Abstract Splicing factors are key players in the regulation of alternative splicing pre-mRNAs. Overexpression factors, including SRSF3, has been strongly linked with oncogenesis. However, mechanisms behind their overexpression remain largely unclear. Autoregulation is a common mechanism to maintain relative stable expression levels cells. SRSF3 regulates its own by enhancing inclusion an exon 4 in-frame stop codon. We found that impaired oral squamous cell carcinoma (OSCC) PTBP1 and PTBP2...

Objective: Oral squamous cell carcinoma (OSCC) is the most common malignancy of head and neck with high mortality rates.The mechanisms initiation development OSCC remain largely unknown.Dysregulated alternative splicing pre-mRNA has been associated OSCC.Splicing factor SRSF3 a proto-oncogene overexpressed in multiple cancers.The aim this study was to uncover relationship between carcinogenesis progression oral carcinoma.Design Methods: The expression normal, dysplasia, or tissues analyzed by...

Oral squamous cell carcinoma (OSCC) is a common human malignant tumor with high mortality. So far, the molecular pathogenesis of OSCC remains largely unclear. Heterogeneous nuclear ribonucleoprotein (hnRNP) A1 an important multi‐function splicing factor and closely related to tumorigenesis. hnRNP overexpressed in various tumors, promotes aerobic glycolysis elongation telomere, but function cycle proliferation We found that was tissues, required for growth cells. Moreover, highly expressed...

High risk forms of the human papilloma virus (HPV) are generally accepted as necessary causative agents for cervical cancer. Recently, a possible relation between HPV and oral squamous cell carcinoma (OSCC) has also been noticed. The present study was conducted to investigate prevalence infection in OSCCs Wuhan city. DNA samples were collected from fresh tissues 200 patients with OSCC 68 normal controls. polymerase chain reaction direct sequencing used identify types samples. all group...

STAT3 plays very important roles in the initiation and development of tumors.Despite extensive studies repressing its activation function via multiple ways, so far, there are few effective therapeutic methods to inhibit clinic.STAT3 has two isoforms generated by alternative splicing exon 23.STAT3α is longer isoform encodes full-length oncogenic STAT3α protein.STAT3β shorter truncated tumor-suppressive STAT3β protein.It remains unknown how 23 regulated.Here, we discovered that an exonic...

Abstract Oral squamous cell carcinoma (OSCC) is the leading cause of death related to oral diseases. The mechanisms OSCC development remain largely unknown. Heterogeneous nuclear ribonucleoprotein L (HnRNP L) a multi-functional splicing factor. It has been reported be an important regulator apoptosis. However, functions hnRNP in cancer need further explored. In present study, we found that tissues expressed significantly higher levels than normal tissues. Depletion retarded growth,...

Extracellular vesicles, as bioactive molecules, have been extensively studied. There are abundant studies in the literature on their biogenesis, secretion, structure, and content, roles pathophysiological processes. vesicles reviewed biomarkers for use diagnostic tools. Saliva contains many extracellular compared with other body fluids, it is easier to obtain a non-invasive way, making its acquisition more easily accepted by patients. In recent years, there numerous new investigating role of...

Glucosyltransferases (GTFs) and A cell-surface protein (PAc) are two important virulence factors of the cariogenic organism Streptococcus mutans. They may mediate sucrose-independent or sucrose-dependent attachment mutans to tooth surfaces, respectively. Thus, inhibiting both is predicted provide better protection against caries than a single factor. To develop highly efficient vaccine caries, we constructed fusion DNA vaccine, pGLUA-P, by cloning GLU region GTF into pCIA-P, which encodes...

Preventive anti-HPV vaccines are effective against HPV infection but not existing HPV-associated diseases, including cervical cancer and other malignant diseases. Therefore, the development of therapeutic is urgently needed. To improve anti-tumor effects vaccine, we fused cytotoxic T-lymphocyte antigen 4 (CTLA-4) with HPV16 E7 E6 as a fusion DNA vaccine (pCTLA4-E7E6). pCTLA4-E7E6 induced significantly higher anti-E7E6 specific antibodies relatively stronger CTL responses than nonfusion pE7E6...

Inhibition of the expression oncogene SRSF3 and cancer cell growth by blocking an exonic splicing suppressor in alternative exon 4 with SR-3 antisense oligonucleotide.

Mutans streptococci (MS) are generally considered to be the principal etiological agent of dental caries. MS have two important virulence factors: cell- surface protein PAc and glucosyltransferases (GTFs). GTFs functional domains: an N-terminal catalytic sucrose-binding domain (CAT) a C-terminal glucan-binding (GLU). A fusion anti-caries DNA vaccine, pGJA-P/VAX, encoding antigenic domains, GLU, S. mutans, was successful in reducing levels caries caused by mutans gnotobiotic animals. However,...

Ki67 is a well-known proliferation marker with large size of around 350 kDa, but its biological function remains largely unknown. The roles in tumor prognosis are still controversial. has two isoforms generated by alternative splicing exon 7. and regulatory mechanisms progression not clear. In the present study, we surprisingly find that increased inclusion 7, total expression level, was significantly associated poor multiple cancer types, including head neck squamous cell carcinoma (HNSCC)....

The interaction between programmed cell death 1 ligand (PD-L1) and protein (PD-1) protects tumor cells from immune surveillance. PD-L1 exon 3 is a potential alternative encodes an Ig variable (IgV) domain. Here, we found that lack of leads to the significant loss cellular membrane locations dramatically reduced expression PD-L1, indicating essential for its translocation membrane. Notably, oral cancer show almost no skipping ensure full-length, functional protein. We discovered two key...