A5053340529- Heat shock proteins research
- Phytochemicals and Medicinal Plants
- Genetics, Aging, and Longevity in Model Organisms
- Cancer-related Molecular Pathways
- Telomeres, Telomerase, and Senescence
- Medicinal Plants and Neuroprotection
- Cell death mechanisms and regulation
- DNA Repair Mechanisms
- PARP inhibition in cancer therapy
- Endoplasmic Reticulum Stress and Disease
- Mitochondrial Function and Pathology
- RNA Interference and Gene Delivery
- Spaceflight effects on biology
- Cancer, Hypoxia, and Metabolism
- Bee Products Chemical Analysis
- RNA modifications and cancer
- Epigenetics and DNA Methylation
- Cancer Research and Treatments
- thermodynamics and calorimetric analyses
- Computational Drug Discovery Methods
- Adipose Tissue and Metabolism
- Ubiquitin and proteasome pathways
- SARS-CoV-2 and COVID-19 Research
- Retinoids in leukemia and cellular processes
- RNA Research and Splicing
National Institute of Advanced Industrial Science and Technology
2016-2025
National Institutes of Health
2000-2023
National Cancer Institute
2010-2023
Care India
2020
University of Tsukuba
2011-2020
HAW Hamburg
2020
Indraprastha Apollo Hospitals
2009-2019
Children's Medical Research Institute
1995-2011
Medical College of Wisconsin
2011
Leiden University Medical Center
2011
Coronavirus disease 2019 (COVID-19) initiated in December Wuhan, China and became pandemic causing high fatality disrupted normal life calling world almost to a halt. Causative agent is novel coronavirus called Severe Acute Respiratory Syndrome 2 (SARS-CoV-2/2019-nCoV). While new line of drug/vaccine development has been world-wide, the current scenario infected numbers, severity morbidity, repurposing existing drugs heavily explored. Here, we used homology-based structural model...
The recent novel coronavirus, Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2/2019-nCoV) has caused a large number of deaths around the globe. There is an urgent need to understand this new virus and develop prophylactic therapeutic drugs. Since drug development expensive, intense time-consuming path, timely repurposing existing drugs often explored wherein research avenues including genomics, bioinformatics, molecular modeling approaches offer valuable strengths. Here, we have...
Teruaki Nomura, Md Matiullah Khan, Sunil C. Kaul, Hai-Dong Dong, Renu Wadhwa, Clemencia Colmenares, Isao Kohno, and Shunsuke Ishii Laboratory of Molecular Genetics, Tsukuba Life Science Center, RIKEN, Tsukuba, Ibaraki 305-0074, Japan; National Institute Bioscience Human Technology, Agency Industrial 305-0046, Chugai Research for Medicine, Niihari, 300-4101, Department Cancer Biology, Lerner Institute, Cleveland Clinic Foundation, Cleveland, Ohio 44195 USA; Iatron Laboratories Inc., Tako,...
Abstract Mortalin, also known as mthsp70/GRP75/PBP74, interacts with the tumor suppressor protein p53 and inactivates its transcriptional activation apoptotic functions. Here, we examined level of mortalin expression in a large variety tissues, tumor‐derived vitro immortalized human cells. It was elevated many tumors, all In embryonic fibroblasts an plasmid for hTERT, telomerase catalytic subunit, or without papillomavirus E6 E7 genes, found that subclones spontaneously increased levels...
The mortalin genes, <i>mot-1</i> and <i>mot-2</i>, are hsp70 family members that were originally cloned from normal immortal murine cells, respectively. Their proteins differ by only two amino acid residues but exhibit different subcellular localizations, arise distinct have contrasting biological activities. We report here the also in their interactions with tumor suppressor protein p53. pancytosolic mot-1 cells did not show colocalization p53; contrast, nonpancytosolic mot-2 p53 overlapped...
DNA methylation is essential for development in the mouse and plays an important role inactivation of X chromosome genomic imprinting. MeCP2 founder member a family methyl-CpG-binding proteins. directly binds to co-repressor mSin3, which interacts with class I histone deacetylase, recruiting them methyl-CpG regions suppress transcription. Here, we report that two co-repressors, c-Ski N-CoR, addition mSin3A, c-Ski, encoded by c-<i>ski</i> proto-onocogene, required MeCP2-mediated...
A novel 66-kDa protein, named p66mot-1, is identified to be associated with cellular mortality by virtue of its presence in cytosolic fractions serially passaged mouse embryonic fibroblasts (MEF) and the mortal hybrids obtained fusion immortal (MN48-1, derivative NIH 3T3) cells. Immortal cells lack this protein their fractions. cDNA cloning homology search placed it heat shock 70 (hsp70) family. Microinjection anti-p66 antibody senescent MEF could transiently stimulate cell division, which...
Abstract Purpose: Ashwagandha is regarded as a wonder shrub of India and commonly used in Ayurvedic medicine health tonics that claim its variety health-promoting effects. Surprisingly, these claims are not well supported by adequate studies, the molecular mechanisms action remain largely unexplored to date. We undertook study identify characterize antitumor activity leaf extract ashwagandha. Experimental Design: Selective tumor-inhibitory (i-Extract) was identified vivo tumor formation...
Background Scopolamine is a well-known cholinergic antagonist that causes amnesia in human and animal models. Scopolamine-induced rodent models has been widely used to understand the molecular, biochemical, behavioral changes, delineate therapeutic targets of memory impairment. Although this linked decrease central neuronal activity following blockade muscarinic receptors, underlying molecular cellular mechanism(s) particularly effect on neuroplasticity remains elusive. In present study, we...
Background and Purpose Ashwagandha is a popular Ayurvedic herb used in Indian traditional home medicine. It has been assigned variety of health-promoting effects which the mechanisms remain unknown. We previously reported selective killing cancer cells by leaf extract (i-Extract) its purified component Withanone. In present study, we investigated mechanism loss-of-function screening (abrogation i-Extract induced cell killing) cellular targets gene pathways. Methodology/Principal Findings...
Background and Purpose Withanolides are naturally occurring chemical compounds. They secondary metabolites produced via oxidation of steroids structurally consist a steroid-backbone bound to lactone or its derivatives. known protect plants against herbivores have medicinal value including anti-inflammation, anti-cancer, adaptogenic anti-oxidant effects. Withaferin A (Wi-A) Withanone (Wi-N) two similar withanolides isolated from Withania somnifera, also as Ashwagandha in Indian Ayurvedic...
Sirtuins belong to a class of NAD ‐dependent deacetylases, and include seven distinct isoforms, which SIRT 7 is the least studied member. In present study, subcellular expression in primary fibroblasts undergoing senescence was evaluated by immunocytochemistry immunoblot assay. Expression nucleolar young fibroblast very prominent, decreased pre‐senescent cells, became undetectable senescent cells. Interestingly, we observed previously unreported staining for cytoplasmic fibroblasts, report...
Mortalin/mthsp70 (HSPA9) is a stress chaperone enriched in many cancers that has been implicated carcinogenesis by promoting cell proliferation and survival. In the present study, we examined clinical relevance of mortalin upregulation carcinogenesis. Consistent with high expression various human tumors lines, found overexpression increased migration invasiveness breast cancer cells. Expression analyses revealed proteins involved focal adhesion, PI3K-Akt JAK-STAT signaling, all known to play...
5-Bromodeoxyuridine was found to induce flat and enlarged cell shape, characteristics of senescent cells, senescence-associated beta-galactosidase in mammalian cells regardless type or species. In immortal human fibronectin, collagenase I, p21(wafl/sdi-1) mRNAs were immediately very strongly induced, the mortality marker mortalin changed mortal from type. Human lines lacking functional p21(wafl/sdi-1), p16(ink4a), p53 behaved similarly. The protein levels p16(ink4a) did not change uniformly,...