A5010198273- Immunotherapy and Immune Responses
- Immune Response and Inflammation
- Psoriasis: Treatment and Pathogenesis
- Dermatology and Skin Diseases
- Immune Cell Function and Interaction
- Immunodeficiency and Autoimmune Disorders
- Vibrio bacteria research studies
- Influenza Virus Research Studies
- Fibromyalgia and Chronic Fatigue Syndrome Research
- Asthma and respiratory diseases
- Respiratory viral infections research
- Monoclonal and Polyclonal Antibodies Research
- Microbial infections and disease research
- Bacteriophages and microbial interactions
- Cytokine Signaling Pathways and Interactions
- Antimicrobial Peptides and Activities
- NF-κB Signaling Pathways
- vaccines and immunoinformatics approaches
- Immune responses and vaccinations
- Inhalation and Respiratory Drug Delivery
Research Institute of Vaccines and Sera. Mechnikov of the Russian Academy of Medical Sciences
2017-2024
Psoriasis is a frequently occurring inflammatory skin disease. Fundamental research into the pathogenesis of psoriasis has significantly expanded our understanding immunology, which enabled introduction innovative and highly effective therapies. largely T-lymphocyte-mediated disease in activation innate immune cells pathogenic T leads to inflammation hyperproliferation keratinocytes. Langerhans like dendritic (DCs), play an important role system, are mainly distributed epidermis. Since human...
Imiquimod-induced model of psoriasis in mice is used for the studies psoriasis. Imiquimod administered as skin application triggers a complex multi-stage process simulating psoriatic inflammation skin, accompanied by changes production cytokines. The aim this work was comprehensive study their contents cell supernatants isolated from blood, central and peripheral lymphoid organs effect imiquimod on these parameters. Forty-six C57BL/6 were divided into two groups: (1) control (n = 22), (2)...
The aim of the study was to obtain and characterize a recombinant vaccine for immunoprophylaxis infections caused by Pseudomonas aeruginosa. A set two highly immunogenic P. aeruginosa proteins used create related vaccine. first component presented outer membrane protein F (OprF), whereas second one — atoxic exotoxin (toxoid). These antigens allowed develop inducing immune response against surface bacterial cues promote production neutralizing antibodies A, most dangerous pathogenicity...
The transcription factor NF-κB is a key in the activation of immune responses; it turn activated by pattern recognition receptors, such as TLR and NLR receptors. search for ligands activating innate immunity receptors an important scientific problem due to possibility their use adjuvants immunomodulators. In this study effect recombinant Pseudomonas aeruginosa OprF proteins toxoid (a deletion atoxic form exotoxin A) on TLR4, TLR9, NOD1, NOD2 has been investigated. was carried out using free...
Background. Experimental animal models of psoriasis helped to clarify the functions inflammatory mediators, reveal contribution innate or adaptive immune mechanisms, keratinocytes development and maintenance inflammation in psoriasis. Aim. To study subpopulation composition cells blood, skin, lymphoid organs compare two methods isolation from skin. Materials methods. The included 46 mice C57BL / 6 line, which were divided into 2 groups: experimental (n = 24) reproduce a model acute...
Aim. Study the effect of recombinant antigens P. aeruginosa on key effectors immune system. Materials and methods. Mice were immunized intraperiotoneally with 25 jig OprF 50 anatoxin sorbed aluminium hydroxide gel a 2 week interval. 7 days after last immunization spleen lymphocyte subpopulation structure was evaluated by flow cytometry. Cytokine levels in mice sera studied single at 4, 8, 24 hours 14 cytometry using FlowCytomix Mouse Thl/Th2 10 plex. Results. affect molecular-cell mechanisms...
Aim. To study the effect of vaccine preparation against Pseudomonas aeruginosa on phagocytic and bactericidal activity immunocompetent cells in mice. Materials methods. Preparation: 25 pg OprF, 50 aTox sorbed by 75 aluminum hydroxide. For immunization, recombinant protein was mixed equal weight fractions with an hydroxide gel, diluted phosphate buffered saline, for 12 hours at 4°C. The administered intraperitoneally 0.5 ml to BALB/с peripheral blood leukocytes determined from absorption...
Aim. To study the effect of OprF and aTox proteins Pseudomonas aeruginosa on cytokine profile mice dendritic cells. Materials methods. Dendritic cells (DC) were obtained from bone marrow BALB/c when cultured with 20 ng/ml recombinant GM-CSF IL-4 (Biosource, USA). P. used as inducer maturation DC. The level cytokines was determined in supernatants DC using Bio-Plex Pro™ Mouse Cytokine 23-plex Assay (BioRad, Results. Evaluation produced by demonstrates high activity mature Under influence...
Site-specific mutants as candidates for live influenza vaccines were resulted from directly introducing into the genome of pathogenic virus A/WSN/33 (H1N1) strain ts mutations derived genes encoding polymerase complex proteins some cold-adapted strains serving attenuation donor. Here we present data a comparative study examining immune system arms in mice immunized intranasally with and classical reassortant obtained by crossing Donor A/Krasnodar/101/35/59 (H2N2) bearing surface antigens...
The aim of this study was to generate dendritic cells from the bone marrow mice (DC) in vitro and assess effect virulent attenuated variants influenza virus on maturation DCs. Granulocyte-macrophage colony stimulating factor (GM-CSF) interleukin-4 (IL-4) were used combination, induce differentiation mouse (BM) mononucleocytes into On 5 th day, distinct added cell culture, additionally incubated for 2 days. morphological characteristics DCs, immunophenotype DCs expression some Toll-like...
Dendritic cells as the most active and highly specialized antigen presenting cells, play a key role in initiating immune responses. Currently, generation of medications activating dendritic for development anti-infective anticancer vaccines is relevance. Preparations microbial origin are promising to augment activity because they carry innate ligands Toll-like receptors. Such preparations include experimental protein-containing pneumococcal preparations, obtained from acetone-inactivated...