A5085761411- Antimicrobial Resistance in Staphylococcus
- Antibiotic Resistance in Bacteria
- Orthopedic Infections and Treatments
- Bacterial biofilms and quorum sensing
- Antimicrobial Peptides and Activities
- Orthopaedic implants and arthroplasty
- RNA and protein synthesis mechanisms
- Antibiotics Pharmacokinetics and Efficacy
- Viral Infectious Diseases and Gene Expression in Insects
- RNA Research and Splicing
- Infective Endocarditis Diagnosis and Management
- Bacterial Identification and Susceptibility Testing
- RNA modifications and cancer
- Pneumonia and Respiratory Infections
- Streptococcal Infections and Treatments
- Biochemical and Structural Characterization
- Surgical site infection prevention
- Bacterial Genetics and Biotechnology
- Infectious Diseases and Tuberculosis
- Heat shock proteins research
- Travel-related health issues
- Thyroid Cancer Diagnosis and Treatment
- Endodontics and Root Canal Treatments
- Nosocomial Infections in ICU
- Salmonella and Campylobacter epidemiology
Universitat de Barcelona
2008-2023
Institut d'Investigació Biomédica de Bellvitge
2013-2023
Bellvitge University Hospital
2011-2023
Ajuntament de L’Hospitalet
2018
Hospital Pablo Tobon Uribe
2018
Red Espanola de Investigacion en Patologia Infecciosa
2013-2015
Instituto de Salud Carlos III
2013
Hospital Clínic de Barcelona
2002-2011
Consorci Institut D'Investigacions Biomediques August Pi I Sunyer
2000-2010
University Hospital Complex Of Vigo
1997-2010
To determine the prevalence and incidence of hypopituitarism in general population.The study population comprised an average sample 146,000 adult inhabitants South Galicia (northwestern Spain). The Medical Register General Hospital Vigo ensured virtually complete case ascertainment for diagnosed this population. Only patients residing area were included. diagnosis was based on baseline hormonal dynamic tests.The two cross-sectional surveys, first from January to December 1992 second 1999,...
Identifying the etiologic microorganism is essential to guide antimicrobial therapy in prosthetic joint infection.We (1) compared frequency of positive cultures with synovial fluid inoculated blood culture flasks (SF) those periprosthetic tissues or swabs traditional from patients acute and chronic infections (PJI) (2) determined sensitivity, specificity, predictive values three methods.We retrospectively reviewed 87 PJIs (54 knees, 33 hips) 63 aseptic loosening (34 29 hips). Two SF, tissue,...
The aim of our study was to evaluate the outcome acute prosthetic joint infections (PJIs) due gram-negative bacilli (GNB) treated without implant removal. Patients with an PJI GNB diagnosed from 2000 2007 were prospectively registered. Demographics, comorbidity, type implant, microbiology data, surgical treatment, antimicrobial therapy, and recorded. Classification regression tree analysis, Kaplan-Meier survival method, Cox model applied. Forty-seven patients included. mean age 70.7 years,...
Ribosomal salt washes of Artemia salina embryos contain a protein(s) that binds [3H]m7GpppGpC and [3H]m7GpppGmpC, as measured by retention on nitrocellulose membrane filters. These oligonucleotides correspond in structure to the methylated 5'-terminal sequences (caps) present many eukaryotic mRNAs. The cap binding protein does not bind unmethylated counterparts caps, e.g., [32P]GpppGpCp, or derivative m7GpppGmpC containing ring-opened m7G. None purified initiation factors IF-MP, IF-M2A,...
Human Spα is a soluble protein belonging to group B of the scavenger receptor cysteine-rich (SRCR) superfamily for which little functional information available. It expressed by macrophages present in lymphoid tissues (spleen, lymph node, thymus, and bone marrow), it binds myelomonocytic cells, suggests that may play an important role regulation innate adaptive immune systems. In study we show recombinant human (rSpα) surface several Gram-positive Gram-negative bacterial strains. Competition...
The initiation inhibitor of reticulocyte lysates has been shown by others to be associated with a 3':5'-cyclic-AMP-independent protein kinase that catalyzes the phosphorylation small (38,000 daltons) subunit polypeptide chain factor eIF-2. This forms ternary complex Met-tRNAi and GTP which, on interaction 40S ribosome, gives rise complex. Ternary formation is inhibited prior incubation partially purified eIF-2 ATP. relation between inactivation indicated lack inhibition when ATP omitted....
The emergence of multidrug-resistant Pseudomonas aeruginosa infections has urged the need to find new strategies, such as use combinations antibiotics. Among these, combination colistin with other antibiotics been studied. Here, action and rifampicin on both planktonic sessile cells colistin-resistant P. was Dynamic biofilms were formed treated a combination, resulting in an active killing effect colistin-susceptible biofilms. results suggest that outer membrane facilitates penetration,...
Quinolone susceptibility was analyzed in 17 clinical isolates of Corynebacterium striatum and 9 strains amycolatum by the E-test method Mueller-Hinton agar plates. The C. ATCC 6940 strain used as a control strain. amplified quinolone resistance determining regions gyrA genes were characterized. Four vitro quinolone-resistant mutants selected analyzed. Both vivo showed high levels fluoroquinolone with double mutation leading to an amino acid change positions 87 91 or 88 (unusual mutation)...
We have studied the role of Drosophila 35-kDa cap-binding protein (CBP) and CBP complex in mechanism messenger RNA discrimination established heat-shocked embryos. is functionally equivalent to mammalian eucaryotic initiation factor (eIF)-4E complex, which includes eIF-4E, might be counterpart eIF-4F. By using anti-eIF-4E antibodies, we found that although translation bulk normal messengers lysates was very dependent on mRNAs for heat shock proteins (hsps) (particularly hsp70 mRNA with...
The activity of IF-MP, a polypeptide chain initiation factor that forms ternary complex with eukaryotic initiator Met-tRNA and GTP promotes binding the to 40S ribosomes, is very low in undeveloped Artemia salina embryos but increases over 20-fold following resumption development upon hydration cysts. present both ribosomal salt wash high-speed-supernatant. Its specific 50 times higher its total only about twice as high supernatant. As true IF-MP from other sources, A. for Met-tRNAi sensitive...
Methicillin-resistant Staphylococcus aureus (MRSA) ST398, associated with livestock animals, was described in 2003 as a new lineage infecting or colonizing humans. We evaluated the prevalence and molecular characteristics of MRSA ST398 isolated Hospital Universitari de Bellvitge from January 2000 to June 2011. Tetracycline resistant (Tet-R) isolates single patients (pts) were screened by SmaI-pulsed field gel electrophoresis (PFGE). Nontypable strains SmaI (NTSmaI)-MRSA further analysed...
Despite its use was abandoned several decades ago, the polycationic peptide colistin has become last hope to treat severe infections caused by multidrug-resistant Gram-negative bacteria. Thus, development of resistance may seriously compromise efficacy treatment. Moreover, high toxicity being dose dependent. A potentially effective strategy avoid be combine with other antimicrobials. This help in rescue old antimicrobials and reducing toxic undesired effects.Antimicrobial susceptibility...
The formation of translational inhibitor (active eIF-2 kinase) from proinhibitor (inactive in reticulocyte lysates, known to be controlled by hemin, can, as we recently reported, induced 3':5'-cyclic AMP(cAMP)-dependent protein kinase (ATP:protein phosphotransferase, EC 2.7.1.37) or its catalytic subunit. We find that crude preparations rabbit hemin inhibits the conversion catalyzed endogenous cAMP-dependent upon addition cAMP, but not caused free Hemin prevents binding cAMP regulatory...
Objective: To evaluate the effects of systemic treatment with linezolid compared vancomycin on biofilm formation in mechanically ventilated pigs severe methicillin-resistant Staphylococcus aureus–induced pneumonia. Design: Prospective randomized animal study. Setting: Departments Pneumology, Microbiology, and Pharmacy Hospital Clínic, Barcelona, Scientific Technological Services University Barcelona. Subjects: We prospectively analyzed 70 endotracheal tube samples. Endotracheal tubes were...