A5053099063- Antibiotic Resistance in Bacteria
- Microbial infections and disease research
- Burkholderia infections and melioidosis
- Bacterial Genetics and Biotechnology
- Bacterial biofilms and quorum sensing
- Streptococcal Infections and Treatments
- Blood groups and transfusion
- Bacterial Identification and Susceptibility Testing
- Infections and bacterial resistance
- Vibrio bacteria research studies
- Escherichia coli research studies
- Bacteriophages and microbial interactions
- Galectins and Cancer Biology
- Brucella: diagnosis, epidemiology, treatment
- Herpesvirus Infections and Treatments
- Salmonella and Campylobacter epidemiology
- Research on Leishmaniasis Studies
- Antimicrobial Resistance in Staphylococcus
- RNA and protein synthesis mechanisms
- Reproductive tract infections research
- Aquaculture disease management and microbiota
- Metabolomics and Mass Spectrometry Studies
- Pneumonia and Respiratory Infections
- Advanced Proteomics Techniques and Applications
- Toxin Mechanisms and Immunotoxins
Universitat Autònoma de Barcelona
2011-2021
Barcelona Institute for Global Health
2015-2018
Universitat de Barcelona
2015-2018
Hospital Sant Joan de Déu Barcelona
2017
Hospital Clínic de Barcelona
2015
Czech Academy of Sciences, Institute of Biotechnology
2015
Beijing Chaoyang Emergency Medical Center
2010
Institut Químic de Sarrià
2001
Universitat Ramon Llull
2001
The pilus 2a backbone protein (BP-2a) is one of the most structurally and functionally characterized components a potential vaccine formulation against Group B Streptococcus. It by six main immunologically distinct allelic variants, each inducing variant-specific protection. To investigate molecular determinants driving variant immunogenic specificity BP-2a, in terms single residue contributions, we generated monoclonal antibodies specific based on their capability to recognize polymerized...
Mycoplasma genitalium is a human pathogen that mediates cell adhesion by complex structure known as the attachment organelle. This composed of cytadhesins and cytadherence-associated proteins, but few data are available about specific role these proteins in M. cytadherence. We have deleted homologous recombination mg191 mg192 genes from MgPa operon encoding P140 P110 cytadhesins. Molecular characterization mutants has revealed reciprocal posttranslational stabilization between two proteins....
Quorum Sensing (QS) mediated by Acyl Homoserine Lactone (AHL) molecules are probably the most widespread and studied among Gram-negative bacteria. Canonical AHL systems composed a synthase (LuxI family) regulator element (LuxR family), whose genes usually adjacent in genome. However, incomplete AHL-QS machinery lacking LuxI is frequently observed Proteobacteria, then referred as LuxR solo. It has been shown that certain solos participate interspecific communication detecting signals produced...
The main objective of this study was to investigate the relationship among in vivo acquisition antimicrobial resistance Pseudomonas aeruginosa clinical isolates, underlying molecular mechanisms and previous exposure antipseudomonal agents. PFGE used relatedness strains. MICs ceftazidime, cefepime, piperacillin/tazobactam, imipenem, meropenem, ciprofloxacin amikacin were determined. Outer membrane protein profiles assessed OprD expression. RT–PCR performed analyse ampC, mexB, mexD, mexF mexY...
We present an interdisciplinary approach that, by incorporating a range of experimental and computational techniques, allows the identification characterization functional/immunogenic domains. This has been applied to ArtJ, arginine-binding protein whose orthologs in Chlamydiae trachomatis (CT ArtJ) pneumoniae (CPn are shown have different immunogenic properties despite high sequence similarity (60% identity). solved crystallographic structures CT ArtJ CPn which found display type II...
Burkholderia pseudomallei is a Gram-negative bacterium responsible for melioidosis, serious and often fatal infectious disease that poorly controlled by existing treatments. Due to its inherent resistance the major antibiotic classes facultative intracellular pathogenicity, an effective vaccine would be extremely desirable, along with appropriate prevention therapeutic management. One of main subunit candidates flagellin (FliCBp). Here, we present high resolution crystal structure FliCBp...
Melioidosis, caused by the Gram-negative bacterium Burkholderia pseudomallei, is a potentially fatal infection that endemic in Southeast Asia and Northern Australia poorly controlled antibiotics. Research efforts to identify antigenic components for melioidosis vaccine have led identification of several proteins, including subunits forming flagella mediate bacterial motility, host colonization, virulence. This study focuses on B. pseudomallei flagellar hook-associated protein (FlgK(Bp)),...
Abstract In Pseudomonas aeruginosa , Ttg2D is the soluble periplasmic phospholipid-binding component of an ABC transport system thought to be involved in maintaining asymmetry outer membrane. Here we use crystallographic structure at 2.5 Å resolution reveal that this protein can accommodate four acyl chains. Analysis available structures orthologs shows they conform a new substrate-binding-protein structural cluster. Native and denaturing mass spectrometry experiments confirm Ttg2D, produced...
Stenotrophomonas maltophilia is a Gram-negative pathogen with emerging nosocomial incidence. Little known about its pathogenesis and the genomic diversity exhibited by clinical isolates complicates study of pathogenicity virulence factors. Here, we present strategy to identify such factors in new S. maltophilia, incorporating an adult-zebrafish model infection evaluate relative coupled 2D difference gel electrophoresis explore underlying differences protein expression. In this report upon...
The Streptomyces sp. beta-glucosidase (Bgl3) is a retaining glycosidase that belongs to family 1 glycosyl hydrolases. Steady-state kinetics with p-nitrophenyl beta-D-glycosides revealed the highest k(cat)/K(M) values are obtained glucoside (with strong substrate inhibition) and fucoside no substrates Bgl3 has 10-fold glucosidase over galactosidase activity. Reactivity studies by means of Hammett analysis using series substituted aryl beta-glucosides gave biphasic plot log k(cat) vs pK(a)...
Summary Isolation and characterization of transposon‐generated Mycoplasma genitalium gliding‐deficient mutants has implicated mg200 mg386 genes in gliding motility. The proposed role these was confirmed by restoration the phenotype deficient through gene complementation with their respective or wild‐type copies. are first reported gliding‐associated mycoplasma not directly involved cytadherence. Orthologues MG200 MG386 proteins also found slow mycoplasmas, pneumoniae gallisepticum ,...
Objectives: Acinetobacter nosocomialis has increasingly been reported as an opportunistic pathogen causing nosocomial infections.Although it is more susceptible to all antimicrobial agents than baumannii, MDR clinical isolates have also described.In addition, several studies shown a high percentage of resistance colistin.Therefore, in the present study we investigated mechanism colistin this microorganism.Methods: Colistin-resistant strains were selected from original colistin-susceptible A....
The terminal organelle is a differentiated structure that plays key role in mycoplasma cytadherence and locomotion. For this reason, the analysis of Mycoplasma genitalium mutants displaying anomalous organelles could improve our knowledge regarding structural elements required for proper In study, we isolated several M. having transposon insertions within mg218 or mg317 genes, which encode orthologues pneumoniae HMW2 HMW3 cytoskeletal proteins, respectively. As expected, mg218− mg317−...
In this study, we have characterized the functional properties of a novel Escherichia coli antigen named EsiB (E. secretory immunoglobulin A-binding protein), recently reported to protect mice from sepsis. Gene distribution analysis panel 267 strains representative different E. pathotypes revealed that esiB is preferentially associated with extraintestinal strains, while gene rarely found in either intestinal or nonpathogenic strains. These findings were supported by presence anti-EsiB...
Mycoplasma genitalium is a human pathogen associated with several sexually transmitted diseases. Proteomic technologies, along other methods for global gene expression analysis, play key role in understanding the mechanisms of bacterial pathogenesis and physiology. The proteome M. genitalium, model minimal cell, has been extended using combination different proteomic approaches technologies. total this microorganism analyzed gel-based gel-free approaches, achieving identification 85.3%...
Abstract A key issue towards developing new chemotherapeutic approaches to fight Mycobacterium tuberculosis is understand the mechanisms underlying drug resistance. Previous studies have shown that genes Rv1686c-Rv1687c and Rv3161c , predicted encode an ATP-binding cassette transporter a dioxygenase respectively, are induced in presence of triclosan other antimicrobial compounds. Therefore possible role resistance has been suggested for products these although no functional done. The aim...
Abstract Background Increasing rates of antimicrobial resistance among uropathogens led, other efforts, to the application subtractive reverse vaccinology for identification antigens present in extraintestinal pathogenic E. coli (ExPEC) strains but absent or variable non-pathogenic strains, a quest broadly protective Escherichia vaccine. The protein coded by locus c5321 from CFT073 was identified as one nine potential vaccine candidates against ExPEC and able confer protection with an...
A proteome map of Mycoplasma penetrans has been constructed using two-dimensional gel electrophoresis in combination with mass spectrometry (MS). infects the urogenital and respiratory tracts humans. total 207 spots were characterized MS and, comparing experimental data DNA sequence-derived predictions, it was possible to assign these 153 genes. The Pro-Q Diamond phosphoprotein dye technology used for fluorescent detection 26 phosphoproteins 4−7 pH range. Keywords: proteomics •...
Burkholderia pseudomallei is the etiological agent of melioidosis, a severe endemic disease in South-East Asia, causing septicemia and organ failure with high mortality rates. Current treatments diagnostic approaches are largely ineffective. The development new tools vaccines toward effective therapeutic opportunities against B. therefore an urgent priority. In framework multidisciplinary project tackling melioidosis through reverse structural vaccinology, BPSL1050 was identified as...