A5019607052- Cancer Genomics and Diagnostics
- Molecular Biology Techniques and Applications
- Prostate Cancer Treatment and Research
- Genomic variations and chromosomal abnormalities
- Gene expression and cancer classification
- BRCA gene mutations in cancer
- HIV Research and Treatment
- Genetic factors in colorectal cancer
- Hepatitis B Virus Studies
- Glycosylation and Glycoproteins Research
- HIV/AIDS Research and Interventions
- Bioinformatics and Genomic Networks
- Tannin, Tannase and Anticancer Activities
- Vaccine Coverage and Hesitancy
- Hepatitis C virus research
- HIV/AIDS drug development and treatment
- Cancer, Lipids, and Metabolism
- PARP inhibition in cancer therapy
- Receptor Mechanisms and Signaling
- Cholesterol and Lipid Metabolism
- PI3K/AKT/mTOR signaling in cancer
- Hepatitis Viruses Studies and Epidemiology
- vaccines and immunoinformatics approaches
- Lipid metabolism and biosynthesis
- Influenza Virus Research Studies
Fred Hutch Cancer Center
2004-2018
Cape Town HVTN Immunology Laboratory / Hutchinson Centre Research Institute of South Africa
2009-2012
Cancer Research Center
2009
Florida College
2003
University of Florida
2003
Methodist Hospital
1991
Baylor College of Medicine
1991
ABSTRACT Characterization of the immune responses induced in initial stages human immunodeficiency virus type 1 (HIV-1) infection is critical importance for an understanding early viral pathogenesis and prophylactic vaccine design. Here, we used sequential plasma samples collected during eclipse exponential expansion phases from subjects acquiring HIV-1 (or, comparison, hepatitis B [HBV]or C [HCV]) to determine nature kinetics earliest systemic elevations cytokine chemokine levels each...
Abstract In this study, we performed high-resolution array comparative genomic hybridization with an of 4153 bacterial artificial chromosome clones to assess copy number changes in 44 archival breast cancers. The tumors were flow sorted exclude non-tumor DNA and increase our ability detect gene changes. these tumors, losses more frequent than gains, gains 1q loss 16q the most alterations. We compared based on histologic subtype estrogen receptor (ER) status, i.e., ER-negative infiltrating...
It is important to identify vaccine-induced immune responses that predict the preventative efficacy of a human immunodeficiency virus (HIV)-1 vaccine. We assessed T-cell response markers as correlates risk in HIV Vaccine Trials Network (HVTN) 505 HIV-1 vaccine trial.2504 participants were randomized DNA/rAd5 or placebo, administered at weeks 0, 4, 8, and 24. Peripheral blood mononuclear cells obtained week 26 from all 25 primary endpoint cases 125 matched controls, stimulated with...
Abstract Androgen deprivation is the mainstay of therapy for progressive prostate cancer. Despite initial and dramatic tumor inhibition, most men eventually fail die metastatic castration-resistant (CR) disease. Here, we characterize profound degree genomic alteration found in CR tumors using array comparative hybridization (array CGH), gene expression arrays, fluorescence situ (FISH). Bycluster analysis, show that similarity profiles from primary driven by patient. Using data adjusted this...
Abstract Disseminated epithelial cells can be isolated from the bone marrow of a far greater fraction prostate-cancer patients than who progress to metastatic disease. To provide better understanding these cells, we have characterized their genomic alterations. We first present an array comparative hybridization method capable detecting changes in small number disseminated (10–20) that typically obtained aspirates patients. show multiple regions copy-number change, including alterations...
Five preventative HIV vaccine efficacy trials have been conducted over the last 12 years, all of which evaluated (VE) to prevent infection for a single regimen versus placebo. Now that one these has supported partial VE prime-boost regimen, there is interest in conducting simultaneously evaluate multiple regimens against shared placebo group same geographic region, accelerating pace development. This article proposes such design, main objectives (1) each exposures occurring near time...
Abstract The molecular specificities of plasma lecithin:cholesterol acyltransferase (LCAT) from ten animal species have been compared. Using a reassembled high density lipoprotein containing mixture phosphatidylcholines, the relative rates liberation different cholesteryl ester were measured. All but two LCAT clustered according to one three patterns substrate specificity. six species, including human, did not transfer highly polyunsaturated fatty acyl chains. In addition, human...
Abstract The two main histological types of infiltrating breast cancer, lobular (ILC) and the more common ductal (IDC) carcinoma are morphologically clinically distinct. To assess molecular alterations associated with these cancer subtypes, we conducted a whole‐genome study 166 archival estrogen receptor (ER)‐positive tumors (89 IDC 77 ILC) using Affymetrix GeneChip® Mapping 10K Array to identify sites loss heterozygosity (LOH) that either distinguished, or were shared by, phenotypes. We...
The problem of covariate measurement error with heteroscedastic variance is considered. Standard regression calibration assumes that the has a homoscedastic variance. An estimator proposed to correct coefficients for Point and interval estimates are derived. Validation data containing gold standard must be available. This closed-form correction uncorrected primary coefficients, which may logistic or Cox proportional hazards model form, closely related version developed by Rosner et al....
Abstract BACKGROUND Oralfacial clefting (OFC) disorders require expedient evaluation and treatment to obtain optimal outcome. In Florida, there is a statewide program targeted the care of infants with OFC. We therefore sought determine referral patterns children born OFC identified through Florida Birth Defects Registry. METHODS Using data for 1996 1997 ICD‐9 CM codes 749.00 ‐ 749.25, we 539 cases. All cases were matched records Children's Medical Services' (CMS) craniofacial centers (CFC)...
Accurate safety monitoring in HIV vaccine trials is vital to eventual licensure and consequent uptake of products. Current practice preventive trials, under the Vaccine Trials Network (HVTN), capture related side effects a hardcopy tool. The reconciliation this tool, 2 weeks after vaccination at visit, time consuming, laborious, fraught with error. Unstructured Supplementary Service Data (USSD), commonly used purchase airtime, has been suggested for collection data trials. With saturated...
Multiple antibody specificities (gp41, V1V2, and V3) elicited in the phase II multiclade (A, B, C) HIV-1 DNA prime, rAd5 boost vaccine trial WB Williams, K Jones, A Krambrink, D Grove, P Liu, NL Yates, MA Moody, G Ferrari, J Pollara, Z Moodie, CA Morgan, H Liao, DC Montefiori, C Ochsenbauer, Kappes, S Hammer, Mascola, R Koup, L Corey, Nabel, Gilbert, Churchyard, M Keefer, BS Graham, BF Haynes, GD Tomaras
You have accessJournal of UrologyDiscussed Poster, Sunday, May 22, 2005 8:00 am - 12:00 pm1 Apr 2005220: Array Comparative Genomic Hybridization Analysis Disseminated Epithelial Cells from the Blood and Bone Marrow Men with Clinically Localized Prostate Cancer Ilona N. Holcomb, Martin Kinnunen, Cassandra L. Neal, Douglas I. Grove, Edward W. Arfman, Paul H. Lange, Li Hsu, Jeffrey J. Delrow, Daniel Lin, Barbara Trask, Robert Vessella HolcombIlona Holcomb More articles by this author ,...
<div>Abstract<p>In this study, we performed high-resolution array comparative genomic hybridization with an of 4153 bacterial artificial chromosome clones to assess copy number changes in 44 archival breast cancers. The tumors were flow sorted exclude non-tumor DNA and increase our ability detect gene changes. In these tumors, losses more frequent than gains, gains 1q loss 16q the most alterations. We compared based on histologic subtype estrogen receptor (ER) status,...
<div>Abstract<p>In this study, we performed high-resolution array comparative genomic hybridization with an of 4153 bacterial artificial chromosome clones to assess copy number changes in 44 archival breast cancers. The tumors were flow sorted exclude non-tumor DNA and increase our ability detect gene changes. In these tumors, losses more frequent than gains, gains 1q loss 16q the most alterations. We compared based on histologic subtype estrogen receptor (ER) status,...
Supplementary Figures 1-3 from Comparative Analyses of Chromosome Alterations in Soft-Tissue Metastases within and across Patients with Castration-Resistant Prostate Cancer
Supplementary Figure 1 from Array Comparative Genomic Hybridization Analysis of Alterations in Breast Cancer Subtypes
Supplementary Figure 1 from Array Comparative Genomic Hybridization Analysis of Alterations in Breast Cancer Subtypes
<div>Abstract<p>Androgen deprivation is the mainstay of therapy for progressive prostate cancer. Despite initial and dramatic tumor inhibition, most men eventually fail die metastatic castration-resistant (CR) disease. Here, we characterize profound degree genomic alteration found in CR tumors using array comparative hybridization (array CGH), gene expression arrays, fluorescence <i>in situ</i> (FISH). Bycluster analysis, show that similarity profiles from primary...