A5019973364- Alzheimer's disease research and treatments
- Ubiquitin and proteasome pathways
- Endoplasmic Reticulum Stress and Disease
- Protein Structure and Dynamics
- Cholinesterase and Neurodegenerative Diseases
- Chemical Synthesis and Analysis
- Peptidase Inhibition and Analysis
- Computational Drug Discovery Methods
- Angiogenesis and VEGF in Cancer
- Enzyme Structure and Function
- Microtubule and mitosis dynamics
- Microbial Metabolic Engineering and Bioproduction
- Electrochemical sensors and biosensors
- Genetics and Neurodevelopmental Disorders
- Cancer Mechanisms and Therapy
- Graphene and Nanomaterials Applications
- Axon Guidance and Neuronal Signaling
- Carbon and Quantum Dots Applications
- Trace Elements in Health
- Carbohydrate Chemistry and Synthesis
- RNA Interference and Gene Delivery
- Polymer composites and self-healing
- RNA and protein synthesis mechanisms
- Fluorine in Organic Chemistry
- Click Chemistry and Applications
Institute of Crystallography
2019-2022
Universidad de Zaragoza
2016-2022
Consorzio Catania Ricerche
2020-2022
Instituto de Nanociencia y Materiales de Aragón
2022
Institute of Biostructure and Bioimaging
2018-2019
University of Catania
2019
National Research Council
2018
Instituto de Síntesis Química y Catálisis Homogénea
2016
An increasing number of human diseases has been shown to be linked aggregation and amyloid formation by intrinsically disordered proteins (IDPs). Amylin, amyloid-β, α-synuclein are, indeed, involved in type-II diabetes, Alzheimer's, Parkinson's, respectively. Despite the correlation toxicity these at early stages with membrane damage, molecular events underlying process is quite complex understand. In this study, we demonstrate crucial role free lipids lipid–protein complex, which enables an...
Appetite for ubiquitin: a gushy travel companion in the intracellular journey of amyloid β peptide.
Type 2 Diabetes is a major public health threat, and its prevalence increasing worldwide. The abnormal accumulation of islet amyloid polypeptide (IAPP) in pancreatic β-cells associated with the onset disease. Therefore, design small molecules able to inhibit IAPP aggregation represents promising strategy development new therapies. Here we employ vitro, biophysical, computational methods inspect ability Silybin A B, two natural diastereoisomers extracted from milk thistle, interfere toxic...
Alzheimer's disease (AD) is linked to the abnormal accumulation of amyloid β peptide (Aβ) aggregates in brain. Silybin B, a natural compound extracted from milk thistle (Silybum marianum), has been shown significantly inhibit Aβ aggregation vitro and exert neuroprotective properties vivo. However, further explorations silybin B's clinical potential are currently limited by three main factors: (a) poor solubility, (b) instability blood serum, (c) only partial knowledge silybin's mechanism...
Proteasome malfunction parallels abnormal amyloid accumulation in Alzheimer's Disease (AD). Here we scrutinize a small library of pyrazolones by assaying their ability to enhance proteasome activity and protect neuronal cells from toxicity. Tube tests evidenced that aminopyrine nifenazone behave as 20S activators. Enzyme assays carried out on an "open gate" mutant (α3ΔN) demonstrated activates through binding the α-ring surfaces influencing gating dynamics. Docking studies coupled with...
The structural effects of zinc replacement by xenobiotic metal ions have been widely studied in several eukaryotic and prokaryotic zinc-finger-containing proteins. finger, that presents a bigger βββαα domain with larger hydrophobic core respect to its counterpart, represents valuable model protein study ion interaction metallo-proteins. Several studies conducted on Ros87, the DNA binding finger Ros, demonstrated appears structurally tolerate Ni(II), albeit important perturbations, but not...
n-Eicosane-melamine formaldehyde microcapsules of an average size 1.1 μm and latent heat fusion 146.2 ± 5.3 J/g have been prepared. They characterized by scanning electron microscopy, FTIR spectroscopy, calorimetric techniques, thermogravimetric analyses. Under processing conditions, the apparently preserved their properties, also maintaining n-eicosane loading storage capacity under washing conditions (water with detergent at 60 °C). The synthesis has scaled up for fabrication functional...
Abstract Alzheimer's disease (AD) is a multifactorial pathology that requires multifaceted agents able to address its peculiar nature. Increasing evidence has shown aggregation of amyloid β (Aβ) and oxidative stress are strictly interconnected, their modulation might have positive synergic effect in contrasting AD‐related impairments. Herein, new efficient fragment‐based approach towards tyrosol phosphodiester derivatives (TPDs) been developed starting from suitable building blocks...
Four specifically designed IDE mutants have been used to unveil the molecular basis of peptidase <italic>versus</italic> E1-like activity enzyme.
Abstract Mature microRNAs are short non-coding RNA sequences which upon incorporation into the RISC ribonucleoprotein complex, play a crucial role in regulation of gene expression. However, miRNAs can exist within cell also as free molecules fulfilling their biological activity. Therefore, it is emerging that addition to sequence even structure adopted by mature might an important reach target. Indeed, we analysed several spectroscopic techniques secondary structures two artificial selected...
Ethynylation and propargylation of chiral nonracemic polyhydroxylated cyclic nitrones with Grignard reagents are efficient methods for preparing building blocks containing an alkyne moiety to be used in copper-catalyzed azide cycloaddition click chemistry. Whereas ethynylation takes place excellent diastereoselectivity, afforded mixtures diastereomers some cases. The use (trimethylsilyl)propargyl bromide as precursor the reagent is necessary avoid formation undesired allene derivatives. DFT...
This review covers the recent advances in synthesis of enantiomerically pure hydroxylamine's employing nitrones as starting materials. Nucleophilic additions organometallic reagents to are most common way for introducing a hydroxyamino group into carbon skeletons with concomitant formation new carbon-carbon bond. Addition nucleophiles derived from enolates, cyanide or fluorinated derivatives allows preparation complex structures. Radical and, particular samarium diiodidemediated reductive...
Copper plays an important role as a regulator in many pathologies involving the angiogenesis process. In cancerogenesis, tumor progression, and angiogenic diseases, copper homeostasis is altered. Although details pathways involved are still unknown, some copper-specific ligands have been successfully used therapeutic agents. Copper-binding peptides able to modulate represent possible way value new drugs. We previously reported that fragment (VEGF73-101) of vascular endothelial growth factor...
Abstract Downhill folding has been defined as a unique thermodynamic process involving conformations ensemble that progressively loses structure with the decrease of protein stability. folders are estimated to be rather rare in nature they miss an energetically substantial barrier can protect against aggregation and proteolysis. We have previously demonstrated prokaryotic zinc finger Ros87 shows bipartite folding/unfolding which metal binding intermediate converts native through delicate...
Increasing number of human diseases have been shown to be linked aggregation and amyloid formation by intrinsically disordered proteins (IDPs). Amylin, amyloid-β, α-synuclein are, indeed, involved in type-II diabetes, Alzheimer’s, Parkinson’s, respectively. Despite the correlation toxicity these at early stages with membrane damage, molecular events underlying process is quite complex understand. In this study, we demonstrate crucial role free lipids lipid-protein complex, which enables an...
<p>Increasing number of human diseases have been shown to be linked aggregation and amyloid formation by intrinsically disordered proteins (IDPs). Amylin, amyloid-β, α-synuclein are, indeed, involved in type-II diabetes, Alzheimer’s, Parkinson’s, respectively. Despite the correlation toxicity these at early stages with membrane damage, molecular events underlying process is quite complex understand. In this study, we demonstrate crucial role free lipids lipid-protein complex, which...
Folding stability is a crucial feature of protein evolution and essential for functions. Thus, the comprehension folding mechanisms represents an important complement to structure function, determine structural basis misfolding. In this context, thermal unfolding studies represent useful tool get molecular description conformational transitions governing folding/unfolding equilibrium given protein. Here, we report pathway VEGFR1D2, member immunoglobulin superfamily by means high-resolution...