A5072902664- Genomics, phytochemicals, and oxidative stress
- Redox biology and oxidative stress
- Liver physiology and pathology
- Inflammatory mediators and NSAID effects
- Cell death mechanisms and regulation
- Glutathione Transferases and Polymorphisms
- Cancer, Hypoxia, and Metabolism
- Cancer Mechanisms and Therapy
- Mesenchymal stem cell research
- FOXO transcription factor regulation
- Organ Transplantation Techniques and Outcomes
- Peroxisome Proliferator-Activated Receptors
- Phagocytosis and Immune Regulation
- Virus-based gene therapy research
- Wound Healing and Treatments
- Angiogenesis and VEGF in Cancer
- Cancer, Lipids, and Metabolism
- Heat shock proteins research
- Flavonoids in Medical Research
- Calpain Protease Function and Regulation
- Antimicrobial Peptides and Activities
- S100 Proteins and Annexins
- Sirtuins and Resveratrol in Medicine
- Nitric Oxide and Endothelin Effects
- Mechanisms of cancer metastasis
Gachon University
2012-2023
Charles R. Drew University of Medicine and Science
2007-2013
NovaCell Technology (South Korea)
2007-2010
Celltrion (South Korea)
2010
Korea National Institute of Health
2000-2004
Pediatrics and Genetics
2003
Johns Hopkins University
1996-1997
Johns Hopkins Medicine
1997
Wake Forest University
1994-1996
Atrium Health Wake Forest Baptist
1994-1996
Mesenchymal stem cells (MSCs) are multipotent progenitor with the ability to secrete growth factors. Because wound healing is associated fibroblast and extracellular matrix (ECM) in dermis epidermis, we used resolve question of whether or not MSCs regulate vitro via a regenerative function. Using cell proliferation assay, demonstrated that conditioned media (CM) obtained from significantly enhanced survival cells. Moreover, by measurement mRNA protein, observed CM also promoted production...
We implemented a protein microarray approach to identify proteins that induce humoral response in colon cancer. Solubilized from the LoVo adenocarcinoma cell line were separated into 1760 fractions, arrayed onto nitrocellulose-coated slides, and hybridized with individual sera 15 newly diagnosed patients cancer, lung healthy subjects. 39/1760 fractions showed enhanced reactivity cancer (p < 0.01) relative controls. A distinct pattern of was observed One fraction exhibited 9/15 subjected mass...
We have shown previously that the multifunctional cytokine scatter factor/hepatocyte growth factor (SF/HGF) is elevated in human malignant gliomas. In this study we investigated how SF/HGF expression affects malignancy of U373 glioblastoma cell linein vivoandin vitro.Human gene transfer increased tumorigenicity by ≥20-fold and enhanced rate intracerebral xenografts 3- to 8-fold. had no effect on proliferation monolayers but their anchorage-independent colony formation soft agar 5- These...
Tomentosin is a natural sesquiterpene lactone extracted from various plants and widely used as medicine because it exhibits essential therapeutic properties. In this study, we investigated the anti-carcinogenic effects of tomentosin in human osteosarcoma MG-63 cells by performing cell migration/viability/proliferation, apoptosis, reactive oxygen species (ROS) analysis assays. were treated with doses tomentosin. After treatment tomentosin, analyzed using MTT assay, colony formation counting...
We investigated the anti-cancer effects of ESC in human colon cancer LoVo cells. Cell counting assay results showed that inhibited proliferation cycle arrest cell was arrested during G0/G1 phase ESC-treated Western blot inhibitory proteins p53, p27, and p21 were increased, cyclin D1, progressive protein, decreased. Sp1 is a transcription factor regulating proliferation, decreased Annexin V/propidium iodide staining induces apoptosis Bax, cleaved caspase -3, -7, -9, poly(ADP-ribose)...
This study was focused on investigating the anticancer potential of indole-3-carbinol (I3C) against lung cancer H1299 cells via an increase in ROS levels. To investigate induction growth arrest and/or cell death cells, a cycle assay, terminal deoxynucleotidyl transferase (TdT)-mediated dUTP-biotin nick-end labeling (TUNEL) and reactive oxygen species (ROS) detection assay were performed. Through TUNEL we detected I3C-induced DNA fragmentation. Fluorescence-activated sorting (FACS) analysis...
Natural flavonoids are associated with anti-proliferation of cancer growth. However, the antioxidant and effects AE (aloe-emodin) have not been well studied. We investigated how affects proliferation hepatic hepatocellular carcinoma cells exerts an anti-cancer effect. The cytotoxic effect was demonstrated using MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide] assay Huh-7 were inhibited by treatment in both dose- time-dependent manners. IC(50) level ∼75 μM. also has...
Abstract Objectives We were interested in determining whether epidermal growth factor gene‐transfected mesenchymal stem cells ( EGF ‐ MSC ) would accelerate fibroblast migration and proliferation. Materials methods Fibroblasts cultured serum‐free conditioned media from ; RT PCR was performed to detect expression of gene s. protein levels cell culture supernatants assayed by ELISA proliferation ‐treated fibroblasts using MTT assay. Effects on evaluated scratch wound transmigration assays....
Acetylshikonin, a naphthoquinone, is pigment compound derived from Arnebia sp., which known for its anti‐inflammatory potential. However, anticarcinogenic effect has not been well investigated. Thus, in this study, we focused on investigating apoptotic effects against HCT‐15 and LoVo cells, are human colorectal cancer cells. MTT assay, cell counting colony formation assay have shown acetylshikonin treatment induced cytotoxic antiproliferative cells dose‐ time‐dependent manner. DNA...
This study was focused on investigating the anticancer potential of indole-3-carbinol (I3C) against osteosarcoma MG-63 and U2OS cells. A wound healing assay indicated that IC3 inhibited migration MTT, WST-1, colony formation assays revealed treatment cells with I3C decreased cell viability. Fluorescence-activated sorting (FACS) analysis showed induced apoptosis in a dose- time-dependent manner Moreover, via terminal deoxynucleotidyl transferase- (TdT-) mediated dUTP-biotin nick-end labeling...
There is substantial interest in the identification of circulating human tumor-derived proteins serum for purposes early cancer diagnosis. We have implemented an approach based on analysis microarray data tumor that may utility as biomarkers colon cancer. Expression obtained from a variety 283 tumors and normal tissues revealed defensin α6 was maximally expressed These findings were corroborated by reverse transcription-PCR, which cell lines LoVo, Caco2, HCT-15, SW480, SW620 showed...
Abstract The early diagnosis of colorectal cancer and the detection recurrence are central to effective treatment, as prognosis is directly related stage disease. When diagnosed at an early, localized stage, 5-year survival 90%. There substantial interest in identification circulating human tumor-derived proteins serum for purposes diagnosis. We have implemented approach based on analysis microarray data tumor that may utility biomarkers colon cancer. Expression obtained from a variety 290...