A5032047731- Osteoarthritis Treatment and Mechanisms
- 3D Printing in Biomedical Research
- Cellular Mechanics and Interactions
- Fuel Cells and Related Materials
- Pancreatic function and diabetes
- Mesenchymal stem cell research
- Electrospun Nanofibers in Biomedical Applications
- Electrocatalysts for Energy Conversion
- Liver physiology and pathology
- Wound Healing and Treatments
- Cell Adhesion Molecules Research
- Knee injuries and reconstruction techniques
- Cancer Cells and Metastasis
- Nanoparticle-Based Drug Delivery
- Periodontal Regeneration and Treatments
- Pluripotent Stem Cells Research
- Tendon Structure and Treatment
- Tissue Engineering and Regenerative Medicine
- Collagen: Extraction and Characterization
- Silk-based biomaterials and applications
- Ultrasound Imaging and Elastography
- Microfluidic and Capillary Electrophoresis Applications
- Advancements in Solid Oxide Fuel Cells
- Mathematical Biology Tumor Growth
- RNA Interference and Gene Delivery
Case Western Reserve University
2016-2025
Harvard University
2003
Shriners Hospitals for Children - Erie
2001-2002
Harvard University Press
2001
Massachusetts General Hospital
2000
Pennsylvania State University
1996-1998
In the recent past, remarkable advances in nanotechnology have generated nanoparticles of different shapes and sizes, which been shown to exhibit unique properties suitable for biomedical applications such as cancer therapy imaging. Obviously, all are not made equal. This becomes evident when we consider their transport behavior under blood flow microcirculation. this work, evaluated effect critical physical characteristics particle shape, size density on a nanoparticle's tendency marginate...
The goal of this study was to investigate the viability and synthetic function rat hepatocytes cocultured with 3T3-J2 fibroblasts in a small-scale microchannel flat-plate bioreactor without an internal membrane oxygenator under flow. Bioreactor channel heights ranged between 85 500 microm medium flow rates 0.06 4.18 mL/min. results showed that resulted significantly decreased hepatocytes, whereas were able maintain their function. shear stress calculations that, at lower wall stresses (0.01...
While the enhanced permeability and retention effect may promote preferential accumulation of nanoparticles into well-vascularized primary tumors, it is ineffective in case metastases hidden within a large population normal cells. Due to their small size, high dispersion organs, low vascularization, metastatic tumors are less accessible targeted nanoparticles. To tackle these challenges, we designed nanoparticle for vascular targeting based on an αvβ3 integrin-targeted nanochain particle...
Non-muscle myosin II (NMII) is reported to play multiple roles during cell migration and invasion. However, the exact biophysical of different NMII isoforms these processes remain poorly understood. We analyzed contributions NMIIA NMIIB in three-dimensional (3D) generating forces required for efficient invasion by mammary gland carcinoma cells. Using traction force microscopy microfluidic devices, we demonstrated that critical active protrusion, plays a major role applying on nucleus...
There is a strong need for tissue engineering scaffolds that are mechanically robust, exhibit good biocompatibility, and can be made from readily available materials. To this end, blends of commercially poly(ethylene glycol) diacrylate (PEGDA) with molecular weights 400 3400 were UV-crosslinked at total polymer concentrations varied systematically 20 to 40 wt %. The compressive strength cell viability determined each PEGDA mixture. modulus the was maximized when weight percent ratio...
These studies address critical technical issues involved in creating human mesenchymal stem cell (hMSC)/ scaffold implants for cartilage repair. include obtaining a high density and uniform spatial distribution within the scaffold, factors that are initiation homogeneity of chondrogenic differentiation. For any given initial seeding influences density, retention, which eventually will affect function construct. Here, we discuss development vacuum-aided technique HYAFF -11 sponges compared to...
Collagen-chondroitin sulfate biomaterial scaffolds have been used in a number of tissue-engineered products under development or the clinics. In this article, we describe new approach based on centrifugation for obtaining highly concentrated yet porous collagen scaffolds. Water uptake, chondroitin retention, morphology, mechanical properties, and tissue-engineering potential were investigated. Our results show that can lead to containing four times as much conventional unconcentrated...
Abstract Cellular therapies based on human pluripotent stem cells (hPSCs) offer considerable promise for treating numerous diseases including diabetes and end stage liver failure. Stem cell spheroids may be cultured in stirred bioreactors to scale up production numbers relevant use humans. Despite significant progress bioreactor culture of cells, areas improvement remain. In this study, we demonstrate that microfluidic encapsulation hPSCs formation spheroids. A co-axial droplet device was...
Abstract This study investigates the photothermal properties of citrate‐capped gold nanoparticles (Au NPs) dispersed in agarose gel, examining various sizes and concentrations, particularly within a low‐concentration range (0.2–2.5 nM). Heat transfer measurements are conducted on Au NP hydrogels using laser‐light induced heating, revealing size‐ concentration‐dependent temperature increase compared to plain gel matrix. Experimental data, combined with finite‐element analysis, demonstrate...
The ability to maintain functional hepatocytes has important implications for bioartificial liver development, cell-based therapies, drug screening, and tissue engineering. Several approaches can be used restore hepatocyte function in vitro, including coating a culture substrate with extracellular matrix (ECM), encapsulating cells within biomimetic gels (Collagen- or Matrigel-based), co-cultivation other cells. This paper describes the use of bioactive heparin-based core-shell microcapsules...
Abstract Experimental investigation of the photothermal properties carboxylated nanodiamond (ND) particles in agarose gel base medium over a range very low particle concentrations from 6.55 × 10 −5 to 2.29 −2 % v/v and two different sizes (35 160 nm) is carried out. Laser‐induced spot heating gels with trace amounts ND lead large increase temperatures away compared gels. These increases are inconsistent any thermal conductivity associated incorporation particles. UV‐visible Raman...
When cationic impurities, such as Na+, Ca2+, ammonium or metal cations, are present in a polymer electrolyte membrane fuel cell (PEMFC) the performance of PEMFC can be significantly reduced. A half model was developed to explain most relevant processes causing degradation cells under contamination. In contaminated with foreign cations exchange current density and limiting decreases. These effects explained by accounting for concentration available protons CCL transport diffusion migration.
Several configurations of extracorporeal bioartificial liver devices have been developed for the potential treatment fulminant hepatic failure or as a bridge to transplantation. Recently, we microchannel flat-plate bioreactor with an internal membrane oxygenator in which porcine hepatocytes are cultured monolayer on bottom glass surface. In present study, investigated synthetic function both vitro and vivo flow circuit models. vitro, albumin synthesis was stable up 4 days perfusion. vivo,...
Tissue engineering is a possible method for long-term repair of cartilage lesions, but current tissue-engineered constructs have inferior mechanical properties compared to native cartilage. This problem may be due the lack an oriented structure in at microscale that present tissue. In this study, we utilize contact guidance develop with architecture improved chondrogenesis and function. Stable channels varying dimensions were formed collagen-based polydimethylsiloxane membranes via...