分離された冷水病菌の遺伝子型はG-C/A/S/52型が多かった。また，県内河川の冷水病発生数は2015年以降に増加し，2018年に最も多かった。2015年以降の死亡魚由来の菌は殆どがG-C/A/S/52型であり，近年の冷水病発生はこの型が主因と考えられた。
"ふなずし"は,琵琶湖周辺で漬けられているなれずしの1種である.伝統的な食文化を継承するため,現代人に好まれる製造方法の解明が求められている.本研究では,嗜好性の高いふなずしの生産管理に向け,品評会に出品されたふなずしを対象として細菌叢と香気成分を分析し,官能評価スコアと併せて評価することを目的とした.ふなずしの細菌叢は,Lactobacillus spp.,Lb. acidipiscis,Lb. buchneri,Lb. brevis,Bacillus spp.,Staphylococcus spp.を主要とする6グループに大別できた.ふなずしから検出された香気成分は3グループに分けられ,それぞれ品評会スコアとおよそ対応していた.品評会で高評価を得たふなずしは清酒と共通の香気成分が多く,低評価のものは腐敗臭成分が多く検出され,嗜好性は香気成分でおよそ説明可能であることが示された.一方で細菌叢と香気成分の明確な関係性はみられず,酵母を含めた発酵過程における経時的な解析が必要となった.