A5100359502- Pluripotent Stem Cells Research
- CRISPR and Genetic Engineering
- RNA modifications and cancer
- Cholangiocarcinoma and Gallbladder Cancer Studies
- Pancreatic function and diabetes
- Cancer-related gene regulation
- Epigenetics and DNA Methylation
- Reproductive Biology and Fertility
- Autophagy in Disease and Therapy
- 3D Printing in Biomedical Research
- Tissue Engineering and Regenerative Medicine
- Microbial Community Ecology and Physiology
- Protist diversity and phylogeny
- Muscle Physiology and Disorders
- Calcium signaling and nucleotide metabolism
- RNA Research and Splicing
- Wnt/β-catenin signaling in development and cancer
- Cancer-related molecular mechanisms research
- Hippo pathway signaling and YAP/TAZ
- Traditional Chinese Medicine Analysis
- Natural product bioactivities and synthesis
- Metabolomics and Mass Spectrometry Studies
- Cancer, Stress, Anesthesia, and Immune Response
- Methane Hydrates and Related Phenomena
- Renal and related cancers
University of Macau
2014-2024
Guangxi University
2024
Agricultural Genomics Institute at Shenzhen
2024
Chinese Academy of Agricultural Sciences
2021-2024
The First People’s Hospital of Lianyungang
2024
Shenyang Pharmaceutical University
2013-2024
Shenyang University of Technology
2024
Chongqing Maternal and Child Health Hospital
2020-2023
Chongqing Medical University
2022-2023
South China Sea Institute Of Oceanology
2023
Patients with ulcerative colitis (UC) have a high prevalence of mental disorders, such as depression and anxiety. Gut microbiota imbalance disturbed metabolism been suggested to play an important role in either UC or disorders. However, little is known about their detailed multi-omics characteristics patients depression/anxiety. In this prospective observational study, 240 Chinese were enrolled, including 129 active (69 Phase 1 60 2; divided into depression/non-depression anxiety/non-anxiety...
Lacking a self-contained metabolism network, viruses have evolved multiple mechanisms for rewiring the metabolic system of their host to hijack host's resources replication. Newcastle disease virus (NDV) is paramyxovirus, as an oncolytic currently being developed cancer treatment. However, how NDV alters cellular still far from fully understood. In this study, we show that infection reprograms cell by increasing glucose utilization in glycolytic pathway. Mechanistically, induces...
Nicotinamide, the amide form of vitamin B3, is widely used in disease treatments and stem cell applications. However, nicotinamide's impact often cannot be attributed to its nutritional functions. In a screen, we find that nicotinamide promotes survival differentiation human pluripotent cells. Nicotinamide inhibits phosphorylation myosin light chain, suppresses actomyosin contraction, leads improved after individualization. Further analysis demonstrates an inhibitor multiple kinases,...
Physalin A (1) is a withanolide isolated from Physalis alkekengi var. franchetii. In this study, the selective growth inhibitory effects on tumor cells induced by 1 were screened, and mechanism was investigated 1-induced inhibition, including apoptosis autophagy, in human fibrosarcoma HT1080 cells. Apoptosis associated with up-regulation of caspase-3 caspase-8 expression. However, there no significant changes caspase-9, Bid, Bax, Bcl-2 expression, indicating that occurs mainly through...
Oxidative stress plays an important role in the pathogenesis of cardiovascular diseases. Salidroside (SAL), one main effective constituents Rhodiola rosea, has been reported to suppress oxidative stress-induced cardiomyocyte injury and necrosis by promoting transcription nuclear factor E2-related 2 (Nrf2)-regulated genes such as heme oxygenase-1 (HO-1) NAD(P)H dehydrogenase (quinone1) (NQO1). However, it not indicated whether SAL might ameliorate endothelial induced stress. Here, our study...
Newcastle disease virus (NDV), a member of the Paramyxoviridae family, can activate PKR/eIF2α signaling cascade to shutoff host and facilitate viral mRNA translation during infection, however, mechanism remains unclear. In this study, we revealed that NDV infection up-regulated cap-dependent machinery by activating PI3K/Akt/mTOR p38 MAPK/Mnk1 pathways. addition, induced participated 4E-BP1 hyperphosphorylation for efficient protein synthesis when mTOR is inhibited. Furthermore, NP was found...
Abstract Endothelial barrier (EB) breaching is a frequent event during inflammation, and it followed by the rapid recovery of microvascular integrity. The molecular mechanisms EB are poorly understood. Triggering MHC molecules migrating T-cells minimal signal capable inducing endothelial contraction transient leakage. Using this model, we show that requires CD31 receptor-induced, robust glycolytic response sustaining junction re-annealing. Mechanistically, involves src-homology phosphatase...
ABSTRACT Oligotrichs are ciliates of great abundance, but their molecular systematics rarely studied. In this study, nine species representing three genera ( Strombidium , Novistrombidium and Omegastrombidium ) marine oligotrich were collected from coastal waters China. The small subunit (SSU) rRNA gene two the internal transcribed spacers 5.8S region (ITS1-5.8S-ITS2) for all sequenced first time. Phylogenetic trees using both SSU ITS1-5.8S-ITS2 sequences generated. addition, secondary...
Tribbles homolog 2 (TRIB2) is specifically regulated by Wnt signaling in liver cancer cells but not colon cells. However, whether and how TRIB2 regulates remains unclear. Here, we report that negatively activity through a reduction protein stability of TCF4 β‐Catenin. Mechanistically, associated‐ubiquitin E3 ligases beta‐transducin repeat‐containing ubiquitin ligase (β‐TrCP), COP1 Smad ubiquitination regulatory factor 1 (Smurf1) reduced TCF4/β‐Catenin expression, these effects could be...
Pyruvate is a key metabolite in glycolysis and the tricarboxylic acid (TCA) cycle. Exogenous pyruvate modulates metabolism, provides cellular protection, essential for maintenance of human preimplantation embryos embryonic stem cells (hESCs). However, little known about how contributes to cell-fate determination during epiblast stage. In this study, we used hESCs as model demonstrate that elevated exogenous shifts metabolic balance toward oxidative phosphorylation both differentiation...
Insulin is present in most maintenance media for human embryonic stem cells (hESCs), but little known about its essential role the cell survival of individualized during passage. In this article, we show that insulin suppresses caspase cleavage and apoptosis after dissociation. activates insulin-like growth factor (IGF) receptor PI3K/AKT cascade to promote function independent rho-associated protein kinase regulation. During niche reformation passaging, integrin survival. IGF colocalizes...
Insulin is an essential growth factor for the survival and self-renewal of human embryonic stem cells (hESCs). Although it best known as principal hormone promoting glycolysis in somatic cells, insulin's roles hESC energy metabolism remain unclear. In this report, we demonstrate that insulin to sustain mitochondrial respiration rapidly decreased upon removal. Insulin-dependent cell specific, mainly relies on pyruvate glutamine, while glucose suppresses excessive oxidative phosphorylation....
Abstract Bromodomain-containing protein 9 (BRD9) is a specific subunit of the non-canonical SWI/SNF (ncBAF) chromatin-remodeling complex, whose function in human embryonic stem cells (hESCs) remains unclear. Here, we demonstrate that impaired BRD9 reduces self-renewal capacity hESCs and alters their differentiation potential. Specifically, depletion inhibits meso-endoderm while promoting neural ectoderm differentiation. Notably, supplementation NODAL, TGF-β, Activin A or WNT3A rescues...
Cell density has profound impacts on the cell culture practices of human pluripotent stem cells.The regulation growth, death, pluripotency and differentiation converge at high density, but it is largely unknown how different regulatory mechanisms act this stage.We use a chemically defined medium to systemically examine cellular activities impact components in high-density culture.We show that acidosis main factor alters cycle, gene expression metabolism density.The low pH leads inhibition...