A5035887406- Influenza Virus Research Studies
- S100 Proteins and Annexins
- Cardiomyopathy and Myosin Studies
- Respiratory viral infections research
- Gold and Silver Nanoparticles Synthesis and Applications
- Muscle Physiology and Disorders
- Immunotherapy and Immune Responses
- Advanced biosensing and bioanalysis techniques
- Plasmonic and Surface Plasmon Research
- Advanced Fluorescence Microscopy Techniques
- Viral gastroenteritis research and epidemiology
- Photochemistry and Electron Transfer Studies
- Viral Infections and Immunology Research
- Advanced Biosensing Techniques and Applications
- Nanocluster Synthesis and Applications
- Biosensors and Analytical Detection
- Cardiovascular Effects of Exercise
- Extracellular vesicles in disease
- Immune Response and Inflammation
- Lipid Membrane Structure and Behavior
- Monoclonal and Polyclonal Antibodies Research
- Cystic Fibrosis Research Advances
- Neonatal Respiratory Health Research
- Adenosine and Purinergic Signaling
- Luminescence and Fluorescent Materials
Research Institute of Vaccines and Sera. Mechnikov of the Russian Academy of Medical Sciences
2007-2022
University of North Texas Health Science Center
2007-2016
University of North Texas
2007-2016
Jan Kochanowski University
2011
Texas Christian University
2010
University of Naples Federico II
2009
Institute of Food Science
2009
Molecular Sensing (United States)
2009
Institute of Protein Biochemistry
2009
Institute of Pharmacology Russian Academy of Medical Sciences
2007
Abstract Tumor-derived exosomes are emerging mediators of tumorigenesis and tissue-specific metastasis. Proteomic profiling has identified Annexin II as one the most highly expressed proteins in exosomes; however, studies focused on biological role exosomal (exo-Anx II) still lacking. In this study, mechanistic insight was sought regarding exo-Anx its function angiogenesis breast cancer Multiple vitro vivo techniques were used to study angiogenesis. Using atomic force microscopy Western...
Both TRPC6 and reactive oxygen species (ROS) play an important role in regulating vascular function. However, their interplay has not been explored. The present study examined whether activation of smooth muscle cells (VSMCs) by ROS was a physiological mechanism for tone vasoconstrictors. In A7r5 cells, arginine vasopressin (AVP) evoked striking Ca(2+) entry response that significantly attenuated either knocking down using siRNA or inhibition NADPH oxidases with apocynin diphenyleneiodonium....
Sigma-1 receptors (σ-1rs) exert neuroprotective effects on retinal ganglion cells (RGCs) both in vivo and vitro. This receptor has unique properties through its actions several voltage-gated ligand-gated channels. The purpose of this study was to investigate the role that σ-1rs play regulating cell calcium dynamics activated L-type Voltage Gated Calcium Channels (L-type VGCCs) purified RGCs. RGCs were isolated from P3-P7 Sprague–Dawley rats by sequential immunopanning using a Thy1.1...
Abstract Studies on mice showed that chitosan as an adjuvant for H5 inactivated influenza vaccines administered intramuscularly enhances significantly antibody titers and protective efficiency not only against homologous viruses, but also drift variants. Chitosan adjuvanted induced high after a single immunization with low dose of antigen. High remained at least 6 months. vaccine stored 4°C preserves its properties 8 stimulates proliferative cytotoxic activity splenic mononuclear leukocytes...
Thin films of rhodamine 6G in titanium dioxide (Rh6G/TiO2) and silicon (Rh6G/SiO2) were synthesized using the sol–gel method. We explored two kinds matrices as hosts for (Rh6G) at different concentrations dye. The pronounced effect dye concentration on absorption fluorescence spectra well time-resolved was found. In particular, it found that aggregation guest is significantly weaker Rh6G/TiO2 nanolayer. absence an isosbestic point a silica matrix suggests formation higher order aggregates,...
In this work, we describe how to realize a new sensing platform for an easy and fast detection of analytes. particular, utilized enhanced fluorescence emission on silver island films (SIFs) coupled the total internal reflection mode (TIRF) develop assay format target Here, as example, report toxic peptides present in gliadin (Gli). Our was performed follows: (1) first captured surfaces coated with anti-Gli antibodies; (2) were then incubated fluorophore-labeled (3) signal from antibody bound...
Despite major advances in pediatric cancer research, there has been only modest progress the survival of children with high risk neuroblastoma (HRNB). The long term rates HRNB United States are still 30-50%. Due to resistance that often develops during therapy, development new effective strategies is essential improve and overcome tendency patients relapse subsequent initial treatment. Current chemotherapy regimens also have a serious limitation due off target toxicity. In present work, we...
Abstract The use of inactivated poliomyelitis vaccine is very important for eradicating poliomyelitis. However, this not available readily in underdeveloped countries due to the high cost. Adjuvants can improve immunogenicity a and reduce antigen dose required vaccination, thus lowering cost vaccine. Chitosan glutamate solution chitosan sulfate micro/nanoparticle suspension were tested as adjuvants Imovax‐inactivated poliovaccine monovalent poliovirus type 1, 2, 3 vaccines obtained by...
Cyclic interactions of myosin and actin are responsible for contraction muscle. It is not self-evident, however, that the mechanical cycle occurs during steady-state isometric where no work produced. Studying cross-bridge dynamics requires an equilibrium time-resolved method (not involving application a transient). This introduces such method, which analyzes fluctuations anisotropy few molecules in Fluorescence anisotropy, indicating orientation protomer, collected from volume attoliters...
Background Experimental data accumulated over more than a decade indicate that cross‐strain protection against influenza may be achieved by immunization with conserved proteins. At the same time, efficacy of schemes designed along these lines and involving internal proteins, mostly NP M1, has not been sufficient. Objective To test immunogenicity protective DNA vaccination combination NP, M1 NS1 genes virus. Methods The was tested in mice chickens. Mice were challenged mouse‐adapted viral...
Fluorescent nanodiamonds (FNDs) are one of the new and very promising biocompatible nanomaterials that can be used both as a fluorescence imaging agent highly versatile platform for controlled functionalization to target deliver wide spectrum therapeutic agents. Among remarkable properties excellent photostability, emission between 600-700nm, quantum yield 1 moderately long lifetimes. However low absorption cross section fluorescent (N-V)- centers limits FNDs' brightness. In this work we...
Binding of ATP to the catalytic domain myosin induces a local conformational change which is believed cause major rotation an 8.5 nm α-helix that stabilized by regulatory and essential light chains. Here we attempt follow this measuring mobility orientation fluorescent probe attached near C- or N-terminus chain 1 (LC1). Cysteine 178 wild-type LC1, Cys engineered mutant was labeled with tetramethylrhodamine exchanged into skeletal subfragment-1 (S1) striated muscle fibers. In absence ATP,...
We examined the photophysical properties of new near infrared (NIR) fluorescent label SeTau-665 on a plasmonic platform self- assembled colloidal structures (SACS) silver prepared semitransparent film. A immunoassay was performed this and control glass slide. The fluorescence substantially change due to interactions. While average brightness increase SeTau 665 in ensemble measurements about 70-fold, enhancements up four-hundred times were observed certain “hot spots” for single molecule...