A5101926996- Animal Virus Infections Studies
- Virus-based gene therapy research
- Viral gastroenteritis research and epidemiology
- interferon and immune responses
- Cancer-related Molecular Pathways
- Influenza Virus Research Studies
- Catalytic Cross-Coupling Reactions
- Catalytic C–H Functionalization Methods
- Advanced Condensed Matter Physics
- Immune Response and Inflammation
- Physics of Superconductivity and Magnetism
- Advanced Photocatalysis Techniques
- Immunotherapy and Immune Responses
- Bacillus and Francisella bacterial research
- Acupuncture Treatment Research Studies
- Cytokine Signaling Pathways and Interactions
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Coal Properties and Utilization
- Hydrocarbon exploration and reservoir analysis
- Advanced Materials Characterization Techniques
- Asymmetric Synthesis and Catalysis
- Diabetes and associated disorders
- Powder Metallurgy Techniques and Materials
- Polyoxometalates: Synthesis and Applications
- High-pressure geophysics and materials
Shanghai Veterinary Research Institute
2008-2022
Chinese Academy of Agricultural Sciences
2012-2022
Jiangsu University of Science and Technology
2020-2022
University of Tennessee Health Science Center
2016
CPC Heilongjiang Provincial Committee Party School
2015
Dingxi City People's Hospital
2014
Dawu County People's Hospital
2013
Chinese Academy of Agricultural Engineering
2013
Argonne National Laboratory
1991-1992
ABSTRACT Accumulating data suggest that tripartite-motif-containing (TRIM) proteins participate in host responses to viral infections, either by acting as direct antiviral restriction factors or through regulating innate immune signaling of the host. Of >70 TRIMs, TRIM56 is a factor several positive-strand RNA viruses, including three members family Flaviviridae (yellow fever virus, dengue and bovine diarrhea virus) human coronavirus (OC43), this ability invariably depends upon E3 ligase...
Newcastle disease virus (NDV), a member of the Paramyxoviridae family, can activate PKR/eIF2α signaling cascade to shutoff host and facilitate viral mRNA translation during infection, however, mechanism remains unclear. In this study, we revealed that NDV infection up-regulated cap-dependent machinery by activating PI3K/Akt/mTOR p38 MAPK/Mnk1 pathways. addition, induced participated 4E-BP1 hyperphosphorylation for efficient protein synthesis when mTOR is inhibited. Furthermore, NP was found...
Abstract MicroRNAs (miRNAs) can impact viral infections by binding to sequences with partial complementarity on RNA transcripts, usually resulting in the repression of virus replication. In present study, we identified a potential site for miR-130 5′ untranslated region (bps 155-162) porcine reproductive and respiratory syndrome (PRRSV) genome. We found that delivery multiple family mimics, especially miR-130b, resulted inhibition PRRSV replication vitro . was effective inhibiting type 2...
The green synthesis of (hetero)aromatic amines is highly expected but challenging.
Marek's disease virus (MDV) is an oncogenic herpesvirus, which causes malignant lymphoma in chickens. The Meq protein of MDV, expressed abundantly MDV-infected cells and (MD) tumor cells, functions as a transcriptional activator has been proposed to play important role transformation. Preliminary studies demonstrated that able bind p53 vitro, using protein-binding assay. This observation prompted us examine whether the interaction between occurs investigate biological significance this...
Porcine epidemic diarrhea virus (PEDV) has recently caused high mortality in suckling piglets with subsequent large economic losses to the swine industry. Many intracellular signaling pathways, including phosphatidylinositol 3-kinase (PI3K)/Akt pathway, are activated by viral infection. The PI3K/Akt pathway is an important cellular that been shown be required for replication. In present study, we found PEDV JS-2013 strain Akt Vero cells at early (5-15 min) and late stages (8-10 h) of...
The regio- and chemoselective construction of indole bearing an all-carbon center at the C3-position, a versatile bioactive building block, by C(sp2)–C(sp3) formation with olefins has been achieved through utilization hexafluoroisopropanol (HFIP) as protonation reagent solvent. catalytic reactions are operationally simple green compared previous reports utilizing elaborated catalysts. This protocol allows for alkylation variety substituted indoles diverse styrene type alkenes in excellent...
Abstract Porcine epidemic diarrhea virus (PEDV) is a highly contagious coronavirus that causes severe and death, particularly in neonatal piglets. The nucleocapsid protein (N protein) of PEDV presents strong immunogenicity contributes to the cross-reactivity between TGEV. However, characterization epitopes on N remains largely unknown. Here, two monoclonal antibodies (MAbs) specific strain, FJzz1/2011, were generated screened against partially overlapping library 24 GST-fusion...
Abstract SAMHD1 is a type I interferon (IFN) inducible host innate immunity restriction factor that inhibits an early step of the viral life cycle. The underlying mechanisms transcriptional regulation remains elusive. Here, we report inducing upregulation part intrinsic immune response via TLR3 and RIG-I/MDA5 agonists ultimately induce nuclear translocation 3 (IRF3) protein. Further studies show IRF3 plays major role in upregulating endogenous expression mechanism independent classical...
Herein, we have developed an efficient tungsten-catalyzed homogeneous system for oxidative dehydrogenative coupling of anilines to selectively produce various azoaromatics and azoxyaromatics as well 2-substituted indolone N-oxides by simply regulating the reaction solvent with peroxide a terminal oxidant under additive-free conditions. These findings provide experimental framework exploring tungsten catalysis in organic synthesis offer convenient tactic selective oxidation anilines.
Triggering C-N bond formation with nitroaromatics and boronic acids at mild conditions is highly desirable, since most prior works were carried out under harsh sometimes suffered from poor chemo- or regioselectivity. Herein, a low-valent-tungsten-catalyzed reaction that enables the ambient temperature amination of disclosed. With readily available W(CO)6 as precatalyst external-photosensitizer-free conditions, smoothly undergo coupling reactions their acid partners, delivering structurally...
Abstract The construction of C−N bonds with nitroaromatics and boronic acids using highly efficient recyclable catalysts remains a challenge. In this study, nanoporous MoO 3 confined in silica serves as an heterogeneous catalyst for cross‐coupling aryl or alkyl to deliver N ‐arylamines desirable multiple reusability. Experimental results suggest that not only heterogenizes the Mo species mesoporous microenvironment but also significantly reduces reaction induction period regulates chemical...
The N gene of porcine epidemic diarrhea virus (PEDV) was amplified by RT-PCR using specific primers, and inserted into the expression vector pCold-I to construct a recombinant plasmid pCold-I-N. expressed in Escherichia coli BL21 (DE3) under IPTG induction. Then, female BALB/c mice were immunized with purified protein one strain hybridoma cells named 2B8 secreting anti-N monoclonal antibodies (MAb) obtained technique. MAb specifically reacted PEDV identified Western blot indirect...
Highly pathogenic porcine reproductive and respiratory syndrome (HP-PRRS), which emerged in China 2006, was characterized by high fever, morbidity mortality. The causative agent of the disease a highly variant virus (also called HP-PRRSV), has discontinuous deletion 1 + 29 amino acids (aa) Nsp2 coding region, compared to classical PRRSV. In 2014, fattened pigs on pig farm Jiangxi Province suffered from clinical symptoms dyspnoea death. A PRRSV, termed JX2014T2, isolated samples dead pigs....
Astroviruses have been widely described in mammalian and avian species. Here, we report a complete genome sequence of novel porcine astrovirus (PoAstV) isolated from fecal sample China. The consists 6,611 nucleotides, excluding the 3' poly(A) tail, has two open reading frames (ORFs). ORF1 maps between nucleotide positions 19 4211 encodes 1,396-amino-acid (aa) polyprotein precursor consisting nonstructural protein putative RNA-dependent RNA polymerase, ORF2 4202 6531 775-aa which is capsid...