A5038235319- Reproductive Physiology in Livestock
- Reproductive Biology and Fertility
- Genetic and phenotypic traits in livestock
- Rural development and sustainability
- Ovarian function and disorders
- Reproductive System and Pregnancy
- Kruppel-like factors research
- Endometriosis Research and Treatment
- Neuroendocrine regulation and behavior
- FOXO transcription factor regulation
- Uterine Myomas and Treatments
- Growth Hormone and Insulin-like Growth Factors
- Infant Nutrition and Health
- Neonatal Respiratory Health Research
- Genetic Mapping and Diversity in Plants and Animals
McGill University
2016-2025
ABSTRACT The endocannabinoid receptors Cnr1 and Cnr2 have been found in reproductive organs such as the oviduct uterus. These bind to endocannabinoids, arachinodoylethanolamine (AEA) arachinodoylglycerol (2‐AG), respectively. Both cannbinoid investigated for their role implantation fertilization. However, not much is explored terms of ovarian granulosa cells. As these two (especially Cnr1) affinity towards major component Cannabis, tetrahydrocannabinol (THC), its usage raises concerns about...
Ovulation is triggered by gonadotropin surge-induced signaling cascades. To study the role of extracellular signal-regulated kinase 1/2 (ERK1/2) in bovine ovulation, we administered pharmacological inhibitor, PD0325901, into preovulatory dominant follicle intrafollicular injection. Four five cows treated with 50 µM PD0325901 failed to ovulate. uncover molecular basis anovulation ERK1/2-inhibited cows, collected granulosa and theca cells from Vehicle follicles. Next-generation sequencing cell...
Oocytes collected from prepubertal animals are known to be less developmentally competent than those adult animals. There is evidence suggesting that acquisition of developmental competence in bovine oocytes may linked the expression profile genes granulosa cells (GCs). Cumulus–oocyte complexes (COC) and GCs were 12 Holstein heifers between 2 6 months age (nine follicle‐stimulating hormone [FSH] treated three untreated) eight FSH‐treated cows. The COCs matured, fertilized, cultured vitro...
Follicle-stimulating hormone (FSH) regulates ovarian follicular development through a specific gene expression program. We analyzed FSH-regulated transcriptome and histone modification in granulosa cells during development. used super-stimulated immature mice collected before 48 h after stimulation with equine chorionic gonadotropin (eCG). profiled the using RNA-sequencing (N = 3/time-point) genome-wide trimethylation of lysine 4 H3 (H3K4me3; an active transcription marker) chromatin...
The severe loss of body condition score (BCS) during early lactation period has been associated with infertility in cows. However, the mechanisms are not fully understood. aim this study was to examine effect BCS on liver health, and ovarian functions cows lactation. Retrospectively multiparous from two farms were categorized based units (1-5 scale) as Moderate (MOD, < 0.75 units; n = 11) or Severe (SEV, ≥ 9) groups. From Week -3 7, relative calving, MOD SEV lost average 0.4 1.0-unit BCS,...
Abolition of the LH-induced ERK1/2 pathway leads to dramatic changes in gene expression granulosa cells, subsequently abrogating ovulation. Here we explored whether sustained signaling beyond immediate-early hours LH surge is important for ovulation mice. First, examined effect inhibition activity at 4 h after hCG stimulation on superovulated immature Treatment with inhibitor PD0325901 post-hCG disrupted follicular rupture without altering cumulus expansion, oocyte meiotic maturation and...
Abstract Intestinal nematode infections common during pregnancy have recently been shown to impacts that extend their uninfected offspring including altered brain gene expression. If maternal immune signals reach the neonatal brain, they might alter neuroimmune development. We explored expression of genes associated with four distinct types T cells (Th1, Th2, Th17, Treg) and leukocyte transendothelial migration endocytosis transport across blood–brain barrier (BBB) in postnatal...
Longevity is a key component of sustainable dairy farming. High-yielding cows often suffer from ovarian dysfunction and infertility leading to reduced reproductive productive longevity. Infertility has been attributed the metabolic stress during transition period. However, potential alterations that may dysregulate functions have not completely cataloged. Our objective was characterize parameters In first experiment, we examined profiles in circulation pinpoint time-points major changes. We...