A5028939266- Ubiquitin and proteasome pathways
- Viral Infections and Vectors
- Mosquito-borne diseases and control
- Cancer-related Molecular Pathways
- Protein Degradation and Inhibitors
- Malaria Research and Control
- Microtubule and mitosis dynamics
- Immunotherapy and Immune Responses
- T-cell and B-cell Immunology
- CRISPR and Genetic Engineering
- HIV/AIDS drug development and treatment
- Peptidase Inhibition and Analysis
- Autophagy in Disease and Therapy
- Medical Imaging and Pathology Studies
- vaccines and immunoinformatics approaches
- CAR-T cell therapy research
- Viral Infections and Outbreaks Research
- Plant Pathogens and Fungal Diseases
- HIV Research and Treatment
- Protein purification and stability
- Plant Virus Research Studies
- Sirtuins and Resveratrol in Medicine
- Immune Response and Inflammation
- Viral gastroenteritis research and epidemiology
- Robotic Locomotion and Control
Guilin Medical University
2021
University of California, Los Angeles
2007-2020
Newcastle University
2011-2016
Shanghai Medical College of Fudan University
2015
Fudan University
2015
Tokai University
2015
Takeda (Japan)
2014
Millennium Engineering and Integration (United States)
2010-2013
Takeda (United States)
2010-2013
Wuhan University
2006-2009
Previous studies have demonstrated that monoclonal antibodies (MAbs) against an epitope on the lateral surface of domain III (DIII) West Nile virus (WNV) envelope (E) strongly protect infection in animals. Herein, we observed significantly less efficient neutralization by 89 MAbs recognized I (DI) or II (DII) WNV E protein. Moreover, cells expressing Fc gamma receptors, many DI- and DII-specific enhanced over a broad range concentrations. Using yeast display protein variants, identified 25...
Background. West Nile virus (WNV) is a mosquitoborne flavivirus that can cause severe meningitis and encephalitis in infected individuals. We report the safety immunogenicity of WNV DNA vaccine its first phase 1 human study. Methods. Asingle-plasmidDNAvaccine encoding premembrane envelope glycoproteins NY99 strain was evaluated an open-label study 15 healthy adults. Twelve subjects completed 3-dose vaccination schedule, all 32 weeks evaluation for immunogenicity. The development...
ABSTRACT Previous studies have established that an epitope on the lateral ridge of domain III (DIII-lr) West Nile virus (WNV) envelope (E) protein is recognized by strongly neutralizing type-specific antibodies. In contrast, against fusion loop in II (DII-fl) flavivirus cross-reactive antibodies with less potential. Using gain- and loss-of-function E proteins wild-type variant WNV reporter particles, we evaluated expression pattern activity DIII-lr DII-fl epitopes mouse human serum after...
Inhibition of the MDM2-p53 interaction has been shown to produce an antitumor effect, especially in MDM2 amplified tumors. The isoindolinone scaffold proved be versatile for discovery antagonists. Optimization previously reported inhibitors, example, NU8231 (7) and NU8165 (49), was guided by NMR titrations, which indicated key areas binding explored. Variation 2-N-benzyl 3-alkoxy substituents resulted identification...
Ubiquitin-activating enzyme (UAE or E1) activates ubiquitin via an adenylate intermediate and catalyzes its transfer to a ubiquitin-conjugating (E2). MLN4924 is adenosine sulfamate analogue that was identified as selective, mechanism-based inhibitor of NEDD8-activating (NAE), another E1 enzyme, by forming NEDD8-MLN4924 adduct tightly binds at the active site NAE, novel mechanism termed substrate-assisted inhibition (Brownell, J. E., Sintchak, M. D., Gavin, M., Liao, H., Bruzzese, F. J.,...
There is a desperate need for effective therapies to fight chronic viral infections. The immune response normally fastidious at controlling the majority of infections and therapeutic strategy aimed reestablishing control represents potentially powerful approach towards treating persistent We examined potential genetically programming human hematopoietic stem cells generate mature CD8+ cytotoxic T lymphocytes that express molecularly cloned, "transgenic" anti-HIV cell receptor (TCR). Anti-HIV...
Non-genotoxic reactivation of the p53 pathway by MDM2-p53 binding antagonists is an attractive treatment strategy for wild-type TP53 cancers. To determine how resistance to MDM2/p53 might develop, SJSA-1 and NGP cells were exposed growth inhibitory concentrations chemically distinct MDM2 inhibitors, Nutlin-3 MI-63, clonal resistant cell lines generated. The mediated responses parental compared. In contrast lines, activation Nutlin-3, MI-63 or ionizing radiation was not observed in either...
Abstract COVID-19 pneumonia has now spread widely in the world. Currently, no specific antiviral drugs are available. The vaccine is most effective way to control epidemic. Passive immune antibodies also an method prevent and cure pneumonia. We used SARS-CoV-2 S receptor-binding domain (RBD) as antigen immunize layers order extract, separate, purify SARS-CoV-2-IgY from egg yolk. (S-IgY)can block entry of into Cells reduce viral load cells. Half concentration (EC 50 ) W3-IgY (S-IgY third week...
Cryptosporidiosis has been reported to be associated with HIV/acquired immune deficiency syndrome, which greatly reduces the quality of life and shortens expectancy HIV-infected patients. In order properly treat infected patients, accurate automatic diagnostic tools need developed. this study, a novel microfluidic immunochip system was presented for surveillance rapid detection Cryptosporidium infection in 190 patients from Guangxi, China, using P23 antigen Cryptosporidium. The procedure can...
Fusing antigens to cytotoxic T-lymphocyte antigen 4 (CTLA-4) represents an effective approach enhance DNA vaccine efficacy. It has been speculated that the direct targeting of CTLA-4 fusion antigen-presenting cells (APCs) causes be processed and presented T more efficiently, leading a stronger immune response. In present study, dendritic (DCs), most potent APCs, were generated from human monocytes. The specific binding protein DCs was investigated by flow cytometry. results showed capable B7...
Here we demonstrate the mechanical robustness of microfabricated torsional magnetic actuators in withstanding fields produced by a 7 T MRI magnet. The static and dynamic characteristics 30 devices were quantitatively measured before after exposure to both uniform non-uniform fields. results show no statistically significant change performance, which mitigates concerns about compatibility our with scanners. Additional experiments are required quantify potential image-artifact size...