The epithelial–mesenchymal transition (EMT) is an important pathological process in the occurrence of pulmonary fibrosis. Changes histone methylation modifications key genes play role this process. As a methyltransferase, regulatory mechanism and SET domain bifurcated 1 (SETDB1) fibrosis remain unclear. We found that SETDB1 inhibited EMT cells attenuated expression to relieve inhibition during transforming growth factor-β (TGF-β)-induced EMT. Silencing significantly enhanced mesenchymal...

Air purifiers should pay much attention to hospital-associated infections, but the role of a single air purifier is limited. The goal this study was evaluate effectiveness combined application nonequilibrium positive and negative oxygen ion (PNOI) high-efficiency particulate filter (HEPA) on complex, polluted environment. Two better performing were selected before study. efficacy their use alone in combination for purification cigarette matter (PM), Staphylococcus albicans, influenza virus...

Alzheimer's Disease (AD) is a neurodegenerative disease featured by cognitive impairment. This bioinformatic analysis was used to identify hub genes related dysfunction in AD. The gene expression profile GSE48350 the hippocampus of AD patients aged >70 years obtained from Gene Expression Omnibus (GEO) database. A total 96 differentially expressed (DEGs) were identified, and subjected Ontology (GO), Kyoto Encyclopedia Genes Genomes (KEGG) pathway enrichment analyses; protein-protein...

Background:The hotspot regulatory region mutations of the TERT, PLEKHS1 and GPR126 genes have been shown to occur frequently in urothelial bladder carcinoma (UBC).However, it is currently unclear whether these are all present upper tract carcinomas (UTUC) including renal pelvic (RPC) ureter (UC), although TERT promoter were previously observed malignancies.Methods: The promoters intron 6 (enhancer) tumors derived from 164 patients with UTUC determined using Sanger sequencing, obtained...

Idiopathic pulmonary fibrosis (IPF) is a chronic respiratory disease with high incidence, morbidity, and mortality rates. Jinshui Huanxian formula (JHF) an empirical that targets the pathogenesis of lung-kidney qi deficiency phlegm-blood stasis in (PF). The purpose this study was to explore JHF's potential pharmacological mechanisms IPF therapy using network intersection analysis. primary active components corresponding target genes were predicted various databases. Two sets obtained from...

Phosphogluconate dehydrogenase (PGD) is involved in the regulation of various tumors. However, its role hepatocellular carcinoma (HCC) poorly understood. This study tried to determine prognostic efficacy PGD and value for immunotherapy HCC. The data from TCGA database was used explore predictive power expression methylation on overall survival (OS) HCC through Cox regression Kaplan-Meier analysis. Then, we GEO ICGC further verify power. Finally, relationship between immune cells were...

The incidences of nosocomial infections (NIs) are increasing throughout the world, especially for those airborne diseases caused by pathogens or air particulates that float in air. In this study, we designed and manufactured a desk clinic consultation room purification isolation between doctor patient. (AIP) has high efficiency particulate (HEPA) filter on tope several primary filters sides purification. circulating inlet outlet forms wind-curtain Computational Fluid Dynamics (CFD) model was...

Background: Most of the current research on prognostic model construction for non-small cell lung cancer (NSCLC) only involves in bulk RNA-seq data without integration single-cell (scRNA-seq) data.Besides, most models are constructed by predictive genes, ignoring other variables such as clinical features.Methods: We obtained scRNA-seq from GEO database and TCGA database.We construct a through Least Absolute Shrinkage Selection Operator (LASSO) Cox regression.Furthermore, we performed...

Vascular endothelial cells maintain the vascular integrity and play a vital role in acting as an intravascular extravascular barrier. Pyroptosis, discovered recent years, is new form of programmed cell death accompanied by inflammatory reaction immune response. Sigma 1R，distributed different cells, opioid receptor shows multifunctional features. However, sigma 1R COPD remains obscure. This project investigates pyroptosis occurrence endothelium pathogenesis induced cigarette smoke extract...

For a long time, the dispute about whether improved glycemic control can bring significant benefits has remained unresolved. We aimed to investigate association of time spent in different fasting glucose target ranges with cardiovascular risk and all-cause mortality population type 2 diabetes (T2DM).

Recurring episodes of fever characterize tumor necrosis factor receptor-associated periodic syndrome (TRAPS) which is autosomal dominant. The primary symptoms patients with TRAPS include prolonged fever, abdominal pain, muscle and skin rashes. prevalence appeared higher in Western countries than Asian countries. Herein, we present the case a 13-year-old girl who experienced intermittent for 8 years, that occur every 2 years. patient demonstrated headache, vomiting, rash, elevated...

18F-DCFPyL prostate-specific membrane antigen (PSMA) PET/CT is commonly applied to locate lesions of prostate cancer (PCa), but its diagnostic function quantitative parameters ignored. Our study evaluates the intraprostatic PSMA uptake in patients newly diagnosed with PCa and explores their predictive value risk classification, which similar D'Amico criteria.We quantified maximal standardized (SUVmax), mean SUV (SUVmean), total lesion (TL)-PSMA, prostate/muscle (P/M) ratio primary tumor,...

3-phosphoinositide dependent kinase-1 (PDK1) has been shown to be a critical regulator of the PI3K-Akt pathway. PDK1 can activate Akt and participates in activation signaling pathway promote tumor development, invasion metastasis. At present, it found that is highly expressed head neck cancer, multiple myeloma, pancreatic esophageal colon cancer other malignant tumors. Thereby inhibiting overexpression may provide new breakthrough for treatment tumor. many kinds inhibitors have put into...

Abstract Background: Airborne transmission is an important mechanism of spread for both viruses and bacteria in hospitals, with nosocomial infections putting a great burden on public health. Patients intensive care units are highly susceptible these by necessity already have incur high operating costs. Therefore, there urgent need economical medical device to prevent infections. In order achieve this goal, innovative pediatric isolation bed was developed. Methods: We investigated the...

Abstract Background Idiopathic pulmonary fibrosis (IPF) is a chronic respiratory disease with high incidence rate, morbidity and mortality. Jinshui Huanxian formula (JHF) an empirical for the pathogenesis of lung-kidney qi deficiency phlegm-blood stasis in fibrosis. The purpose this study to explore pharmacological mechanism JHF action IPF therapy by network interaction analysis. Methods main active components corresponding target genes were predicted using various databases. Two sets...

Patients with acute infectious respiratory illness may emit bio-aerosols containing pathogens to infect susceptible hosts, including other patients and healthcare workers. This study aimed design the pediatric isolation (PI) bed evaluate its purification effect on aerosols microorganisms in air experiment rooms verify general wards' efficiency. The PI is connected a device that surrounds child's head prevent contaminants' spread into room. We test of room verified hospital. results showed...