A5062687561- Reproductive Biology and Fertility
- Reproductive Physiology in Livestock
- Ovarian function and disorders
- Sperm and Testicular Function
- Reproductive System and Pregnancy
- Tissue Engineering and Regenerative Medicine
- Bioenergy crop production and management
- TGF-β signaling in diseases
- Sexual function and dysfunction studies
- Seaweed-derived Bioactive Compounds
- Renal and related cancers
- Seed Germination and Physiology
- Animal Genetics and Reproduction
- Marine Sponges and Natural Products
- Phytoestrogen effects and research
- Mosquito-borne diseases and control
- Male Reproductive Health Studies
- Phytochemistry and Bioactive Compounds
- Bioactive Compounds and Antitumor Agents
- Echinoderm biology and ecology
- Urinary Bladder and Prostate Research
- Parasite Biology and Host Interactions
- Occupational Health and Safety Research
- History, Culture, and Society
- Fibroblast Growth Factor Research
Universidade Federal de Pernambuco
2012-2024
Fundação Oswaldo Cruz
2013-2024
Universidade Tiradentes
2019
Centro de Tecnologias Estratégicas do Nordeste
2012
The actions of different concentrations insulin alone or in combination with follicle-stimulating hormone (FSH) were evaluated by vitro follicular development and mRNA expression cytochrome P450 aromatase (CYP19A1) as receptors for (INSR) FSH (FSHR) from isolated, cultured goat preantral follicles. Goat follicles microdissected 18 days the absence presence (5 10 ng/ml μg/ml) FSH. After days, addition maximum concentration to culture medium reduced survival antrum formation rates...
Summary This study investigated the effect of three different culture media (α minimum essential medium (α-MEM), McCoy or TCM199 during in vitro (IVC) bovine isolated pre-antral follicles. Pre-antral follicles greater than 150 μm size were and cultured for 0 (control), 8 16 days one abovementioned media. Follicles evaluated survival, growth antrum formation at 16. The results showed that was most suitable to preserve follicular viability ultrastructure, resulting highest rates formation. In...
This study evaluated the potential of Cimicifuga racemosa (L.) Nutt extract (CIMI) to reduce deleterious effects doxorubicin (DOXO) in oocytes, follicles and stromal cells mice ovaries cultured vitro. In experiment 1, were DMEM+ alone or supplemented with 5, 50 500 ng/mL CIMI, while 2, 5 CIMI (better concentration), 0.3 μg/mL DOXO both. Thereafter, processed for histological (morphology, growth, activation, extracellular matrix configuration cell density), immunohistochemical (caspase-3)...
The aim of this study was to evaluate the effects a dynamic medium containing kit ligand (KL) and follicle-stimulating hormone (FSH) on in vitro culture caprine preantral follicles for 16 days. Ovarian fragments were cultured α-MEM<sup>+</sup> or not KL (50 ng/ml) and/or FSH added during first (days 0–8) second half 8–16) period. Noncultured (control) processed histological ultrastructural evaluation. After 1 day culture, only treatments performed with maintained percentage...
Diethylcarbamazine (DEC) is an antifilarial drug with potent anti-inflammatory properties as a result of its interference the metabolism arachidonic acid. The aim present study was to evaluate activity DEC in mouse model acute inflammation (carrageenan-induced pleurisy). injection carrageenan into pleural cavity induced accumulation fluid containing large number polymorphonuclear cells (PMNs) well infiltration PMNs lung tissues and increased production nitrite tumor necrosis factor- α...
Abstract The objective of this study was to determine the effects TNF ‐α and IL ‐1β on development survival bovine secondary follicle culture in vitro for 18 days. Secondary follicles (~0.2 mm) were isolated from ovarian cortex individually cultured at 38.5°C, with 5% CO 2 air, days, TCM ‐199 + alone (cultured control) or supplemented 10 ng/ml ‐1β, both ‐1β. these treatments growth, follicular survival, antrum formation, viability, ultrastructure mRNA levels GDF ‐9, c‐ MOS , H1foo Cyclin B1...
This study evaluates the effect of different concentrations (0, 10, 50 and 100ng/mL) bone morphogenetic protein-2 (BMP-2) on primordial secondary follicle development. It also investigates effects FSH BMP-2 growth, morphology, ultrastructure expression mRNA for GDF9, NLRP5 NPM2 genes in follicles cultured 18 days. The presence at all tested increased development vitro, but highest concentration (100 ng/mL) reduced percentage normal when compared with tissues 10 ng/mL BMP-2. During culture...
Abstract This study aims to investigate the effects of melatonin on follicular growth, viability and ultrastructure, as well levels mRNA for antioxidant enzymes, reactive oxygen species (ROS) meiotic progression in oocytes from vitro cultured bovine early antral follicles. To this end, isolated follicles (500–600 μm) were TCM‐199 + alone or supplemented with 10 −6 , −7 −8 M at 38.5°C 5% CO 2 8 days. Follicle diameters evaluated days 0, 4 culture. At end culture, chromatin configuration,...
The aim of this study was to evaluate the effect follicular fluid collected from bovine dominant follicles (bFF) on in vitro development goat preantral and determine best time add supplement culture medium. were isolated randomly distributed into four treatments absence (control) or presence 10% bFF added Days 0 (FF0-18), 6 (FF6-18) 12 (FF12-18) onwards. After 18 days, assessed based survival, antral cavity formation, increased diameter as well fully grown oocyte (>110 μm) viability meiosis...
This study aimed to investigate the effects of different concentrations N-acetylcysteine (NAC) on growth, antrum formation, viability, and ultrastructure bovine secondary follicles cultured in vitro for 18 days. To this end, were TCM-199+ medium alone or supplemented with 1.0, 5.0, 25.0 mM NAC. Follicular viability (calcein-AM ethidium homodimer-1) evaluated at end culture period. The results showed that 1.0 NAC increased percentage growing fluorescence intensity calcein-AM when compared...
The objective this study was to determine the effect of phytohemagglutinin (PHA) on survival, growth and gene expression in caprine secondary follicles culturedin vitro. Secondary (∼0.2 mm) were isolated from cortex ovaries cultured individually for 6 days α-MEM+ supplemented with PHA (0, 1, 10, 50, 100, or 200 µg/mL). After culture, follicle diameter antrum formation, ultrastructure mRNA FSH receptors (FSH-R), proliferating cell nuclear antigen (PCNA), neuronal nitric oxide synthase...
Summary The role of activin-A in follicular development and on the mRNA expression levels different genes goat secondary follicles was evaluated. Goat (≥150 μm) were cultured for 18 days under control conditions or with addition either 50 100 ng/ml (Experiment 1). that code activin-A, ActR-IA, ActR-IB, ActR-IIA, ActR-IIB, follicle stimulating hormone receptor (FSH-R) P450 aromatase measured each condition 2). We observed after 6 culture, antrum formation rate higher cultures added than ( P...