A5036463011- Animal Virus Infections Studies
- Virology and Viral Diseases
- interferon and immune responses
- Virus-based gene therapy research
- Viral Infections and Vectors
- Viral gastroenteritis research and epidemiology
- Microbial infections and disease research
- HIV Research and Treatment
- Mosquito-borne diseases and control
- Animal Disease Management and Epidemiology
- Bacteriophages and microbial interactions
- RNA regulation and disease
- RNA modifications and cancer
- Immune Response and Inflammation
- Aquaculture disease management and microbiota
- Respiratory viral infections research
- Inflammasome and immune disorders
- Phagocytosis and Immune Regulation
- Ferroptosis and cancer prognosis
- Plant Virus Research Studies
- RNA Research and Splicing
- Animal Nutrition and Physiology
- HIV/AIDS drug development and treatment
- Lipid Membrane Structure and Behavior
- Cellular transport and secretion
Shanghai Veterinary Research Institute
2016-2025
Chinese Academy of Agricultural Sciences
2016-2025
Nanjing Drum Tower Hospital
2013-2023
Michigan State University
2018-2020
University of Georgia
2020
Pir Mehr Ali Shah Arid Agriculture University
2020
Yangzhou University
2009-2018
Cytoplasmic stress granules (SGs) are generally triggered by stress-induced translation arrest for storing mRNAs. Recently, it has been shown that SGs exert anti-viral functions due to their involvement in protein synthesis shut off and recruitment of innate immune signaling intermediates. The largest RNA viruses, coronaviruses, impose great threat public safety animal health; however, the significance coronavirus infection is largely unknown. Infectious Bronchitis Virus (IBV) first...
Abstract Newcastle disease virus (NDV) causes severe infectious in poultry and selectively kills tumor cells, by inducing apoptosis cytokines secretion. In this report, we study the mechanisms underlying NDV-induced investigating unfolded protein response (UPR). We found that NDV infection activated all three branches of UPR signaling (PERK-eIF2α, ATF6, IRE1α) triggered apoptosis, avian cells (DF-1 CEF) various human cancer cell types (HeLa, Cal27, HN13, A549, H1299, Huh7, HepG2)....
ABSTRACT Newcastle disease virus (NDV) is an important avian pathogen. We previously reported that NDV triggers autophagy in U251 glioma cells, resulting enhanced replication. In this study, we investigated whether chicken cells and tissues to enhance demonstrated infection induced steady-state chicken-derived DF-1 primary embryo fibroblast (CEF) evident through increased double- or single-membrane vesicles, the accumulation of green fluorescent protein (GFP)-LC3 dots, conversion LC3-I...
Current studies have revealed the immune effects of graphene oxide (GO) and utilized them as vaccine carriers adjuvants. However, GO easily induces strong oxidative stress inflammatory reaction at site injection. It is very necessary to develop an alternative adjuvant based on derivatives for improving responses decreasing side effects. Carnosine (Car) outstanding safe antioxidant. Herein, feasibility efficiency ultrasmall decorated with carnosine were explored. OVA@GO-Car was prepared by...
Lacking a self-contained metabolism network, viruses have evolved multiple mechanisms for rewiring the metabolic system of their host to hijack host's resources replication. Newcastle disease virus (NDV) is paramyxovirus, as an oncolytic currently being developed cancer treatment. However, how NDV alters cellular still far from fully understood. In this study, we show that infection reprograms cell by increasing glucose utilization in glycolytic pathway. Mechanistically, induces...
Paramyxovirus establishes an intimate and complex interaction with the host cell to counteract antiviral responses elicited by cell. Of various pattern recognition receptors in host, cytosolic RNA helicases interact viral activate mitochondrial signaling protein (MAVS) subsequent cellular interferon (IFN) response. On other hand, viruses explore multiple strategies resist immunity. In this study, we found that Newcastle disease virus (NDV) infection induced MAVS degradation. Further analysis...
The primate lentiviral accessory protein Nef downregulates CD4 and major histocompatibility complex class I (MHC-I) from the cell surface via independent endosomal trafficking pathways to promote viral pathogenesis. In addition, antagonizes a novel restriction factor, SERINC5 (Ser5), increase infectivity. To explore molecular mechanism of Ser5 antagonism by Nef, we determined how affects expression intracellular in comparison MHC-I. We confirm that excludes human immunodeficiency virus type...
A number of new cell death processes have been discovered in recent years, including ferroptosis, which is characterized by the accumulation lipid peroxidation products derived from iron metabolism. The evidence suggests that ferroptosis has a tumor-suppressor function. However, mechanism mediates response tumor cells to oncolytic viruses remains poorly understood. Newcastle disease virus (NDV) can selectively replicate cells. We show NDV-induced acts through p53-SLC7A11-GPX4 pathway....
Newcastle disease virus (NDV), a member of the Paramyxoviridae family, can activate PKR/eIF2α signaling cascade to shutoff host and facilitate viral mRNA translation during infection, however, mechanism remains unclear. In this study, we revealed that NDV infection up-regulated cap-dependent machinery by activating PI3K/Akt/mTOR p38 MAPK/Mnk1 pathways. addition, induced participated 4E-BP1 hyperphosphorylation for efficient protein synthesis when mTOR is inhibited. Furthermore, NP was found...
Nidovirales is one order of RNA virus, with the largest single-stranded positive sense genome enwrapped membrane envelope. It comprises four families ( Arterividae, Mesoniviridae, Roniviridae, and Coronaviridae ) has been circulating in humans animals for almost century, posing great threat to livestock poultry，as well as public health. shares similar life cycle: attachment cell surface, entry, primary translation replicases, viral replication cytoplasm, proteins, virion assembly, budding,...
Newcastle Disease virus (NDV) causes severe and economically significant disease in almost all birds. However, factors that affect NDV replication host cells are poorly understood. generates long double-stranded RNA (dsRNA) molecules during transcription of single-stranded genomic RNA. Protein kinase R (PKR) is activated by dsRNA. The aim this study was to elucidate the role PKR infection. infection led activation dsRNA-dependent phosphorylation its substrate, translation initiation factor...
Newcastle disease virus (NDV) V protein is considered as an effector for IFN antagonism, however, the mechanism remains unknown. In this study, expression of STAT1 and phospho-STAT1 in cells infected with NDV or transfected protein-expressing plasmids were analyzed. Our results showed that targets reduction depends on stimulation IFN-α. addition, a V-deficient genotype VII recombinant strain rZJ1-VS was constructed using reverse genetic technique to confirm results. The lost ability reduce...
Mycoplasma synoviae is an avian pathogen that can lead to respiratory tract infections and arthritis in chickens turkeys, resulting serious economic losses the poultry industry. Enolase reportedly plays important roles several bacterial pathogens, but its role M. has not been established. Therefore, this study, enolase encoding gene (eno) of was amplified from strain WVU1853 expressed E. coli BL21 cells. Then enzymatic activity, immunogenicity binding activity with chicken plasminogen (Plg)...
Restriction factors provide the first line of defense against retrovirus infection by posing several blocks to viral replication cycle. SERINC5 is a novel restriction factor that strongly HIV-1 entry, although it counteracted Nef. Currently, still unclear how entry blocked SERINC5. Notably, this block dependent on Env proteins. Laboratory-adapted strains are sensitive, whereas primary isolates highly resistant proteins mediate virus via extensive conformational rearrangements from closed...
ABSTRACT Ferroptosis, a form of programmed cell death characterized by iron-dependent lipid peroxidation, has recently gained considerable attention in the field cancer therapy. There is significant crosstalk between ferroptosis and several classical signaling pathways, such as Hippo pathway, which suppresses abnormal growth frequently aberrant tumor tissues. Yes-associated protein 1 (YAP), core effector molecule abnormally expressed activated variety malignant We previously proved that...
Pigeon Paramyxovirus Type 1 (PPMV-1), an antigenic and host variant of the Newcastle Disease Virus (NDV), can infect pigeons all ages cause severe economic losses in poultry industry. The existing commercial vaccines are not capable providing complete protection against prevalent PPMV-1 strains. To address this issue, reverse genetic technology was employed to create a recombinant 167DM strain by incorporating chimeric genotype Ⅵ hemagglutinin-neuraminidase (HN) with La Sota as backbone....
The endoribonuclease (EndoU) nsp15 of coronaviruses plays a crucial role in evading host innate immune responses by reducing the abundance viral double-stranded RNA (dsRNA). However, our understanding its interactions with cellular targets remains limited. In this study, we demonstrate that overexpression from four coronavirus genera inhibits protein synthesis and causes nuclear retention PABPC1. Mutation analysis confirms essential EndoU activity these processes. Fluorescence situ...
ABSTRACT It has been reported that lentogenic Newcastle disease virus (NDV) isolates have the potential to become velogenic after their transmission and circulation in chickens, but underlying mechanism is unclear. In this study, a highly NDV variant, JS10-A10, was generated from duck-origin isolate JS10 through 10 consecutive passages chicken air sacs. The properties of selected variant were determined using mean death time (MDT) assays, intracerebral pathogenicity index (ICPI), intravenous...
// Ying Liao 1, * , Hua-Xia Wang 3, Xiang Mao 1 Hongjie Fang 3 Huang Yanrong Li Yingjie Sun Chun Meng Lei Tan Cuiping Song Xusheng Qiu and Chan Ding 2 Department of Avian Diseases, Shanghai Veterinary Research Institute, Chinese Academy Agricultural Sciences, 200241, P. R. China Jiangsu Co-innovation Center for Prevention Control Important Animal Infectious Diseases Zoonoses, Yangzhou 225009, College Medicine, Nanjing University, 210095, These authors have contributed equally as first author...