A5089700193- Advanced Proteomics Techniques and Applications
- Mass Spectrometry Techniques and Applications
- Advanced biosensing and bioanalysis techniques
- Glycosylation and Glycoproteins Research
- Extracellular vesicles in disease
- Analytical Chemistry and Chromatography
- Enzyme Structure and Function
- Peptidase Inhibition and Analysis
- Covalent Organic Framework Applications
- Pregnancy and preeclampsia studies
- Ubiquitin and proteasome pathways
- Biosensors and Analytical Detection
- Identification and Quantification in Food
- Biotin and Related Studies
- Metal-Organic Frameworks: Synthesis and Applications
- Molecular Sensors and Ion Detection
- Endoplasmic Reticulum Stress and Disease
- Luminescence and Fluorescent Materials
- Lipid Membrane Structure and Behavior
- Enzyme Production and Characterization
- Nanopore and Nanochannel Transport Studies
- Diatoms and Algae Research
- Nanoplatforms for cancer theranostics
- Microfluidic and Capillary Electrophoresis Applications
- Carbon and Quantum Dots Applications
University of California, Irvine
2022-2024
State Key Laboratory of Proteomics
2016-2021
Beijing Proteome Research Center
2014-2021
Beijing Institute of Technology
2016-2018
Beijing Radiation Center
2014-2017
Exosomes can be efficiently isolated in a short period of time by the specific interaction titanium dioxide with phosphate groups on surface phospholipid bilayer.
Protein glycosylations play important roles in various biological processes and the disease progression of organisms. The development specific enrichment materials strategies before mass spectrometric analysis was a prerequisite to glycoproteomic due difficulty caused by substoichiometric levels glycoproteins. In this work, novel magnetic covalent organic frameworks (denoted as Fe3O4@TpPa-1) were first developed using only two-step solvothermal reaction then applied hydrophilic...
Mass spectrometry (MS)-based glycoproteomics research requires highly efficient sample preparation to eliminate interference from non-glycopeptides and improve the efficiency of glycopeptide detection. In this work, a novel MoS2/Au-NP (gold nanoparticle)–L-cysteine nanocomposite was prepared for enrichment. The two-dimensional (2D) structured MoS2 nanosheets served as matrix that could provide large surface area immobilizing hydrophilic groups (such L-cysteine) with low steric hindrance...
Cross-linking mass spectrometry (XL-MS) is an emergent technology for studying protein–protein interactions (PPIs) and elucidating architectures of protein complexes. The development various MS-cleavable cross-linkers has facilitated the identification cross-linked peptides, enabling XL-MS studies at systems level. However, scope depth cellular networks revealed by current technologies remain limited. Due to inherently broad dynamic range complexity proteomes, interference from highly...
Protein–protein interactions (PPIs) are fundamental to understanding biological systems as protein complexes the active molecular modules critical for carrying out cellular functions. Dysfunctional PPIs have been associated with various diseases including cancer. Systems-wide PPI analysis not only sheds light on pathological mechanisms, but also represents a paradigm in identifying potential therapeutic targets. In recent years, cross-linking mass spectrometry (XL-MS) has emerged powerful...
Exosomes are small membrane-bound vesicles secreted by most cell types and play an important role in cell-to-cell communication. Increasing evidence shows that exosomal proteins urine may be used as novel biomarkers for certain diseases. Purified urinary exosomes necessary downstream studies application development. However, conventional methods exosome isolation enrichment technically challenging time-consuming. Poor specificity, low recovery instrumental dependence also limit the use of...
The development of MS-cleavable cross-linking mass spectrometry (XL-MS) has enabled the effective capture and identification endogenous protein-protein interactions (PPIs) their residue contacts at global scale without cell engineering. So far, only lysine-reactive cross-linkers have been successfully applied for proteome-wide PPI profiling. However, lysine alone cannot uncover complete map in cells. Previously, we developed a maleimide-based cysteine-reactive cross-linker (bismaleimide...
The low abundance of glycopeptides in biological samples makes it necessary to enrich them before further analysis. In this study, the polymeric hydrophilic ionic liquid-modified magnetic (Fe3O4@MPS@PMAC) nanoparticles were synthesized via a one-step reflux-precipitation polymerization. Owing excellent hydrophilicity and strong electrostatic interaction toward polymerized liquid, [2-(methacryloyloxy) ethyl] trimethylammonium chloride (MAC), Fe3O4@MPS@PMAC exhibited outstanding performance...
Uterine artery (UA) hydrogen sulfide (H2S) production is augmented in pregnancy and, on stimulation by systemic/local vasodilators, contributes to pregnancy-dependent uterine vasodilation; however, how H2S exploits this role largely unknown. S-sulfhydration converts free thiols persulfides at reactive cysteine(s) targeted proteins affect the entire proteome posttranslationally, representing main route for elicit its function. Here, we used Tag-Switch quantify changes sulfhydrated (SSH-) (ie,...
Highly selective and sensitive enrichment for global phosphopeptides by novel magnetic nanoparticles Fe<sub>3</sub>O<sub>4</sub>@TCPP-DOTA-Ms.
Due to its key roles in regulating the occurrence and development of cancer, protein histidine phosphorylation has been increasingly recognized as an important form post-translational modification recent years. However, large-scale analysis is much more challenging than that serine/threonine or tyrosine phosphorylation, mainly because acid lability. In this study, MoS2–Ti4+ nanomaterials were synthesized using a solvothermal method taking advantage electrostatic adsorption between MoS2...
A key step in the workflow of bottom-up proteomics is proteolysis proteins into peptides with trypsin. In addition, enzyme-catalytic (18)O labeled as internal standards coupled multiple reaction monitoring mass spectrometry (MRM MS) for absolute quantitation target proteome commonly used its convenient operation and low cost. However, long digestion labeling times, incomplete to (16)O back exchange limit application, therefore, we developed a rapid efficient method based on high ratio...
A new type of mixed lanthanides-immobilized (Tb(3+) , Tm(3+) Ho(3+) Lu(3+) ) magnetic nanoparticles, Fe3 O4 @TCPP-DOTA-M(3+) was prepared with a particle size approximately 30 nm. model protein, α-casein, and protein mixture α-casein BSA (1:100) were first used to test the phosphopeptide enrichment efficiency newly developed nanoparticles. For 19 phosphopeptides identified materials. Even in tryptic digest (1:100), 16 easily detected, suggesting that novel materials possess high selectivity...
A novel microscale preparative gel electrophoresis system is designed and manufactured for protein separation preparation.
The synthesis process of Fe<sub>3</sub>O<sub>4</sub>@TMOS, a surfactant for the pretreatment complex biological samples.