The aim of this study was to evaluate the cellulase and xylanase activity fungi isolated from digestive tract sheep effects supplementation with two selected on intake, digestibility, microbial protein (MP) synthesis nitrogen balance in lambs. first experiment evaluated carboxymethylcellulase (CMCase) activities filamentous yeasts, which were grown a culture medium containing Urochloa decumbens hay. second performed twenty-one male Santa Inês x Dorper crossbred lambs, initial body weight...

The objective of this study was to evaluate the aerobic microbiota rumen fluid from Holstein cows and heifers fed different tropical forage in north Minas Gerais, Brazil. A total 30 samples with sorghum silage were collected: 32 Brachiaria brizantha, 12 that received 11 calves sugar cane foliage. culture carried out using agar Sabouraud medium solid C medium, containing microcrystalline cellulose. isolated mycelial fungi identified by microculture technique yeasts micromorphological...

Abstract. Duarte ER, Lima SM, Nere JC, Freitas CES, Maia HAR, Júlio ADL, Abrão FO, Alves JMS, Santos VLD, Geraseev LC, Cota J. 2024. Yeasts isolated from bovine rumen selected to degrade lignocellulosic roughage. Biodiversitas 25: 1159-1166. Commercial yeast strains are viable alternatives chemical additives in ruminants; however, they not self the ecosystem. The present study was conducted compare degradation of Urochloa decumbens (Stapf) R.D.Webster (UD) hay and sugarcane bagasse (SB) by...

Este trabalho teve como objetivo avaliar o efeito da aplicação de lodo esgoto e silicato cálcio magnésio sobre os atributos físicos um Cambissolo Háplico, cultivado com girassol. Os tratamentos foram distribuídos em esquema fatorial (4 x 2) + 1, correspondendo a quatro doses desidratado (0; 6,25; 12,5 18,5 t ha-1), duas (0 240 g m-1) tratamento adicional adubação mineral. O delineamento utilizado foi blocos casualizados, três repetições. Após 150 dias adubação, coletaram-se amostras solos,...

Protozoários do rúmen podem representar 40% nitrogênio total e 60% produto final da fermentação apresentam atividade celulolítica. Neste estudo, objetivou-se quantificar identificar as populações de protozoários no novilhos, criados exclusivamente em pastagem, durante o período seco ano, Norte Minas Gerais. Amostras suco ruminal 36 novilhos mestiços Nelore foram coletadas diretamente rúmen, após abate, inicio, meio ou fim estiagem. Para conservação dos protozoários, 1 mL foi diluído 9...

Em diferentes paises em todo o mundo, tem-se caracterizado a microbiota ruminal e registrado importante participacao dos fungos na digestao no equilibrio do ecossistema saude ruminantes. Entretanto, pouco se tem estudado sobre caracterizacao de aerobios animais criados condicoes extensivas, pastagens baixo valor nutricional, frequentemente lignificadas. Pesquisas relacionadas ao ambiente nessas circunstâncias permitiriam selecao isolados fungicos com atividade superior enzimas celuloliticas...

Fungi populations were evaluated in large intestine from sheep raised the North of Minas Gerais, Brazil. The samples 39 Santa Ines crossbred ewes and 30 lambs on Tanzania grass (Panicum maximum cv. Tanzania), both supplemented with mineral mixtures. clinical specimens directly collected rectal ampoule sterile swabs. development fungal mycelium was observed all lamb positive cultures 34 ewe (87.2%). After microculture 40 colonies lambs, isolates genus Aspergillus, three Paecilomyces spp., one...

Abstract Commercial yeast has been frequently employed to improve the digestibility of ruminant diets, albeit these microorganisms are not indigenous rumen microbiome. In this investigation, our aim was assess in vitro Urochloa decumbens hay (UDH) with incorporation two selected strains isolated ruminal fluid Zebu cattle. Rumen collected at onset, midpoint, and final dry period a semiarid region Brazil. UDH samples were obtained from same region. Within simulated chambers, we included...

The objective with this study was to evaluate intake, feeding behavior, rumen fluid characteristics, performance, and nutrient digestibility of sheep fed banana pseudostem hay (BPH) without virginiamycin (VM). Thirty-two uncastrated male crossbred Dorper × Santa Inês lambs at approximately five months age, an average initial body weight 25.00±1.95 kg, were used in the experiment. Four diets tested, as follows: Tifton grass (TGH) plus concentrate VM; BPH replacing 60% TGH [...]

The objective of this review was to survey the main microbial species producing enzymes involved in degradation compounds such as cellulose, hemicellulose and lignin. Most microorganisms isolated from soil, waste compost material are capable a broad spectrum degrading plant cell wall. This enzymatic activity is essential for fermentation carbohydrates ruminal environment, since ruminants not able degrade these polymers, thus establishing symbiosis relationship between animals autochthonous...

The addition of cellulolytic fungi, or their enzymes, in diets containing high levels fiber are promising strategy for improving the performance. In this study, aims were to select fungi from digestive tract sheep fed different concentrations banana leaf hay. Thirty lambs raised a feedlot evaluated, distributed completely randomized design, with 0, 125, 250, 375, 500 g/Kg dry matter and six replications. Approximately 15 mL ruminal fluid swabs rectal ampulla collected. cultures carried out...

O ambiente ruminal é composto por inúmeros microrganismos, onde cada um deles possui papel fundamental no aproveitamento das dietas ingeridas pelos ruminantes, os fungos são seres unicelurares que atuam degradando as porções fibrosas dessa dieta. Objetivou-se neste estudo avaliar a atividade celulolítica (AC) de micelianos aeróbios provenientes do trato digestório ovinos. Foram identificados e avaliados 38 isolados borregos ovelhas, após o reisolamento, amostras desses microrganismos foram...

The objective of this study was to evaluate carcass yield, measurements, cuts, and fat deposition sheep fed banana pseudostem hay Tifton 85 grass hay, with or without virginiamycin. Thirty-three uncastrated male Dorper × Santa Ines crossbred lambs at five months age, an average body weight 25.00 ± 1.95 kg, were used in a feedlot experiment. following four diets evaluated: plus concentrate virginiamycin, replacing 60% experiment set completely randomized design, factorial scheme, using...

Abstract The authors have withdrawn this preprint due to a need make corrections.

Abstract BackgroundCommercial yeast strains are a promising alternative to chemical additives in ruminants; but not self the rumen ecosystem. In this study, aim was identify and evaluate growth, mass production, enzymatic activity of naturally isolated from bovine submerged fermentations containing roughages with high concentration lignocellulose. An experiment initially conducted compare degradation Urochloa decumbens (UD) hay or sugarcane bagasse (SB) by eight isolates control without...

Objetivou-se avaliar o desempenho da adição de uma cepa levedura isolada do líquido ruminal ovino hígido na dieta cordeiros desmamados e alimentados com feno Urochloa decumbens. No estudo, foram utilizados doze cordeiros, Santa Inês x Dorper, recém-desmamados. Os animais passaram por um período adaptação referente às instalações (15 dias) dois períodos experimentação (42 dias). O experimento foi conduzido em parcelas subdivididas, para grupo recebendo a outro controle meio cultivo sem...