A5052256988- Reproductive Biology and Fertility
- Reproductive Physiology in Livestock
- Genetic and phenotypic traits in livestock
- Pluripotent Stem Cells Research
- CRISPR and Genetic Engineering
- Sperm and Testicular Function
- Ovarian function and disorders
- Animal Disease Management and Epidemiology
- DNA Repair Mechanisms
- Effects of Environmental Stressors on Livestock
- Animal Genetics and Reproduction
- FOXO transcription factor regulation
- Rural development and sustainability
- Reproductive System and Pregnancy
- Renal and related cancers
- Reproductive Health and Technologies
- Estrogen and related hormone effects
- Diabetes, Cardiovascular Risks, and Lipoproteins
- Prenatal Screening and Diagnostics
- Bioenergy crop production and management
- Toxoplasma gondii Research Studies
- Cardiovascular Function and Risk Factors
- T-cell and Retrovirus Studies
- Radiopharmaceutical Chemistry and Applications
- Kruppel-like factors research
McGill University
2012-2023
Universidade Federal de Santa Maria
2008-2015
Ste. Anne's Hospital
2014
It is generally understood that angiotensin II (AngII) promotes follicle atresia in rats, although recent data suggested this may not be true cattle. In study, we aimed to determine vivo whether AngII alters development cattle, using intrafollicular injection of or antagonist into the growing dominant second largest subordinate follicle. Injection saralasin, an antagonist, inhibited follicular growth, and inhibitory effect was overcome by systemic FSH supplementation. did affect whereas...
Oocyte meiotic resumption is triggered by the ovulatory gonadotropin surge; in cattle, angiotensin II (AngII) and prostaglandins (PG) are key mediators of this gonadotropin-induced event. Here, we tested hypothesis that progesterone (P(4)) also involved oocyte induced surge. In Experiment I, P(4) nuclear maturation a dose-dependent manner using coculture follicular hemisections cumulus-oocyte complexes. second experiment, an vivo model, injection mifepristone (MIFE; receptor antagonist) at...
Introduction: This study was based on the hypothesis that some components of angiotensin-(1-7) (Ang-(1-7)) system are differentially expressed during follicular development and can be involved in health/atresia transition bovine. Material methods: The largest (F1) second follicles (F2) were collected from cows before (Day 2), 3), or after 4) expected moment deviation. In experiment, F1 induced to atresia through intrafollicular injection fulvestrant (estrogen receptor-antagonist) and, both...
Recent studies have shown that DNA damage affects embryo development and also somatic cell reprogramming into induced pluripotent stem (iPS) cells. It has been treatment with histone deacetylase inhibitors (HDACi) improves of embryos produced by nuclear transfer (SCNT) enhances reprogramming. There is evidence increasing acetylation at the sites double-strand breaks (DSBs) critical for repair. Therefore, we hypothesized HDACi programming facilitating To test this hypothesis, first...
Studies in different species, including human, mice, bovine, and swine, demonstrated that early-cleaving embryos have higher capacity to develop the blastocyst stage produce better quality with superior establish pregnancy than late-cleaving embryos. It has also been shown experimentally induced DNA damage delays embryo cleavage kinetics reduces formation. To gain additional insights into effects of genome on development, present study compared occurrence double-strand breaks (DSBs)...
Estradiol (E2) receptors mediate E2 effects on cell proliferation and apoptosis under normal pathological conditions. However, the mechanisms involved in signaling are not completely understood. The objectives this study were to evaluate expression of estrogen (ESRs) during follicular selection cattle, effect intrafollicular injection fulvestrant (an antagonist ESRs) development transcript abundance granulosa cells. Granulosa cells obtained from two largest follicles around deviation, after...
Early-cleaving embryos are known to have better capacity reach the blastocyst stage and produce quality compared late-cleaving embryos. To investigate significance of endoplasmic reticulum (ER) stress on early embryo cleavage kinetics development, porcine produced in vitro were separated into early- groups then cultured absence or presence ER inhibitor tauroursodeoxycholic acid (TUDCA). Developing collected at days 3 7 culture for assessment status, incidence DNA double-strand breaks (DSBs),...
Phosphorylated histone H2A.x (H2AX139ph) is a key factor for the repair of DNA double-strand breaks (DSBs) and presence H2AX139ph foci indicates sites DSBs. In this study, we characterized during in vitro development porcine embryos produced by IVF somatic cell nuclear transfer (SCNT). Pronuclear stage had, on average, 9.2 per pronucleus. The number was higher 2-cell-stage than 4-cell-stage fixed at 48 h post-fertilization. percentage H2AX139ph-positive nuclei SCNT that were activated with...
Oocytes collected from prepubertal animals are known to be less developmentally competent than those adult animals. There is evidence suggesting that acquisition of developmental competence in bovine oocytes may linked the expression profile genes granulosa cells (GCs). Cumulus–oocyte complexes (COC) and GCs were 12 Holstein heifers between 2 6 months age (nine follicle‐stimulating hormone [FSH] treated three untreated) eight FSH‐treated cows. The COCs matured, fertilized, cultured vitro...
DNA double-strand breaks (DSBs) are less frequent than single-strand but have more harmful consequences on cell survival and physiology. Homologous recombination (HR) nonhomologous end-joining (NHEJ) the two main pathways that responsible for DSB repair in eukaryotic cells, their importance preservation of genome stability totipotent blastomeres early developing embryos has not been determined. In this study, we observed chemical inhibition HR or both pathways, NHEJ alone, increased number...
The severe loss of body condition score (BCS) during early lactation period has been associated with infertility in cows. However, the mechanisms are not fully understood. aim this study was to examine effect BCS on liver health, and ovarian functions cows lactation. Retrospectively multiparous from two farms were categorized based units (1-5 scale) as Moderate (MOD, < 0.75 units; n = 11) or Severe (SEV, ≥ 9) groups. From Week -3 7, relative calving, MOD SEV lost average 0.4 1.0-unit BCS,...
The aim was to develop a timed artificial insemination (TAI) system in suckled beef cows. Cows (n=227), 60-80 days postpartum, received estradiol benzoate (5mg) and vaginal device containing 250µg of medroxyprogesterone acetate (MPA; day 0). On six, cloprostenol (125µg) eCG (400IU) were administrated calves weaned for 88h. devices removed on seven (BioRep group) or eight (TAI group). All cows TAI group BioRep that did not exhibit standing estrus GnRH (100µg) 9. In experiment I, the...
Contents The growth factor receptor‐bound protein 14 (Grb14) is a cellular adapter belonging to the Grb7 family of proteins. Studies with human and rodent cells have demonstrated that Grb14 acts as negative regulator tyrosine kinase receptor signalling through MAPK PI3K pathways. In cattle, receptors are activated during follicular development but role in this process has not yet been investigated. Therefore, aim present study was characterize mRNA expression ovarian somatic deviation...
Cell permeabilization followed by exposure to cytoplasmic extracts of oocytes has been proposed as an alternative transduction transcription factors for inducing pluripotency in cultured somatic cells. The main goal this study was investigate the effect treating porcine fibroblast cells with GV-stage oocyte (OEx) inhibition histone deacetylases Scriptaid (Scrip) on formation stem cell-like colonies and expression genes encoding chromatin-modifying enzymes. Stem start developing ∼2 weeks...
Longevity is a key component of sustainable dairy farming. High-yielding cows often suffer from ovarian dysfunction and infertility leading to reduced reproductive productive longevity. Infertility has been attributed the metabolic stress during transition period. However, potential alterations that may dysregulate functions have not completely cataloged. Our objective was characterize parameters In first experiment, we examined profiles in circulation pinpoint time-points major changes. We...
CRISPR/Cas9 technology is a powerful tool used for genome manipulation in different cell types and species. However, as with all new technologies, it still requires improvements. Different factors can affect CRISPR/Cas efficiency zygotes, which influence the total cost complexity creating large-animal models research. This study evaluated importance of zygote cycle stage between early-injection (within 6 h post activation/fertilization) versus late-injection (14-16 when components were...
Summary The Grb14 protein is a member of the Grb7 family. This family acts by binding to tyrosine kinase receptors, promoting cell proliferation and differentiation. There evidence involvement factors in bovine oocyte maturation process. However, has not been studied for cumulus–oocyte complexes (COCs). aim present study was characterize mRNA expression COCs during follicular development. Furthermore, we demonstrated that regulated estradiol. assessed 480 from follicles different sizes (1–3,...
Oocyte competence and reproductive biology in prepubertal heifer calves are not fully understood. Multiple publications have reported high oocyte yields recovered from aged 2–6 months old but low embryo development rates following vitro production. The objective of this study was to characterise the developmental oocytes young calves. We report herein oocyte/embryo obtained 6 Holstein that were subjected gonadotropin stimulation laparoscopic ovum pick-up (LOPU) every 2 weeks, starting at age...
The recovery of an exceptionally high number cumulus–oocyte complexes (COC) in a single ovum pickup (OPU) session is rare, and thus seldom reported the literature. In present report, we describe one these cases. data refers to OPU performed by author A. O. Resende commercial beef farm, located rural area city Barretos, Brazil, on October 15, 2019. Breed heifers (N=12) underwent at random stage oestrous cycle without any hormonal treatment. Follicle aspirations were following standard...