A5053615989- Microbial Community Ecology and Physiology
- Polar Research and Ecology
- Soil Carbon and Nitrogen Dynamics
- Biofuel production and bioconversion
- Mycorrhizal Fungi and Plant Interactions
- Metabolism and Genetic Disorders
- Chromium effects and bioremediation
- Pesticide and Herbicide Environmental Studies
- Gut microbiota and health
- Drug Transport and Resistance Mechanisms
- Environmental and Cultural Studies in Latin America and Beyond
- Biocrusts and Microbial Ecology
- Microbial bioremediation and biosurfactants
- Peroxisome Proliferator-Activated Receptors
- Research on Leishmaniasis Studies
- Geology and Paleoclimatology Research
- Trypanosoma species research and implications
- Ecology and Vegetation Dynamics Studies
- Plant and Fungal Species Descriptions
- Parasites and Host Interactions
- Lichen and fungal ecology
- Environmental Toxicology and Ecotoxicology
- Microbial Metabolic Engineering and Bioproduction
- Enzyme-mediated dye degradation
- Forest Ecology and Biodiversity Studies
Universidad Mayor
2018-2021
Pontificia Universidad Católica de Chile
1999-2017
Institute of Ecology and Biodiversity
2015-2017
GlaxoSmithKline (Spain)
2015
Abstract Using whole-cell phenotypic assays, the GlaxoSmithKline high-throughput screening (HTS) diversity set of 1.8 million compounds was screened against three kinetoplastids most relevant to human disease, i.e. Leishmania donovani , Trypanosoma cruzi and brucei . Secondary confirmatory orthogonal intracellular anti-parasiticidal assays were conducted potential for non-specific cytotoxicity determined. Hit chemically clustered triaged desirable physicochemical properties. The hypothetical...
Summary Ralstonia eutropha JMP134 (pJP4) is a useful model for the study of bacterial degradation substituted aromatic pollutants. Several key degrading capabilities, encoded by tfd genes, are located in 88 kb, self‐transmissible, IncP‐1 β plasmid pJP4. The complete sequence 87 688 nucleotides pJP4, encoding 83 open reading frames (ORFs), reported. Most coding corresponds to well‐conserved backbone and previously reported genes. In addition, we found hypothetical proteins putatively involved...
Summary During soil development, bacteria and fungi can be differentially affected by changes in biogeochemistry. Since the chemistry of parent material affects pH , nutrient availability, indirectly litter quality, we hypothesize that has an important influence on microbial community patterns during long‐term development. In this paper, tested for effect material, as well as, properties upon three c. 20 000‐year‐old semi‐arid chronosequences developed sedimentary volcanic (i.e. Andesitic...
Background and Aims Mycorrhizal associations are influenced by abiotic biotic factors, including climate, soil conditions the identity of host plants. However, effect environmental on orchid mycorrhizal remains poorly understood. The present study examined how differences in nutrient availability related to diversity composition fungi associated with two terrestrial species from central Chile. Methods For 12 populations Bipinnula fimbriata B. plumosa, OTU (operational taxonomic unit)...
The enzymes chlorocatechol-1,2-dioxygenase, chloromuconate cycloisomerase, dienelactone hydrolase, and maleylacetate reductase allow Ralstonia eutropha JMP134(pJP4) to degrade chlorocatechols formed during growth in 2,4-dichlorophenoxyacetate or 3-chlorobenzoate (3-CB). There are two gene modules located plasmid pJP4, tfdC(I)D(I)E(I)F(I) (module I) tfdD(II)C(II)E(II)F(II) II), putatively encoding these enzymes. To assess the role of both tfd degradation chloroaromatics, each module was...
Abstract Natural ecosystems provide services to agriculture such as pest control, soil nutrients, and key microbial components. These others in turn essential elements that fuel biomass productivity. Responsible agricultural management conservation of natural habitats can enhance these ecosystem services. Vineyards are currently driving land‐use changes many Mediterranean ecosystems. could have important effects on the supporting related properties communities associated with forests...
Aims: To study biological removal of the herbicide simazine in soils with different history treatment and to test bioaugmentation a simazine-degrading bacterial strain. Methods Results: Simazine was studied microcosms prepared that had been differentially exposed this herbicide. much higher previously than unexposed ones. Terminal restriction fragment length polymorphism analysis multivariate showed contained communities were significantly impacted by but also an increased resilience. The...
The canalicular multispecific organic anion transporter, cMoat, is an ATP-binding-cassette protein expressed in the domain of hepatocytes. In addition to transport endo- and xenobiotics, cMoat has also been proposed GSH into bile, major driving force bile-acid-independent bile flow. We have shown previously that herbicide 2,4,5-trichlorophenoxyacetic acid (2,4,5-T), a peroxisome-proliferator agent, significantly increases flow mice. On this basis, effect on gene expression was studied. A...
Visceral leishmaniasis (VL) and Chagas disease (CD) are caused by kinetoplastid parasites that affect millions of people worldwide impart a heavy burden against human health. Due to the partial efficacy toxicity-related limitations existing treatments, there is an urgent need develop novel therapies with superior safety profiles successfully treat these diseases. Herein we report application whole-cell phenotypic assays screen set 150,000 compounds Leishmania donovani, causative agent VL,...
In recently deglaciated soils, microbial organisms drive soil transformations by increasing carbon (C) and nitrogen (N) pools while depleting available phosphorous (P), thus improving plant colonization development. However, the rate of development can vary in response to local environmental conditions that affect abundance activity. this contribution we use observational experimental approaches evaluate interplay between biogeochemical features function after approximately seventy years...
Ralstonia eutropha JMP134 (pJP4) grows on 3-chlorobenzoate (3-CB) or 2,4-dichlorophenoxyacetate (2,4-D). The copy number of chlorocatechol genes has been observed to be important for allowing growth bacterial strains chloroaromatic compounds. Despite the fact that two functional degradation tfd gene clusters are harbored plasmid pJP4, a single region comprising all in strain JMP134-F was insufficient allow 3-CB, whereas 2,4-D only slightly retarded compared wild-type strain. Using...
The canalicular multispecific organic anion transporter, cMoat, is an ATP-binding-cassette protein expressed in the domain of hepatocytes. In addition to transport endo- and xenobiotics, cMoat has also been proposed GSH into bile, major driving force bile-acid-independent bile flow. We have shown previously that herbicide 2,4,5-trichlorophenoxyacetic acid (2,4,5-T), a peroxisome-proliferator agent, significantly increases flow mice. On this basis, effect on gene expression was studied. A...