A5000892115- Antibiotic Resistance in Bacteria
- Bacterial biofilms and quorum sensing
- CRISPR and Genetic Engineering
- Vibrio bacteria research studies
- Pharmaceutical and Antibiotic Environmental Impacts
- Antibiotic Use and Resistance
- Antibiotics Pharmacokinetics and Efficacy
- Bacterial Identification and Susceptibility Testing
- Plant Pathogenic Bacteria Studies
- Antimicrobial Resistance in Staphylococcus
- Infections and bacterial resistance
- Burkholderia infections and melioidosis
- Fish biology, ecology, and behavior
- Pneumocystis jirovecii pneumonia detection and treatment
- Inhalation and Respiratory Drug Delivery
- Cassava research and cyanide
- Genomics and Phylogenetic Studies
- Enterobacteriaceae and Cronobacter Research
Fundação Oswaldo Cruz
2014-2024
Faculdades Oswaldo Cruz
2019
Universidade do Estado do Rio de Janeiro
2019
The study investigated the genetic relationship of carbapenem-resistant Acinetobacter baumannii clinical isolated from inpatients during 2008-2011 11 Brazilian states. Antimicrobial susceptibility profile was determined by disc diffusion method and Etest. Polymerase chain reaction applied for carbapenemase genes, ISAba1. Isolates were subjected to pulsed field gel electrophoresis (PFGE) multilocus sequence typing (MLST) molecular typing. Most isolates showed high resistance rates antibiotics...
Abstract Background Carbapenemase production is a global public health threat. Antimicrobial resistance (AMR) data analysis critical to policy. Here we analyzed carbapenemase detection trends using the AMR Brazilian Surveillance Network. Methods from hospitals included in laboratory information system dataset were evaluated. The rate (DR) was defined as detected by gene tested per isolate year. temporal estimated Prais–Winsten regression model. impact of COVID-19 on genes Brazil determined...
New Delhi metallo-β-lactamase (NDM)-producing bacteria have been identified at a worrying rate in Brazil since 2013. Owing to the need understand extent of their spread, this study reports dissemination blaNDM different species Gram-negative bacilli regions and states Brazil. A total 81 isolates from nine were studied, including 11 species. All carried blaNDM-1 variant considered multidrug resistant. Colistin amikacin agents with higher activity compared other drugs tested. The findings...
In Brazil, the production of KPC-type carbapenemases in Enterobacteriales is endemic, leading to widespread use polymyxins. present study, 502 Klebsiella pneumoniae isolates were evaluated for resistance polymyxins, their genetic determinants and clonality, addition presence carbapenem genes evaluation antimicrobial resistance. Resistance colistin (polymyxin E) was through initial selection on EMB agar containing 4% sulfate, followed by Minimal Inhibitory Concentration (MIC) determination...
Abstract Background The Brazilian endemic clone Pseudomonas aeruginosa ST277 carries important antibiotic resistance determinants, highlighting the gene coding for SPM-1 carbapenemase. However, and persistence of this is apparently restricted to territory. To understand differences between strains from those isolated in other countries, we performed a phylogenetic analysis 47 P. genomes as well analyzed virulence profiles. Furthermore, evaluated distribution genomic islands assessed detail...
Multidrug-resistant microorganisms are a well-known global problem, and gram-negative bacilli top-ranking. When these pathogens associated with bloodstream infections (BSI), outcomes become even worse. Here we applied whole-genome sequencing to access information about clonal distribution, resistance mechanism diversity other molecular aspects of (GNB) isolated from in Brazil. It was possible highlight international high-risk clones circulating the Brazilian territory, such as CC258 for...
BACKGROUND Polymyxins are currently used as a "last-line" treatment for multidrug-resistant Gram-negative infections. OBJECTIVES To identify the major mechanisms of resistance to polymyxin and compare genetic similarity between multi-drug resistant Klebsiella pneumoniae strains recovered from inpatients public hospitals in Mid-West Brazil. METHODS 97 carbapenems non-susceptible K. were studied. β-lactamases (bla OXA-48, bla KPC, NDM, CTX-M, SHV, TEM, IMP, VIM) mcr-1 mcr-5 genes investigated...
Emergence of colistin-resistant bacteria harboring mobile colistin resistance genes (mcr genes) pose a threat for food-producing animals and humans. In this article, we aim to highlight the emergence Escherichia fergusonii as an important new reservoir mcr-1-harboring plasmid in poultry production. Three strains closely related were isolated from cloacal swabs. Their genome contains four plasmids, including 182,869 bp IncHI2 gene mcr-1. These results will contribute our understanding...
The high rates of carbapenem resistance among Brazilian Pseudomonas aeruginosa isolates are mainly associated with the clone ST277 producing carbapenemase SPM-1. Here, complete genetic composition a IncP plasmid harboring blaKPC-2 in this endemic carrying chromosomal blaSPM-1 was described using whole genome sequencing. These results confirm association these two carbapenemases and also describe novel blaKPC-2-plasmid. Considering fact that occurs high-risk clone, monitoring dissemination...
Nosocomial bacterial infections caused by carbapenem-resistant Klebsiella pneumoniae (CRKP) is associated with high mortality in neurosurgical patients. There are few reports the literature on meningitis CRKP. We report two cases of CRKP after neurosurgery. The K. identification and antimicrobial susceptibility testing were performed using Vitek Compact System. Minimum inhibitory concentrations polymyxin B determined broth microdilution method. Molecular typing isolates was investigated...
This study was conducted to determine the molecular epidemiology of blaKPC-encoding Klebsiella pneumoniae recovered from three public hospitals in Brazil. Molecular investigation blaOXA-48, blaKPC, blaNDM, blaCTX-M, blaSHV, blaTEM, blaIMP, and blaVIM resistance genes performed 99 K. isolates inpatients intensive care units. Antimicrobial susceptibility determined with a Vitek-2 System, except for polymyxin B, which evaluated by microbroth dilution test. Clonal relatedness established...
Abstract Rationale: Melioidosis is an emerging infectious disease in Brazil and caused by Burkholderia pseudomallei, with high morbidity mortality rates. A total of 28 melioidosis cases were reported until 2015. The majority the Northwest region such not previously detected Midwest Brazil. Patient concerns: 42-year-old man was admitted a non-productive cough, dyspnea, myalgia, diffuse abdominal pain. Pulmonary auscultation revealed vesicular murmur, snoring sounds, presence basal crackling...
Due to recent developments in NGS technologies, genome sequencing is generating large volumes of new data containing a wealth biological information. Understanding sequenced genomes biologically meaningful way and delineating their functional metabolic landscapes first-level challenge. Considering the global antimicrobial resistance (AMR) problem, investments expand surveillance improve existing analysis technologies are pressing. In addition, speed at which genomic generated surpasses our...
Pseudomonas aeruginosa, an opportunistic pathogen often found in Healthcare-associated infections (HAI), has shown increased resistance to carbapenems (imipenem, meropenem, doripenem), the primary treatment options. We've seen a rise carbapenemase-producing P. aeruginosa Brazil, including NDM-producers. This study characterises isolate carrying bla
Abstract Background The Brazilian endemic clone Pseudomonas aeruginosa ST277 carry important antibiotic resistance determinants, highlighting the gene coding for SPM-1 carbapenamase. However, and persistence of this is apparently restricted to territory. To completely understand differences between strains from Brazil ones isolated in other countries, we phylogenetically examined 43 P. genomes as well analyzed virulence genes, evaluated distribution genomic islands assessed detail...