A5111679666- Plant Virus Research Studies
- Plant and Fungal Interactions Research
- Animal Virus Infections Studies
- Viral gastroenteritis research and epidemiology
- SARS-CoV-2 and COVID-19 Research
- Plant Disease Management Techniques
- Plant Disease Resistance and Genetics
- Bacteriophages and microbial interactions
- Viral Infections and Immunology Research
- RNA regulation and disease
- Insect-Plant Interactions and Control
- interferon and immune responses
- Plant-Microbe Interactions and Immunity
- Forest Insect Ecology and Management
- Plant Pathogenic Bacteria Studies
- Herpesvirus Infections and Treatments
- Plant Pathogens and Fungal Diseases
- Virus-based gene therapy research
- Cancer-related molecular mechanisms research
- Spectroscopy and Chemometric Analyses
- Toxin Mechanisms and Immunotoxins
- RNA and protein synthesis mechanisms
- Animal Disease Management and Epidemiology
- Transgenic Plants and Applications
- Ferroptosis and cancer prognosis
Yangtze University
2016-2025
Nanyang Technological University
2012-2017
Institute of Molecular and Cell Biology
2005-2010
University of Minnesota
2006
China Agricultural University
2003
Cytoplasmic stress granules (SGs) are generally triggered by stress-induced translation arrest for storing mRNAs. Recently, it has been shown that SGs exert anti-viral functions due to their involvement in protein synthesis shut off and recruitment of innate immune signaling intermediates. The largest RNA viruses, coronaviruses, impose great threat public safety animal health; however, the significance coronavirus infection is largely unknown. Infectious Bronchitis Virus (IBV) first...
ABSTRACT The involvement of host proteins in the replication and transcription viral RNA is a poorly understood area for many viruses. For coronaviruses, it was long speculated that giant genome multiple subgenomic mRNA species by unique discontinuous mechanism may require cofactors. To search such cellular proteins, yeast two-hybrid screening carried out using nonstructural protein 14 (nsp14) from coronavirus infectious bronchitis virus (IBV) as bait protein, leading to identification DDX1,...
The N-terminal domain of the coronavirus nucleocapsid (N) protein adopts a fold resembling right hand with flexible, positively charged β-hairpin and hydrophobic palm. This was shown to interact genomic RNA for infectious bronchitis virus (IBV) severe acute respiratory syndrome (SARS-CoV). Based on its 3D structure, we used site-directed mutagenesis identify residues essential RNA-binding activity IBV N viral infectivity. Alanine substitution either Arg-76 or Tyr-94 in led significant...
Avian infectious bronchitis virus (IBV) is a globally prevalent, highly contagious avian pathogen that imposes substantial disease burden and economic losses on the poultry industry. Due to challenges associated with vaccine immunization, there an urgent need develop novel anti-IBV therapeutic strategies. In this study, synthesized compound N-phenethylphenazine-1-carboxamide (SQXA-12) was investigated for its significant inhibitory effects IBV replication. SQXA-12 exhibited low cytotoxicity...
Coronavirus M protein is an essential component of virion and plays pivotal roles in assembly, budding maturation. The integrated into the viral envelope with three transmembrane domains flanked by a short amino-terminal ectodomain large carboxy-terminal endodomain. In this study, we showed co-purification from coronavirus infectious bronchitis virus (IBV) actin. To understand cellular factors that may be involved maturation processes, IBV was used as bait yeast two-hybrid screen, resulting...
Virus-induced apoptosis and viral mechanisms that regulate this cell death program are key issues in understanding virus-host interactions pathogenesis. Like many other human animal viruses, coronavirus infection of mammalian cells induces apoptosis. In study, the global gene expression profiles first determined IBV-infected Vero at 24 hours post-infection by Affymetrix array, using avian infectious bronchitis virus (IBV) as a model system. It reveals an up-regulation transcriptional level...
Coronavirus (CoV) 3C-like proteinase (3CLpro), located in nonstructural protein 5 (nsp5), processes the replicase polyproteins 1a and 1ab (pp1a pp1ab) at 11 specific sites to produce 12 mature proteins (nsp5 nsp16). Structural biochemical studies suggest that a conserved Gln residue P1 position is absolutely required for efficient cleavage. Here, we investigate effects of amino acid substitution 3CLpro cleavage infectious bronchitis virus (IBV) on efficiency viral replication by vitro assays...
The most striking difference between the subgenomic mRNA8 of severe acute respiratory syndrome coronavirus isolated from human and some animal species is deletion 29 nucleotides, resulting in splitting a single ORF (ORF8) into two ORFs (ORF8a ORF8b). ORF8a ORF8b are predicted to encode small proteins, 8a 8b, ORF8 protein, 8ab (a fusion form 8b). To understand functions these we cloned cDNA fragments covering expression plasmids, expressed constructs both vitro vivo systems. Expression...