A5030289019- Neuroendocrine regulation and behavior
- Neuroscience of respiration and sleep
- Stress Responses and Cortisol
- Neuropeptides and Animal Physiology
- Regulation of Appetite and Obesity
- Infant Health and Development
- Circadian rhythm and melatonin
- Biochemical Analysis and Sensing Techniques
- Sleep and Wakefulness Research
- Anesthesia and Neurotoxicity Research
- Pain Mechanisms and Treatments
- Biochemical effects in animals
- Musculoskeletal pain and rehabilitation
- Ion Channels and Receptors
- Hypothalamic control of reproductive hormones
- Orthopedic Infections and Treatments
- Surgical site infection prevention
- Spaceflight effects on biology
- Pain Management and Placebo Effect
- Total Knee Arthroplasty Outcomes
- Cardiovascular, Neuropeptides, and Oxidative Stress Research
- Peripheral Nerve Disorders
- Spine and Intervertebral Disc Pathology
- Pediatric Pain Management Techniques
- Vagus Nerve Stimulation Research
University of Occupational and Environmental Health Japan
2015-2024
Saiseikai Fukuoka General Hospital
2012
Abstract Oxytocin is involved in pain transmission, although the detailed mechanism not fully understood. Here, we generate a transgenic rat line that expresses human muscarinic acetylcholine receptors (hM3Dq) and mCherry oxytocin neurons. We report clozapine-N-oxide (CNO) treatment of our oxytocin-hM3Dq-mCherry rats exclusively activates neurons within supraoptic paraventricular nuclei, leading to activation locus coeruleus (LC) dorsal raphe nucleus (DR), differential gene expression...
Abstract Various studies contributed to discover novel mechanisms of central arginine vasopressin (AVP) system responsible for the behaviour albeit endogenous activation. We established a transgenic rat line which expresses both human muscarinic acetylcholine receptors (hM3Dq), ligand is clozapine-N-oxide (CNO), and mCherry fluorescence specifically in AVP neurons. The neurons that indicate expression hM3Dq gene were observed suprachiasmatic (SCN), supraoptic (SON), paraventricular nuclei...
Abstract Background Antimicrobial agents are administered via intramedullary antibiotic perfusion (iMAP)/intrasoft tissue (iSAP) to infected lesions control osteoarticular and soft infections. Continuous local (CLAP) has been reported be useful. This study aimed investigate the outcomes of DAIR combined with CLAP for chronic PJI after total knee arthroplasty performed at our hospital. Subjects methods Six patients (male; one case, female; five cases, mean age 79.5 years (70–94)) underwent...
Oxytocin (OXT) is a well-known neurohypophysial hormone that synthesised in the paraventricular (PVN) and supraoptic nuclei (SON) of hypothalamus. The projection magnocellular neurosecretory cells, which synthesise OXT arginine vasopressin PVN SON, to posterior pituitary plays an essential role mammalian labour lactation through its peripheral action. However, previous studies have shown parvocellular OXTergic cells PVN, project medulla spinal cord, are involved various physiological...
Oxytocin (OXT)-containing neurosecretory cells in the parvocellular divisions of paraventricular nucleus (PVN), which project to medulla and spinal cord, are involved various physiological functions, such as sensory modulation autonomic processes. In present study, we examined OXT expression hypothalamo-spinal pathway, well hypothalamo-neurohypophysial system, includes magnocellular PVN supraoptic (SON), after s.c. injection saline or formalin into hindpaws transgenic rats that express...
Peripheral anorectic hormones, such as glucagon-like peptide (GLP)-1, cholecystokinin (CCK)-8 and leptin, suppress food intake. The newly-identified neuropeptide, nesfatin-1, is synthesised in both peripheral tissues the central nervous system, particularly by various nuclei hypothalamus brainstem. In present study, we examined effects of i.p. administration GLP-1 CCK-8 co-administrations leptin at subthreshold doses confirmed measurement intake, on nesfatin-1-immunoreactive (-IR) neurones...
Various types of acute/chronic nociceptive stimuli cause neuroendocrine responses such as activation the hypothalamo-neurohypophysial (oxytocin [OXT] and arginine vasopressin [AVP]) system hypothalamo-pituitary adrenal (HPA) axis. Chronic multiple-arthritis activates OXT/AVP system, but effects acute mono-arthritis on in same animals has not been simultaneously evaluated. Further, AVP, corticotropin-releasing hormone (CRH), predominantly HPA axis chronic multiple-arthritis, participation AVP...
Abstract Orexin-A and -B, ghrelin are potent orexigenic peptides. The effects of ACT462206, a novel dual orexin receptor antagonist (DORA), on ghrelin-induced feeding were examined in adult male Wistar rats. Hyperphagia induced by the intracerebroventricular (icv) administration was significantly suppressed for at least 2 h pretreatment with icv DORA. A marked increase observed number neurons showing Fos immunoreactivity paraventricular nucleus, arcuate nucleus lateral hypothalamic area...
Calcium balance is important in bone homeostasis. The transient receptor potential vanilloid (TRPV) channel a nonselective cation permeable to calcium and activated by various physiological pharmacological stimuli. TRPV1 TRPV4, particular, have roles intracellular Ca2+ signaling extracellular homeostasis cells. TRPV4 separately mediate osteoclast osteoblast differentiation, deficiency any of these channels leads increased mass. However, it remains unknown whether mass increases the absence...
During cancer chemotherapy, drugs such as 5-HT3 receptor antagonists have typically been used to control vomiting and anorexia. We examined the effects of oxytocin (OXT), which has linked appetite, on cisplatin-induced anorexia in rats. Fos-like immunoreactivity (Fos-LI) expressed supraoptic nucleus (SON), paraventricular (PVN), area postrema solitary tract (NTS) after intraperitoneal (ip) administration cisplatin. also fluorescence intensity OXT-mRFP1 ip cisplatin transgenic The mRFP1 was...
Osteoarthritis (OA) causes chronic joint pain and significantly impacts daily activities. Hence, developing novel treatment options for OA has become an increasingly important area of research. Recently, studies have reported that exogenous, as well endogenous, hypothalamic-neurohypophysial hormones, oxytocin (OXT) arginine-vasopressin (AVP), contribute to nociception modulation. Moreover, the parvocellular OXT neurone (parvOXT) extends its projection superficial spinal dorsal horn, where it...
Acute loss of kidney function is a critical internal stressor. Arginine vasopressin (AVP) present in the parvocellular division paraventricular nucleus (PVN) plays key role regulation stress responses. However, hypothalamic AVP dynamics during acute dysfunction remain unclear. In this study, we investigated effects bilateral nephrectomy on AVP, using transgenic rat line that expressed AVP-enhanced green fluorescent protein (eGFP). The eGFP intensities PVN were dramatically increased after...
Abstract Cisplatin is one of the most potent anti-cancer drugs, though several side effects can induce stress responses such as activation hypothalamic–pituitary adrenal (HPA) axis. Arginine vasopressin (AVP) and corticotrophin-releasing hormone (CRH) expressed in parvocellular division paraventricular nucleus (pPVN) play an important role stress-induced HPA We aimed to evaluate whether intraperitoneal (i.p.) administration cisplatin could activate neurons pPVN, using a transgenic rat model...
Furosemide, which is used worldwide as a diuretic agent, inhibits sodium reabsorption in Henle's loop, resulting diuresis and natriuresis. Arginine vasopressin ( AVP ) synthesised the supraoptic nucleus SON ), paraventricular PVN suprachiasmatic of hypothalamus. The synthesis magnocellular neurones physiologically regulated by plasma osmolality blood volume contributes to water homeostasis increasing collecting duct. Central dynamics after peripheral administration furosemide remain unclear....