- Reproductive Biology and Fertility
- Ovarian function and disorders
- Sperm and Testicular Function
- Reproductive Physiology in Livestock
- Genetic and phenotypic traits in livestock
- Chemotherapy-induced organ toxicity mitigation
- Endometriosis Research and Treatment
- Regulation of Appetite and Obesity
- Rabbits: Nutrition, Reproduction, Health
- Circadian rhythm and melatonin
- Adipose Tissue and Metabolism
- Sirtuins and Resveratrol in Medicine
- Agroforestry and silvopastoral systems
- FOXO transcription factor regulation
- Moringa oleifera research and applications
- Tissue Engineering and Regenerative Medicine
- Agronomic Practices and Intercropping Systems
- Renal and related cancers
- Organic and Inorganic Chemical Reactions
- Cannabis and Cannabinoid Research
- Ruminant Nutrition and Digestive Physiology
- Natural Compound Pharmacology Studies
- Genomics, phytochemicals, and oxidative stress
- Tea Polyphenols and Effects
- Dietary Effects on Health
Universidade Federal do Vale do São Francisco
2011-2024
Universidade São Francisco
2015-2019
We investigated the effects of insulin-like growth factor 1 (IGF-1) on morphology and follicular activation ovine preantral follicles cultured in situ whether phosphatidylinositol 3-kinase/protein kinase B (PI3K/AKT) pathway is involved IGF-1 action sheep ovary. Ovine ovarian fragments were fixed for histological terminal deoxynucleotidyl transferase dUTP nick-end labelling (TUNEL) analyses (fresh control) or supplemented alpha-minimum essential medium (α-MEM+; α-MEM+ with (1, 10, 50, 100...
This study evaluated the potential of Cimicifuga racemosa (L.) Nutt extract (CIMI) to reduce deleterious effects doxorubicin (DOXO) in oocytes, follicles and stromal cells mice ovaries cultured vitro. In experiment 1, were DMEM+ alone or supplemented with 5, 50 500 ng/mL CIMI, while 2, 5 CIMI (better concentration), 0.3 μg/mL DOXO both. Thereafter, processed for histological (morphology, growth, activation, extracellular matrix configuration cell density), immunohistochemical (caspase-3)...
We aimed to study the effects of resveratrol on morphology, DNA fragmentation, follicular activation and cell proliferation after in vitro culture ovine ovarian tissue, verify if PI3K pathway is involved action sheep ovary. Ovaries were collected divided into fragments. One fragment was fixed for histology (fresh control). The remaining fragments cultured 7 days control medium (α-MEM+ ) alone or with (2, 10 30 µM). After culture, tissue destined morphological analysis. TUNEL proliferating...
Summary The present study aimed to investigate the effect of quercetin as an alternative antioxidant cysteamine on in vitro maturation. Oocytes were collected from goat ovaries, destined for maturation and distributed into three groups: CIS group, oocytes immersed MIV base medium; Groups Q4 Q8, medium adding 4 μM or 8 quercetin, respectively, cultured 24 h at 38.5°C with 5% CO 2 . groups presented same percentage expanded cumulus cells, but per cent Q8 group was significantly lower than that...
Summary The worldwide consumption of red wine, nuts and grapes has resulted in increased human exposure to resveratrol, which could affect reproductive function. However, the effect resveratrol on vitro culture early-stage ovarian follicles never been investigated. aims present study were evaluate sheep secondary follicle morphology, growth, DNA fragmentation, intracellular levels glutathione (GSH) active mitochondria. Secondary isolated from ovaries cultured for 18 days supplemented α -MEM...
Abstract This study characterized the expression of melatonin receptor type 1 (MT ) protein in sheep ovaries, evaluated effects on primordial follicle survival and development after vitro culture ovarian tissue verified possible involvement phosphatidylinositol‐3‐kinase/protein kinase B/forkhead box O3a (PI3K/Akt/FOXO3a) pathway actions. Ovine fragments were cultured α‐modified minimum essential medium alone (α‐MEM + or supplemented with 100, 500, 1000 pg/ml for 7 days. PI3K inhibition was...
The objective of this study was to evaluate the effectiveness reusing a controlled internal drug release (CIDR) device for up three times in reproductive performance dairy goats raised semi-arid zone northeastern Brazil. Forty-five were allocated into hormone treatments, as follows: CIDR1x, treated with new CIDR during nine days. Two days prior removal, injections 75 µg d-cloprostenol and 300 IU equine chorionic gonadotropin (eCG) administrated. For other same protocol used, differing only...
<p>To evaluate the effect of pFSH dose on <em>in vivo </em>embryo production Dorper ewes in semi-arid northeast Brazil, 40 sheep females were distributed into two groups 20 animals that received intravaginal CIDR for 14 days, and days before device removal, they one following treatments: FSH200 group, 200 mg pFSH; FSH128 a total 128 decreasing doses every 12 h. Beginning h after conclusion treatments, estrus detection was performed four hours using rams proven fertility....
This study aimed to characterize the hydroethanolic extract of red propolis (HERP) and nanoparticles containing HERP for using as an additive in culture medium isolated ovine preantral follicles. was characterized by high-performance liquid chromatography (HPLC) determination flavonoid content, mean particle diameter, polydispersity index (PI) encapsulation efficiency (EE). The effect (10 20 ηg/ml-HERP10 HERP20 groups) (NP10 NP20 on secondary follicles cultured vitro 12 days observed...