A5033079939- Advanced Fluorescence Microscopy Techniques
- Cell Image Analysis Techniques
- Diamond and Carbon-based Materials Research
- Advanced Electron Microscopy Techniques and Applications
- Advanced machining processes and optimization
- Advanced Surface Polishing Techniques
- Force Microscopy Techniques and Applications
- Gene expression and cancer classification
- Microplastics and Plastic Pollution
- Nanoparticles: synthesis and applications
- Nanoparticle-Based Drug Delivery
- Hydraulic and Pneumatic Systems
- Tunneling and Rock Mechanics
- Advanced ceramic materials synthesis
- Metal Alloys Wear and Properties
- Analytical Chemistry and Sensors
- Near-Field Optical Microscopy
- Additive Manufacturing and 3D Printing Technologies
- Integrated Circuits and Semiconductor Failure Analysis
- Advanced X-ray Imaging Techniques
- Laser Material Processing Techniques
- High-Temperature Coating Behaviors
- Characterization and Applications of Magnetic Nanoparticles
- Engineering Technology and Methodologies
- Tribology and Wear Analysis
Heidelberg University
2021-2024
Goethe University Frankfurt
2021-2024
University of Leeds
2019-2020
Keele University
2018-2020
University of Florida
2018
Indian Institute of Technology Madras
1991-2005
Nanodomains are intracellular foci which transduce signals between major cellular compartments. One of the most ubiquitous signal transducers, ryanodine receptor (RyR) calcium channel, is tightly clustered within these nanodomains. Super-resolution microscopy has previously been used to visualize RyR clusters near cell surface. A majority nanodomains located deeper cells have remained unresolved due limited imaging depths and axial resolution modalities. series enhancements made expansion...
Abstract DNA points accumulation for imaging in nanoscale topography (DNA-PAINT) is a super-resolution technique with relatively easy-to-implement multi-target imaging. However, image acquisition slow as sufficient statistical data has to be generated from spatio-temporally isolated single emitters. Here, we train the neural network (NN) DeepSTORM predict fluorophore positions high emitter density DNA-PAINT data. This achieves one minute. We demonstrate multi-colour of structure-conserved...
Naturally occurring paramagnetic species (PS), such as free radicals and metalloproteins, play an essential role in a multitude of critical physiological processes including metabolism, cell signaling, immune response. These highly dynamic can also act intrinsic biomarkers for variety disease states, while synthetic probes targeted to specific sites on biomolecules enable the study functional information tissue oxygenation redox status living systems. The work presented herein describes new...
Single-molecule localization microscopy achieves nanometer spatial resolution by localizing single fluorophores separated in space and time. A major challenge of single-molecule is the long acquisition time, leading to low throughput, as well a poor temporal that limits its use visualize dynamics cellular structures live cells. Another photobleaching, which reduces information density over time throughput available observation live-cell applications. To address both challenges, we combine...
The development of super-resolution microscopy (SRM) has widened our understanding biomolecular structure and function in biological materials. Imaging multiple targets within a single area would elucidate their spatial localization relative to the cell matrix neighboring biomolecules, revealing multi-protein macromolecular structures functional co-dependencies. SRM methods are, however, limited number suitable fluorophores that can be imaged during acquisition as well loss antigens antibody...
Single-molecule localization microscopy (SMLM) in combination with DNA barcoding (DNA-PAINT) enables easy-to-implement multi-target super-resolution imaging. However, image acquisition is slow because of the need to spatio-temporally isolate single emitters and collect sufficient statistical data generate a super-resolved image. Here, we bypass this limitation by utilizing neural network, DeepSTORM, that can predict SMLM images from high-emitter density data. This reduces time 10- 20-fold,...
Fluorophore labels that transiently and repetitively bind to a target ("exchangeable" or "renewable" labels) lead continuous renewal of the fluorescence signal. This dynamic labeling approach minimizes photobleaching was beneficially exploited in various super-resolution microscopy methods. Here, we report two new developments using exchangeable fluorophores: first, fast long-time live-cell weak-affinity protein label neural network. Second, novel design for DNA show higher brightness lower...
Abstract Stochastic optical reconstruction microscopy (STORM) is one of the most commonly used super-resolution techniques. Popular implementations STORM utilize aromatic fluorophores and consist a number intrinsic limitations such finite photostability dyes, reliance upon non-physiological redox buffers speed which ultimately limited by ‘off’-rates photoblinking. Self-activated nanodiamond-based (sandSTORM) has been developed as an accelerated protocol harvests rapid, high quantum-yield...
Abstract High Silica/phenolic composites are used for high performance applications, due to their strength and temperature resistant properties: however, they pose considerable problems while machining attain near net shape. This paper presents a study of thermal effects occurring during face turning such composites. The at the cutting zone was measured by an infrared pyrometer. A critical range have been identified which, specific pressure is almost steady. Modelling using log linear...
ABSTRACT The biocompatibility of surface coated/functionalised magnetic nanoparticles (MNPs) is key to their successful incorporation and application in biological systems. Polyethylene imine (PEI) -coated MNPs provide improved vitro transfection efficiency compared conventional chemical methods such as Lipofectamine cationic polymers, are also safer than viral transduction. Commercial cell toxicity assays useful for end-point high-throughput screening, providing fast results an overview...
The biocompatibility of surface-coated magnetic nanoparticles (MNPs) is key to their successful use in biomedical applications. Polyethyleneimine-coated MNPs (MNP-PEIs) provide improved vitro nucleic acid transfection efficiency and are safer compared conventional chemicals. Commercial cell toxicity assays useful for end-point high-throughput screening, however, they only reports cells that have undergone an extreme toxic response leading death. Cell a complex process which can be expressed...