A5031248964- DNA and Nucleic Acid Chemistry
- Advanced biosensing and bioanalysis techniques
- RNA Interference and Gene Delivery
- Silymarin and Mushroom Poisoning
- Pharmaceutical and Antibiotic Environmental Impacts
- Carbohydrate Chemistry and Synthesis
- Chemical Synthesis and Analysis
- Synthesis and bioactivity of alkaloids
- Natural product bioactivities and synthesis
- Phytochemistry and Biological Activities
- Analytical chemistry methods development
- Berberine and alkaloids research
- Biological and pharmacological studies of plants
- Drug-Induced Hepatotoxicity and Protection
- Plant and animal studies
- HIV/AIDS drug development and treatment
- Calcium signaling and nucleotide metabolism
- Cholinesterase and Neurodegenerative Diseases
- Endoplasmic Reticulum Stress and Disease
- Water Treatment and Disinfection
- Natural Antidiabetic Agents Studies
- RNA and protein synthesis mechanisms
- Alzheimer's disease research and treatments
- Antibiotics Pharmacokinetics and Efficacy
- Environmental Toxicology and Ecotoxicology
University of Naples Federico II
2016-2025
University of L'Aquila
2024
University of Catania
2023
Federico II University Hospital
2015-2022
Associazione Italiana Vulvodinia Onlus
2022
Istituto Nazionale di Fisica Nucleare, Sezione di Napoli
2015
University of Sannio
2014
Rega Institute for Medical Research
2008
KU Leuven
2008
Institute of Protein Biochemistry
2006
Methylation of adenine N6 (m6A) is the most frequent RNA modification. On mRNA, it catalyzed by METTL3–14 heterodimer complex, which plays a key role in acute myeloid leukemia (AML) and other types blood cancers solid tumors. Here, we disclose first proteolysis targeting chimeras (PROTACs) for an epitranscriptomics protein. For designing PROTACs, made use crystal structure complex with potent selective small-molecule inhibitor (called UZH2). The optimization linker started from desfluoro...
The self-assembling of the amyloid β (Aβ) peptide into neurotoxic aggregates is considered a central event in pathogenesis Alzheimer's disease (AD). Based on "amyloid hypothesis", many efforts have been devoted to designing molecules able halt progression by inhibiting Aβ self-assembly. Here, we combine biophysical (ThT assays, TEM and AFM imaging), biochemical (WB ESI-MS), computational (all-atom molecular dynamics) techniques investigate capacity four optically pure components natural...
Beer is one of the most consumed alcoholic beverages in world, rich chemical compounds natural origin with high nutritional and biological value. It made up water, barley malt, hops, yeast. The main nutrients are carbohydrates, amino acids, minerals, vitamins, other such as polyphenols which responsible for many health benefits associated this consumption drinks. Hops malt raw materials beer a source phenolic compounds. In fact, about 30% comes from hops 70%-80% malt. Natural foods or plants...
High fat and/or carbohydrate intake are associated with an elevated risk for obesity and chronic diseases such as diabetes cardiovascular diseases. The harmful effects of a high diet could be different, depending on dietary quality. In fact, diets rich in unsaturated fatty acids considered less deleterious human health than those saturated fat. our previous studies, we have shown that rats fed developed exhibited decrease oxidative capacity increase stress liver mitochondria. To investigate...
Type 2 Diabetes is a major public health threat, and its prevalence increasing worldwide. The abnormal accumulation of islet amyloid polypeptide (IAPP) in pancreatic β-cells associated with the onset disease. Therefore, design small molecules able to inhibit IAPP aggregation represents promising strategy development new therapies. Here we employ vitro, biophysical, computational methods inspect ability Silybin A B, two natural diastereoisomers extracted from milk thistle, interfere toxic...
Patulin is a toxic secondary metabolite of number fungal species belonging to the genera Penicillum and Aspergillus. It has been mainly isolated from apples apple products contaminated with common storage-rot fungus apples, expansum, but it also extracted rotten fruits, moldy feeds, stored cheese. Human exposure patulin can lead serious health problems, according long-term investigation in rats, World Health Organization set tolerable weekly intake 7 ppb body weight. The content foods...
Numerous substances from different chemical sectors, the pharmaceutical industry to many consumer products available for everyday usage, can find their way into water intended human consumption and wastewater, have adverse effects on environment health. Thus, disinfection process is an essential stage in wastewater treatment plants destroy pathogenic microorganisms but it form degradation byproducts. Sodium hypochlorite most common disinfectant, important drawback associated with this kind...
We investigate the therapeutic potential of Aloin A and B, two natural compounds derived from Aloe vera leaves, focusing on their neuroprotective anticancer properties. The structural differences between these epimers suggest that they may exhibit distinct pharmacological Our investigations revealed both are not stable in aqueous solution tend to degrade rapidly, with concentration decreasing by over 50% within approximately 12 h. These results underscore importance addressing issues such as...
Oligodeoxyribonucleotides of sequence d(5'TGGGAG3') carrying bulky aromatic groups at the 5' end were found to exhibit potent anti-HIV activity [Hotoda, H., et al. (1998) J. Med. Chem. 41, 3655-3663 and references therein]. Structure-activity relationship investigations indicated that G-quadruplex formation, as well presence large substituents 5'-end, both essential for their antiviral activity. In this work, we synthesized some representative examples active Hotoda's 6-mers analyzed...
Novel hybrid oligonucleotides carrying the G-quadruplex-forming d(5′TGGGAG3′) sequence, conjugated with mono- or disaccharides at 3′ 5′-end through phosphodiester bonds, have been synthesized as potential anti-HIV agents, via a fully automated, online phosphoramidite-based solid-phase strategy. CD-monitored thermal denaturation studies on resulting quadruplexes indicated insertion of single monosaccharide 3′-end optimal modification, conferring improved stability to quadruplex complex. In...
A series of d((5')TGGGAG(3')) sequences, 5'-conjugated with a variety aromatic groups through phosphodiester linkages, were synthesized, showing CD spectra diagnostic parallel-stranded, tetramolecular G-quadruplex structures. When tested for anti-HIV-1 and HIV-2 activity, potent inhibition HIV-1 infection in CEM cell cultures was found, associated high selectivity index values. Surface Plasmon Resonance assays revealed specific binding to gp120 gp41.
Silybin A and B were separated from commercial silibinin using the preparative HPLC method. The described method is rapid effective in obtaining gram-scale amounts of two diastereoisomers with minimal effort. In our approach, was dissolved THF (solubility greater than 100 mg/mL), an alternative solvent to H₂O or MeOH which has a very low solubility (ca 0.05-1.5 then into its components RP-HPLC. By starting purified diastereoisomers, it possible obtain enantiomers 2,3-dehydrosilybin good...
Alzheimer's disease (AD) is linked to the abnormal accumulation of amyloid β peptide (Aβ) aggregates in brain. Silybin B, a natural compound extracted from milk thistle (Silybum marianum), has been shown significantly inhibit Aβ aggregation vitro and exert neuroprotective properties vivo. However, further explorations silybin B's clinical potential are currently limited by three main factors: (a) poor solubility, (b) instability blood serum, (c) only partial knowledge silybin's mechanism...
Rationale:Silybum marianum is used to protect against degenerative liver damage. The molecular mechanisms of its bioactive component, silybin, remained enigmatic, although membrane-stabilizing properties, modulation membrane protein function, and metabolic regulation have been discussed for decades. Methods: Experiments were performed with hepatocyte cell lines primary monocytes in vitro under both basal stressed conditions, mice vivo. Quantitative lipidomics was detect changes phospholipids...
Curcumin is recognized for its diverse biological activities, including the ability to induce apoptosis and ferroptosis. Therefore, it represents a promising candidate development of new compounds with neuroprotective anticancer properties. In order synthesize mimics improved pharmacokinetic properties (better solubility stability than curcumin) here, we present design synthesis novel curcumin analogues named Ethylphosphonate-based (EPs), which preserve pharmacophoric features curcumin. New...