A5012756279- Cellular transport and secretion
- Cell Image Analysis Techniques
- Terahertz technology and applications
- Advanced Fluorescence Microscopy Techniques
- Superconducting and THz Device Technology
- Radio Frequency Integrated Circuit Design
- Advanced biosensing and bioanalysis techniques
- Advanced Biosensing Techniques and Applications
- Cell Adhesion Molecules Research
- Cellular Mechanics and Interactions
- Semiconductor Quantum Structures and Devices
- Advancements in PLL and VCO Technologies
- Extracellular vesicles in disease
- RNA regulation and disease
- Photonic and Optical Devices
- Microwave Engineering and Waveguides
- Microfluidic and Bio-sensing Technologies
- MicroRNA in disease regulation
- Optical Coherence Tomography Applications
- Endoplasmic Reticulum Stress and Disease
- Single-cell and spatial transcriptomics
Heidelberg University
2010-2019
This work presents, to our knowledge, the first completely passive imaging with human-body-emitted radiation in lower THz frequency range using a broadband uncooled detector. The sensor consists of Si CMOS field-effect transistor an integrated log-spiral antenna. was measured exhibit rather flat responsivity over 0.1–1.5-THz range, values optical and noise-equivalent power around 40 mA/W 42 pW/ Hz , respectively. These are good agreement simulations which suggest even broader exceeding 2.0...
miRNA cluster miR-17-92 is known as oncomir-1 due to its potent oncogenic function. a polycistronic that encodes 6 miRNAs, and can both facilitate inhibit cell proliferation. Known targets of miRNAs encoded by this are largely regulators cycle progression apoptosis. Here, we show sharing the seed sequence miR-17 exert their influence on one most essential cellular processes – endocytic trafficking. By mRNA expression analysis identified regulation trafficking potentially be achieved...
The spread of practical terahertz (THz) systems dedicated to the telecommunication, pharmacy, civil security, or medical markets requires use mainstream semiconductor technologies, such as complementary metal-oxide-semiconductor (CMOS) lines. In this paper, we discuss operation a CMOS-based free space all-electronic system operating near 250 GHz, exhibiting signal-to-noise ratio (SNR) with 62 dB in direct detection regime for one Hz equivalent noise bandwidth. It combines state-of-the-art...
α2β1 integrin is one of the most important collagen-binding receptors and has been implicated in numerous widely spread thrombotic immune diseases. a potent tumour suppressor its downregulation associated with increased metastasis poor prognosis breast cancer. Currently, very little known about mechanism regulating cell surface expression trafficking. Here, using quantitative fluorescent microscopy-based RNAi assay, we investigated impact 386 cytoskeleton-associated or regulatory genes on...
High-throughput microscopy is an effective tool for rapidly collecting data on a large scale. However, high throughput comes at the cost of low spatial resolution. Here we introduce correlative light by combining fast automated widefield imaging, confocal and super-resolution microscopy. We demonstrate potential this approach scalable experiments. The workflow consists robust selecting cells interest wide-field screening microscope resolution subsequently re-localizing those with micrometer...
We applied fluorescence microscopy-based quantitative assays to living cells identify regulators of endoplasmic reticulum (ER)-to-Golgi trafficking and/or Golgi complex maintenance. first validated an automated procedure factors which influence Golgi-to-ER relocalization GalT-CFP (β1,4-galactosyltransferase I-cyan fluorescent protein) after brefeldin A (BFA) addition wash-out. then tested 14 proteins that localize the ER when overexpressed for a role in ER-to-Golgi trafficking. Nine them...
This paper presents a fully-electronic wireless link operating at the 250 GHz frequency. The key elements of developed system are voltage-controlled harmonic oscillator implemented in 65 nm complementary metal-oxide-semiconductor technology (CMOS) and quasi-optical detector with resonantantenna- coupled field-effect transistor completed 90 CMOS. source is optimized for third emission 252 radiated power reaching up to –11 dBm (decibels reference one milliwatt) level. has resonance maximum 254...
Due to high associated costs and considerable time investments of cell-based screening, there is a strong demand for new technologies that enable preclinical development tests diverse biologicals in cost-saving time-efficient manner. For those reasons we developed the high-density cell array (HD-CA) platform, which miniaturizes screening form preprinted ready-to-run arrays. With HD-CA technology, up 24,576 samples can be tested single experiment, thereby saving microscopy-based by 75%....
This work presents to our knowledge the first completely passive imaging of human body radiation below 1 THz at room temperature without additional illumination or cooling. The successful image shows an impressive sensitivity limit a terahertz FET detector (TeraFET). We made 2.7 cm × 4 size hand with spatial resolution mm and scanning speed equal <sup xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">2</sup> /100 s. SNR is only 2.5 dB NETD 4.8 K.