A5029589860- Gut microbiota and health
- Vibrio bacteria research studies
- Probiotics and Fermented Foods
- Bacterial biofilms and quorum sensing
- Bacterial Genetics and Biotechnology
- Salmonella and Campylobacter epidemiology
- Mycobacterium research and diagnosis
- Clostridium difficile and Clostridium perfringens research
- Metabolomics and Mass Spectrometry Studies
- Tuberculosis Research and Epidemiology
- Bacterial Identification and Susceptibility Testing
- Antibiotic Resistance in Bacteria
- Diet and metabolism studies
- Streptococcal Infections and Treatments
- Escherichia coli research studies
- Leprosy Research and Treatment
- Antimicrobial Peptides and Activities
- Oral microbiology and periodontitis research
- Sphingolipid Metabolism and Signaling
- Child Nutrition and Water Access
- Infectious Diseases and Tuberculosis
- Legionella and Acanthamoeba research
- Biochemical and Molecular Research
- Antimicrobial Resistance in Staphylococcus
- Listeria monocytogenes in Food Safety
Fundação Oswaldo Cruz
2014-2023
University of Kansas
2023
Instituto Nacional de Ciência e Tecnologia de Doenças Tropicais
2014-2022
Escola Nacional de Saúde Pública
2013-2021
Ministério da Saúde
2021
University of British Columbia
2009-2017
Canada's Michael Smith Genome Sciences Centre
2010-2017
National Council for Scientific and Technological Development
2013
University of Iowa
2007-2011
University of Washington
2007-2011
Gut microbiota is an assortment of microorganisms inhabiting the length and width mammalian gastrointestinal tract. The composition this microbial community host specific, evolving throughout individual's lifetime susceptible to both exogenous endogenous modifications. Recent renewed interest in structure function “organ” has illuminated its central position health disease. intimately involved numerous aspects normal physiology, from nutritional status behavior stress response. Additionally,...
The importance of the mammalian intestinal microbiota to human health has been intensely studied over past few years. It is now clear that interactions between hosts and their associated microbial communities need be characterized in molecular detail if we are truly understand physiology. Additionally, study such host-microbe likely provide us with new strategies manipulate these complex systems maintain or restore homeostasis order prevent cure pathological states. Here, describe use...
The intestinal microbiota is composed of hundreds species bacteria, fungi and protozoa critical for numerous biological processes, such as nutrient acquisition, vitamin production, colonization resistance against bacterial pathogens. We studied the role on host to Salmonella enterica serovar Typhimurium-induced colitis. Using multiple antibiotic treatments in 129S1/SvImJ mice, we showed that disruption alters susceptibility infection. Although all caused similar increases pathogen...
In Vibrio parahaemolyticus, scrC participates in controlling the decision to be a highly mobile swarmer cell or more adhesive, biofilm-proficient type. mutants display decreased swarming motility over surfaces and enhanced capsular polysaccharide production. ScrC is cytoplasmic membrane protein that contains both GGDEF EAL conserved domains. These domains have been shown many organisms respectively control formation degradation of small signaling nucleotide cyclic dimeric GMP (c-di-GMP). The...
We recently showed that Mycobacterium leprae (ML) is able to induce lipid droplet formation in infected macrophages. herein confirm cholesterol (Cho) one of the host molecules accumulate ML-infected macrophages and investigate effects ML on cellular Cho metabolism responsible for its accumulation. The expression levels LDL receptors (LDL-R, CD36, SRA-1, SR-B1, LRP-1) enzymes involved biosynthesis were investigated by qRT-PCR and/or Western blot shown be higher lepromatous leprosy (LL)...
The Vibrio fischeri quorum-sensing signal N-3-oxohexanoyl-l-homoserine lactone (3OC6-HSL) activates expression of the seven-gene luminescence operon. We used microarrays to unveil 18 additional 3OC6-HSL-controlled genes, 3 which had been identified by other means previously. show most these genes are regulated 3OC6-HSL-responsive transcriptional regulator LuxR directly. This demonstrates that V. quorum sensing regulates a substantial number than those involved in light production.
The human microbiome, especially in the intestinal tract has received increased attention past few years due to its importance numerous biological processes. Recent advances DNA sequencing technology and analysis now allow us better determine global differences composition of gut microbial population, ask questions about role health disease. Thus far, roles these commensal bacteria on nutrient acquisition, vitamin production, development have been identified [1]. Furthermore, resistance or...
The Vibrio parahaemolyticus Scr system modulates decisions pertinent to surface colonization by affecting the cellular level of cyclic dimeric GMP (c-di-GMP). In this work, we explore scope and mechanism regulation. Transcriptome comparison ΔscrABC wild-type strains revealed expression differences with respect ∼100 genes. Elevated c-di-GMP repressed genes in surface-sensing regulon, including those encoding lateral flagellar type III secretion systems N-acetylglucosamine-binding protein GpbA...
The interplay between pathogens and their hosts has been studied for decades using targeted approaches, such as the analysis of mutants host immunological responses. Although much learned from studies, they focus on individual pathways fail to reveal global effects infection host. To alleviate this issue, high-throughput methods, transcriptomics proteomics, have used study host-pathogen interactions. Recently, metabolomics was established a new method changes in biochemical composition...
The intestinal microbiota has been found to play a central role in the colonization of Salmonella enterica serovar Typhimurium gastrointestinal tract. In this study, we present novel process through which benefit from inflammatory induced changes order facilitate disease. We show that infection mice causes recruitment neutrophils gut lumen, resulting significant composition microbiota. This occurs production enzyme elastase by neutrophils. Administration recombinant neutrophil infected...
The microbiota influences host health through several mechanisms, including protecting it from pathogen colonization. Staphylococcus epidermidis is one of the most frequently found species in skin microbiota, and its presence can limit development pathogens such as aureusS. aureus causes diverse types infections ranging abscesses to bloodstream infections. Given increasing prevalence S. drug-resistant strains, imperative search for new strategies treatment prevention. Thus, we investigated...
RoundUp® (RUp) is a comercial formulation containing glyphosate (N-(phosphono-methyl) glycine), and the world’s leading wide-spectrum herbicide used in agriculture. Supporters of broad use glyphosate-based herbicides (GBH) claim they are innocuous to humans, since active compound acts on inhibition enzymes which absent human cells. However, neurotoxic effects GBH have already been shown many animal models. Further, these formulations were disrupt microbiome different species. Here, we...
Vibrio fischeri quorum sensing involves the LuxI and LuxR proteins. The protein generates quorum-sensing signal N-3-oxohexanoyl-l-homoserine lactone (3OC6-HSL), is a signal-responsive transcriptional regulator which activates luminescence (lux) genes 17 other V. genes. For activation of lux genes, binds to 20-base-pair inverted repeat, box, centered 42.5 base pairs upstream start operon. Similar box-like elements have been identified in only few LuxR-activated promoters. To better understand...
The mammalian gut contains a complex assembly of commensal microbes termed microbiota. Although much has been learned about the role these in health, mechanisms underlying functions are ill defined. We have recently shown that thousands small molecules, most which currently unidentified. Therefore, we hypothesized molecules function as chemical cues used by hosts and during their interactions health disease. Thus, search was initiated to identify produced microbiota sensed pathogens. found...
To cause disease, Salmonella enterica serovar Typhimurium requires two type III secretion systems that are encoded by pathogenicity islands 1 and 2 (SPI-1 -2). These serve to deliver specialized proteins (effectors) into the host cell cytosol. While importance of these effectors promote colonization replication within has been established, specific roles individual secreted in disease process not well understood. In this study, we used an vivo gallbladder epithelial infection model study...
In Brazil, the production of KPC-type carbapenemases in Enterobacteriales is endemic, leading to widespread use polymyxins. present study, 502 Klebsiella pneumoniae isolates were evaluated for resistance polymyxins, their genetic determinants and clonality, addition presence carbapenem genes evaluation antimicrobial resistance. Resistance colistin (polymyxin E) was through initial selection on EMB agar containing 4% sulfate, followed by Minimal Inhibitory Concentration (MIC) determination...
Despite considerable efforts over the last decades, our understanding of leprosy pathogenesis remains limited. The complex interplay between pathogens and hosts has profound effects on host metabolism. To explore metabolic perturbations associated with leprosy, we analyzed serum metabolome patients. Samples collected from lepromatous tuberculoid patients before immediately after conclusion multidrug therapy (MDT) were subjected to high-throughput profiling. Our results show marked...
Violacein is a violet pigment produced by Chromobacterium violaceum that possesses several functions such as antibacterial, antiviral, antifungal, and antioxidant activities. The search for potential compounds therapies may interfere with modulate the gut microbial consortia without causing severe damage increased resistance important treatment of inflammatory, allergic, metabolic diseases. aim present work was to evaluate ability violacein change patterns in mammalian favoring certain...
ABSTRACT During the colonization of hosts, bacterial pathogens are presented with many challenges that must be overcome for to occur successfully. This requires sensing surroundings and adaptation conditions encountered. One major impediments pathogen mammalian gastrointestinal tract is antibacterial action bile. Salmonella enterica serovar Typhimurium has specific mechanisms involved in resistance Additionally, can successfully multiply bile, using it as a source nutrients. accomplishment...
Chagas disease is a trypanosomiasis whose causative agent the protozoan parasite Trypanosoma cruzi, which transmitted to humans by hematophagous insects known as triatomines and affects large proportion of South America. The digestive tract insect vectors in T. cruzi develops constitutes dynamic environment that development parasite. Thus, we set out investigate chemical composition triatomine intestinal through metabolomics approach. We performed Direct Infusion Fourier Transform Ion...
Adaptation to environments with constant fluctuations imposes challenges that are only overcome sophisticated strategies allow bacteria perceive environmental conditions and develop an appropriate response. The gastrointestinal environment is a complex ecosystem home trillions of microorganisms. Termed microbiota, this microbial ensemble plays important roles in host health provides colonization resistance against pathogens, although pathogens have evolved circumvent barrier. Among the used...