A5029169486- Reproductive Biology and Fertility
- Sperm and Testicular Function
- Reproductive Physiology in Livestock
- Animal Genetics and Reproduction
- Ovarian function and disorders
- Reproductive biology and impacts on aquatic species
- Genetic and phenotypic traits in livestock
- Regulation of Appetite and Obesity
- Effects of Environmental Stressors on Livestock
- Parasitic Infections and Diagnostics
- Bee Products Chemical Analysis
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Helminth infection and control
- Adipose Tissue and Metabolism
- Adipokines, Inflammation, and Metabolic Diseases
- Reproductive Health and Technologies
- Seed Germination and Physiology
- Toxoplasma gondii Research Studies
- Parasite Biology and Host Interactions
- Veterinary Equine Medical Research
- Herpesvirus Infections and Treatments
- Aquaculture Nutrition and Growth
- Coccidia and coccidiosis research
- Selenium in Biological Systems
- Aldose Reductase and Taurine
Universidade Federal Rural de Pernambuco
2015-2024
Universidade Federal de Pernambuco
2011-2012
State University of Norte Fluminense
2011-2012
Contents The aim of the present study was to evaluate in vitro and vivo effect addition superoxide dismutase (SOD) reduced glutathione (GSH) ram semen freezing extender. Significant differences (p < 0.05) were detected between groups regarding total motility (TM), straightness (STR) wobble (WOB), for which GSH 7 m group had lesser TM better STR than other 5 higher values control, SOD 25 100 U/ml groups. ultrastructural analysis revealed that acrosome preserved after 2 groups, whereas...
The aim of this study was to evaluate the effect different concentrations soybean lecithin (SL) in extenders for sperm goat cryopreservation. Sexually mature male Saanen goats (n = 4) were used, and ejaculates obtained using an artificial vagina method. semen samples pooled diluted a skim milk-based extender (control group; CG) or Tris supplemented with SL at (G1 0.04%, G2 0.08% G3 0.16%) final concentration 240 × 106 spermatozoa/mL. packed straws (0.25 mL), frozen automated system stored...
Neoplastic transformation is the abnormal proliferation of cells. These transformations are often related to changes in cell surface glycoconjugates which can be detected by lectins. We evaluated anti-tumor potential BlL, a galactoside-binding lectin isolated from Bothrops leucurus venom as well its cytotoxicity and hemolysis activity. The phosphatidylserine externalization mitochondrial membrane were also determined. BlL exhibited cytotoxic activity against all tumor lines tested induced...
Toxoplasmosis is a parasitic disease caused by Toxoplasma gondii that affects reproductive performance in small ruminants. Although the T. life cycle well understood since 1960s, several aspects related to its infection remain unclear. In present study we hypothesized sheep inseminated with gondii-contaminated semen would develop toxoplasmosis. order test hypothesis, 41 were experimentally infected spiked organism. Females divided three groups (G1-G3): (a) females G1 group containing 6.5 x...
In the study, we have examined antitumor and antimicrobial activities of methanol extract, fractions, a fraction total alkaloids two isolated from stem Erythroxylum caatingae Plowman. All test except hexane showed activity on gram-positive bacteria fungi. The acetate: (95:5), acetate, chloroform fractions show highest cytotoxicity against NCI-H292, HEp-2 K562 cell lines using MTT. absence hemolysis in erythrocytes mice was observed these 6β-Benzoyloxy-3α-(3,4,5- trimethoxybenzoyloxy) tropane...
The addition of antioxidants to semen cryopreservation extenders has been employed for combating oxidative damage. This work aimed evaluate the carotenoid canthaxanthin a extender ram semen. Three breeder rams were used and, after collection, with 48-hour intervals between samples included in pool formation (n = 6). experimental groups comprised 0 (control), 0.1, 1, 10, and 25 μM canthaxanthin. After thawing (37°C/30 s) incubation at 37°C 2 hours, aliquots from each group evaluated sperm...
The objective of this study was to investigate the need seminal plasma removal for short-term cooling buck semen in soybean lecithin (SL) based extender.Each pool divided equally, and one half subjected centrifugation remove (SP-), while other remained with (SP+).Then, both SP+ SP-samples were diluted two SL extenders (extender A = 1% SL; extender B 2% SL), cooled 5ºC stored 48 hours.The sperm kinetics, evaluated by CASA, membrane integrity (PMI), acrosomal (ACI) high mitochondrial potential...
O objetivo deste trabalho foi estudar a eliminação de Toxoplasma gondii no sêmen carneiros naturalmente infectados. Foram utilizados 65 reprodutores submetidos inicialmente à pesquisa anticorpos anti-T. por meio da técnica imunofluorescência indireta (IFI). Os sorologicamente positivos foram colheita para detecção do DNA T. gondii. Na sorologia observaram-se 6/65 (9,2%) positivos, enquanto PCR nested 4/6 (66,6%) positivos. Conclui-se que detecção, nested, forma proliferativa infectados,...
The aim of this work was to evaluate the in vitro effect adding Trolox freezing extender for goat semen. Ejaculates from five bucks were evaluated, and when approved, samples pooled, diluted according experimental groups [Trolox 0 (control), 30, 60 120 nmol ml−1] frozen an automated system. Thawed (37 °C/30 s) evaluated plasma membrane (PMi) acrosome integrity (Aci), mitochondrial potential (MMP) sperm kinematics by CASA Spermatozoa ultrastructure fresh post-thawed No significant difference...
l-Carnitine (LC) plays a key role in sperm metabolism, easily providing energy through β-oxidation, which positively affects motility. The objective of this study was to investigate the association between blood plasma and seminal LC levels, as well effect an additive skimmed milk-based extender during storage at 5°C. In first experiment, semen samples from 14 Quarter Horse stallions were used. content determined by spectrophotometry their relationships with parameters evaluated. second...
Contents Recent studies have shown that adiponectin, an adipokine predominantly produced by adipose tissue, regulates several reproductive processes. However, the mechanisms of action adiponectin on maturation goat oocytes remain to be determined. The aim this study was investigate whether (a) influences meiotic oocytes; (b) MAPK MEK 1/2 mediates effects adiponectin; and 3) differentially affects mRNA relative abundance genes relevant for signal transduction in oocytes. addition (5 μg/ml)...
This work aimed to evaluate the effect of stabilisation times, glycerol concentration, and catalase superoxide dismutase supplementation diluent on parameters frozen-thawed spermatozoa from epididymis Nelore bulls: Experiment 1: diluted in Tris-egg yolk with (3%, 5% or 7%) times (0, 2 4 hr at 5°C); 2: only, (CAT, 50 100 U ml−1) (SOD, ml−1). Frozen-thawed were evaluated for kinetic parameters, plasma membrane acrosome integrity, mitochondrial activity IVF capacity. ALH BCF affected (p < .05)...
The objective of this study was to investigate the effect melatonin supplement in different extenders on quality cryopreserved goat semen. Six semen pools were skim milk (SM – Experiment 1) or tris-egg yolk (TEY 2) based supplemented with (0, 0.5, 1, 2 and 4 mM). After thawing, kinetic sperm parameters [total motility (TM), progressive (PM), curvilinear velocity (VCL), straight-line (VSL), linearity (LIN), straightness (STR), wobble (WOB)] viability [plasma acrosomal membrane integrity...
Contents The aim of this study was to evaluate the effect leptin administration during superovulation on in vivo goat embryo production. Ten mature does were superovulated with 133 mg follicle‐stimulating hormone (FSH) i.m. six descending doses at 12‐h intervals. goats received 4.8 μg/kg human recombinant s.c. (leptin group, n = 5) or phosphate‐buffered saline (PBS) (control first and second FSH doses. mated subjected collection by transcervical technique 6 days later. total number cells per...