A5053753112- Reproductive Biology and Fertility
- Reproductive Physiology in Livestock
- Reproductive System and Pregnancy
- Genetic and phenotypic traits in livestock
- Ovarian function and disorders
- Renin-Angiotensin System Studies
- Sperm and Testicular Function
- Animal Genetics and Reproduction
- Rural and Ethnic Education
- Ruminant Nutrition and Digestive Physiology
- Endometriosis Research and Treatment
- Business and Management Studies
- Effects of Environmental Stressors on Livestock
- Animal Behavior and Welfare Studies
- Pluripotent Stem Cells Research
- Coagulation, Bradykinin, Polyphosphates, and Angioedema
- Academic Research in Diverse Fields
- Growth Hormone and Insulin-like Growth Factors
- Assisted Reproductive Technology and Twin Pregnancy
- Trypanosoma species research and implications
- Fibroblast Growth Factor Research
- Microbial infections and disease research
- Apelin-related biomedical research
- Birth, Development, and Health
- Mycotoxins in Agriculture and Food
Universidade Federal de Santa Maria
2010-2024
Universidade Federal do Rio Grande do Sul
2017-2022
Universidade Federal de Pelotas
2017-2019
Instituto Federal Farroupilha
2019
Instituto Federal de Educação, Ciência e Tecnologia do Rio Grande do Sul
2018
The objective of this study was to characterize the profiles Ang-(1-7), MAS receptor, ACE 2 , NEP and PEP during ovulatory process in cattle. For study, 40 synchronized cows with follicular diameter ≥ 12 mm were ovariectomized at different time-points (0, 3, 6, 24 h) after i.m. application gonadotropin-releasing hormone (GnRH) induce a luteinizing surge. Follicular fluid collected for measuring Ang-(1-7) by radioimmunoassay. Theca granulosa cells isolated from preovulatory follicles evaluate...
It is generally understood that angiotensin II (AngII) promotes follicle atresia in rats, although recent data suggested this may not be true cattle. In study, we aimed to determine vivo whether AngII alters development cattle, using intrafollicular injection of or antagonist into the growing dominant second largest subordinate follicle. Injection saralasin, an antagonist, inhibited follicular growth, and inhibitory effect was overcome by systemic FSH supplementation. did affect whereas...
Oocyte meiotic resumption is triggered by the ovulatory gonadotropin surge; in cattle, angiotensin II (AngII) and prostaglandins (PG) are key mediators of this gonadotropin-induced event. Here, we tested hypothesis that progesterone (P(4)) also involved oocyte induced surge. In Experiment I, P(4) nuclear maturation a dose-dependent manner using coculture follicular hemisections cumulus-oocyte complexes. second experiment, an vivo model, injection mifepristone (MIFE; receptor antagonist) at...
The objective was to determine the effects of eCG given on day of, or 2 days before removal an intravaginal progestin device, ovarian follicle diameter, luteal volume, serum progesterone (P4) concentrations, and pregnancy per insemination in a fixed-time AI (FTAI) protocol. Lactating, anestrous, multiparous Bos taurus cross beef cows, 40 60 postpartum, were estradiol benzoate (2 mg im) device containing 250 medroxyprogesterone acetate Day 0 cloprostenol (0.265 mg) 6. Intravaginal devices...
Fibroblast growth factors (FGFs) are involved in paracrine control of follicle development. It was previously demonstrated that FGF10 decreases estradiol (E 2 ) secretion granulosa cell culture and theca mRNA expression is decreased healthy follicles from abattoir ovaries. The main objectives this study were to evaluate FGFR2b during follicular development vivo , the effect on using Bos taurus cows as a model, gain more insight into mechanisms through which inhibits steroidogenesis....
Angiotensin II (AngII) has a role in ovarian follicle development, ovulation, and oocyte meiotic resumption. The objective of the present study was to characterise AngII profile mRNA encoding RAS proteins bovine follicular wave. Cows were ovariectomised when size between largest (F1) second (F2) not statistically different (day 2), slightly 3), or markedly 4). measured fluid abundance genes angiotensin-converting enzyme (ACE), (pro)renin receptor, renin-binding protein (RnBP) evaluated cells...
The present study evaluated whether the gonadotrophin surge modulates components of renin–angiotensin system and angiotensin II (Ang II) plays a role in production hormones by follicular cells during ovulatory process. In Experiment 1, cows were ovariectomised at various times (0, 3, 6, 12 24h) after GnRH injection to obtain preovulatory follicles. concentration Ang fluid increased reached peak 24h, concomitant with angiotensinogen (AGT) mRNA expression granulosa cells. AGT was not expressed...
ABSTRACT: Fixed-time artificial insemination (FTAI) is a reproductive technology that aids in obtaining an appropriate time to perform single (AI), thus reducing the number of inseminations per sow bred. FTAI protocols can either be based on estrus detection or day weaning, aiming synchronize ovulation using inducers. The involving usually employ porcine luteinizing hormone (pLH) as inducer and, general, satisfactory performance observed. For weaning day, main used analog...
The use of oil to avoid water evaporation from cell culture has several disadvantages, amongst which there is the migration compounds media and media. aim this study was evaluate osmolality a system using four-well plates with in central hole as an alternative vitro bovine embryo production (IVP). In addition, changes oocyte washing medium were assessed 35mm dishes or without 2 mL silicon overlay. Osmolality changed great deal over time after 60 minutes on 39°C heated plate (291 mOsm kg-1),...
The main clinical feature associated with hyperandrogenism in polycystic ovary syndrome (PCOS) humans is hirsutism, where hair increases its length, pigmentation, and particularly diameter. Currently, it not known whether PCOS animal models also exhibit changes the hair. Therefore, aim of this study was to explore wool characteristics sheep prenatally androgenized (PA) testosterone propionate. After 4 13 months life, collected from top shoulder both females males (both controls). offspring...
The kallikrein-kinin system (KKS) has been described as an important mediator of physiologic processes. Kallikreins use kininogen (KNG) substrate to generate bradykinin, the main active peptide KKS that acts through two types receptors, B(1)R and B(2)R. goal this study was characterize some components in different compartments ovary during bovine ovulation process. KNG, B(2)R mRNA expression patterns were assessed theca granulosa cells, well bradykinin concentration kallikrein-like activity...
There are no efficient fixatives for histological analyses of ovarian tissue that permit an appropriate morphological analysis ovary and their cells. The correct processing the is essential to avoid misinterpretation between follicular degeneration due physiological process atresia with signs signals poor fixation. This study aimed identify best fixative better fixation period functional unit ovary: follicle. Ovaries (n = 5) from five adult cows were collected local abattoirs. Each was...
The aim of this study was to evaluate butaphosphan and cyanocobalamin effects on the production oocytes in vivo vitro embryo (IVP) during an ovum pick up (OPU) protocol. Thirty-six cows were homogeneously divided into two groups. group (CAT, n = 17), received 4500 mg 2.25 control (CONT, 19) placebo (NaCl 0.9%). Treatment performed at 14, 9, 5 d prior follicular aspiration (FA) sessions each cow subjected three OPU IVP. CAT showed high (P < 0.05) number aspirated follicles between 3 6 mm...
ABSTRACT: Gestation length in swine has a considerable amplitude and both early delayed parturition are common. This variation increases the occurrence of unassisted farrowing could lead to wide-ranging age at weaning for piglets born from one batch. Supervision sow is crucial reduce mortality neonate piglets. To facilitate assistance, induction using prostaglandin F2α (PGF) been widely used batch systems, whereby synchronization would concentrate time farrowing, allowing better organization...
Cytokines are proteins secreted by cells of innate and acquired immunity, produced in response to various antigens responsible for mediating several function these cells. Our study evaluated the profile cytokines interleukin 5 (IL-5) interferon gamma (IFN-γ), induced lymphocytes broiler chickens secondary fungal metabolites ochratoxin A (OTA) deoxynivalenol (DON) at concentrations 0.001, 0.01, 0.1 1 μg/ml. The quantification was analysed 24, 48 72 h after incubation with mycotoxins, using...
The significant role of C-type natriuretic peptide (CNP) and its receptor 2 (NPR2) in regulating oocyte meiotic maturation facilitating communication between oocytes surrounding cumulus cells has been well-documented various mammalian species, including mice, cattle swine. However, further investigation is needed to ascertain whether receptors (NPRs) are involved other essential ovarian functions. Hence, this study aimed explore the potential involvement NPRs regulation expansion bovine...
ABSTRACT This study aimed to evaluate the use of some testicular traits identify boars with low sperm morphological quality. The consistency (scores from 1 5), tone tonometry (mm), parenchyma echogenicity and heterogeneity ultrasound (pixel) were assessed in 402 mature (18.5 months on average). Sperm abnormality thresholds (≤ 25% total abnormalities, ≤ 5% heads, acrosome, neck or midpiece defects, 10% tail 15% cytoplasmic droplets) used classify as approved disapproved. Three classes formed...