A5087150211- Influenza Virus Research Studies
- Virology and Viral Diseases
- Animal Virus Infections Studies
- Animal Disease Management and Epidemiology
- Viral gastroenteritis research and epidemiology
- Respiratory viral infections research
- Virus-based gene therapy research
- interferon and immune responses
- Immune Response and Inflammation
- Viral Infections and Vectors
- Viral Infections and Immunology Research
- Vector-Borne Animal Diseases
- vaccines and immunoinformatics approaches
- Cancer-related molecular mechanisms research
- SARS-CoV-2 and COVID-19 Research
- Herpesvirus Infections and Treatments
- RNA modifications and cancer
- Bacteriophages and microbial interactions
- Monoclonal and Polyclonal Antibodies Research
- RNA and protein synthesis mechanisms
- MicroRNA in disease regulation
- HIV Research and Treatment
- COVID-19 Clinical Research Studies
- Microbial infections and disease research
- Mosquito-borne diseases and control
Yangzhou University
2016-2025
Ministry of Agriculture and Rural Affairs
2024-2025
Jiangsu Provincial Center for Disease Control and Prevention
2015-2023
Xiang Yang No.1 People's Hospital
2018-2021
Hubei University of Medicine
2018-2020
Wuyi University
2011
ABSTRACT PA-X is a newly discovered protein that decreases the virulence of 1918 H1N1 virus in mouse model. However, role pathogenesis highly pathogenic avian influenza viruses (HPAIV) H5N1 subtype species totally unknown. By generating two PA-X-deficient and evaluating their different animal models, we show here diminishes HPAIV strain A/Chicken/Jiangsu/k0402/2010 (CK10) mice, chickens, ducks. Expression dampens polymerase activity replication both vitro vivo . Using microarray analysis,...
Lysosomes are acidic organelles that mediate the degradation and recycling of cellular waste materials. Damage to lysosomes can cause lysosomal membrane permeabilization (LMP) trigger different types cell death, including apoptosis. Newcastle disease virus (NDV) naturally infect most birds. Additionally, it serves as a promising oncolytic known for its effective infection tumor cells induction intensive apoptotic responses. However, involvement in NDV-induced apoptosis remains poorly...
To generate a genotype VII Newcastle disease virus (NDV) vaccine with high yield in embryonated chicken eggs, we selected NDV strain JS5/05, which possesses titer embryos as the parental virus. Using reverse genetics, generated genetically tagged derivative (NDV/AI4) of JS5/05 by changing amino acid sequence cleavage site F0 protein. Pathogenicity tests showed that NDV/AI4 was completely avirulent. stable and replicated efficiently during 10 consecutive passages embryos. More importantly,...
Highly pathogenic H5N1 influenza A virus remains a substantial threat to public health. To understand the molecular basis and host mechanism for high virulence of viruses in mammals, we compared two isolates which have similar genetic backgrounds but greatly differ their mice. A/Chicken/Jiangsu/k0402/2010 (CK10) is highly pathogenic, whereas A/Goose/Jiangsu/k0403/2010 (GS10) nonpathogenic. We first showed that CK10 elicited more potent innate immune response than did GS10 mouse lungs by...
Newcastle disease virus (NDV) V protein is considered as an effector for IFN antagonism, however, the mechanism remains unknown. In this study, expression of STAT1 and phospho-STAT1 in cells infected with NDV or transfected protein-expressing plasmids were analyzed. Our results showed that targets reduction depends on stimulation IFN-α. addition, a V-deficient genotype VII recombinant strain rZJ1-VS was constructed using reverse genetic technique to confirm results. The lost ability reduce...
The novel H10N3 avian influenza virus (AIV) has infected four individuals since 2021 and caused severe respiratory damage, posing a significant threat to public health. However, its pathogenic mechanisms remain poorly understood. Our findings revealed that infection induces lung damage causes death in mice, even at low doses. elevated levels of multiple pro-inflammatory factors the bronchoalveolar lavage fluid were significantly increased during infection, displaying hallmarks cytokine...
We isolated and identified 201 Newcastle disease viruses (NDVs) from domestic ducks in a 5-year surveillance study at live bird markets Eastern China. Seventy-three of these isolates were characterized biologically genetically. Fusion protein (F) genes amplified by reverse transcription-polymerase chain reaction sequenced. Intracerebral pathogenicity index tests 1-day-old specific-pathogen-free chickens the mean death time embryonated fowl eggs addition to cleavage site analysis F-protein...
Newcastle disease (ND) is one of the most contagious and devastating diseases to poultry in world. The causative agents are virulent strains virus (NDV), which belong genus Avulavirus, sub-family Paramyxoviridae, family Paramyxovirinae. Knowing genomic antigenic characteristics NDVs might contribute ND control China.The results showed that all belonged sub-genotype VIId shared same cleavage site (112)RRQKR/F(117) fusion protein. At least 69 % (38 55) NDV possessed E347K variation...
Abstract The purpose of this study was to investigate the early risk factors for exacerbation coronavirus disease 2019 (COVID‐19) pneumonia. Restrospective analysis clinical data 85 patients infected with severe acute respiratory syndrome 2 (SARS‐CoV‐2), including gender, age, comorbidities, symptoms, blood routine, clotting profile, biochemical examination, albumin, myocardial enzyme inflammatory markers, and chest computed tomography (CT). All laboratory examinations were measured within...
Abstract Since 2014, clade 2.3.4.4 has become the dominant epidemic branch of Asian lineage H5 subtype highly pathogenic avian influenza virus (HPAIV) in southern and eastern China, while H5N6 is most prevalent. We have shown earlier that lack glycosylation at position 158 hemagglutinin (HA) glycoprotein due to T160A mutation a key determinant dual receptor binding property H5NX subtypes. Our present study aims explore other effects this site among viruses. Here we report N-linked...
ABSTRACT Most highly pathogenic avian influenza A viruses cause only mild clinical signs in ducks, serving as an important natural reservoir of viruses. However, we isolated two H5N1 that are genetically similar but differ greatly virulence ducks. A/Chicken/Jiangsu/k0402/2010 (CK10) is pathogenic, whereas A/Goose/Jiangsu/k0403/2010 (GS10) low pathogenic. To determine the genetic basis for high CK10 generated a series single-gene reassortants between and GS10 tested their Expression PA or...
Newcastle disease (ND) leading to heavy economic losses the poultry industry worldwide is caused by virus (NDV). Even though intensive vaccination programs have been implemented in many countries, virulent NDV can still be frequently isolated well-vaccinated flocks. We compared protection efficiency of LaSota and two sub-genotype VIId vaccines, NDV/AI4 O/AI4, which O/AI4 was constructed replacing hemagglutinin–neuraminidase (HN) gene vaccine strain with that from variant JS-14-12-Ch cross...
Clade 2.3.4.4 H5N6 avian influenza virus (AIV) has been predominant in poultry China, and the circulating haemagglutinin (HA) gene changed from clade 2.3.4.4h to 2.3.4.4b recent years. In 2021, we isolated four viruses ducks during routine surveillance of AIV China. The whole-genome sequencing results demonstrated that isolates all belonged currently prevalent but had different internal constellations, which could be divided into G1 G2 genotypes. Specifically, possessed H9-like PB2 PB1 genes...