A5060273867- Influenza Virus Research Studies
- Viral gastroenteritis research and epidemiology
- Animal Virus Infections Studies
- Respiratory viral infections research
- RNA and protein synthesis mechanisms
- Immune Cell Function and Interaction
- Virology and Viral Diseases
- RNA Interference and Gene Delivery
- Animal Disease Management and Epidemiology
- Viral Infections and Vectors
- Poxvirus research and outbreaks
- Immunotherapy and Immune Responses
- Herpesvirus Infections and Treatments
- interferon and immune responses
- RNA modifications and cancer
- Immune Response and Inflammation
- Viral Infections and Immunology Research
Research Institute of Vaccines and Sera. Mechnikov of the Russian Academy of Medical Sciences
2017-2024
In August 2022 on a backyard farm in the Moscow region of Russia, mortality was observed among chickens, and all 45 birds particular died or were slaughtered after onset symptoms within few days. Paramyxovirus isolated from diseased birds. Based nucleotide sequences F NP gene fragments, it determined that virus belonged to subgenotype VII.1 AAvV-1 class II. The cleavage site 109SGGRRQKRFIG119 T 546 555 position typical for velogenic type. genetically closest NDV isolates found Iran. mean...
In August 2022, in the Moscow region, Russia, on a backyard farm, mortality was observed at chickens and all 45 birds of this farm died or were slaughtered after onset symptoms within few days. Paramyxovirus isolated from diseased birds. Based nucleotide sequences F NP gene fragments, belonging virus to subgenotype VII.1 AAvV-1 class II established. The cleavage site 109SGGRRQKRFIG119 T 546 555 position gene, typical for velogenic type. genetically closest NDV isolates found Iran. mean time...
Background: Influenza is one of the most common infectious diseases, which affect lower respiratory tract, and can lead to serious complications including death. It known that currently available therapeutic agents vaccines do not provide 100% protection against influenza viruses. The development drugs based on RNA interference mechanism in context this problem a promising area. This paper aims assess effect FLT4, Nup98, Nup205 cellular gene knockdown reproduction A virus human lung cell...
Objectives. To evaluate the effect of cellular genes FLT4, Nup98, and Nup205 on reproduction influenza A virus in A549 human lung cancer cell line. Methods. The work was carried out using equipment center for collective use I.I. Mechnikov Research Institute Vaccines Sera (Russia). virus-containing fluid collected within three days from moment transfection infection intensity viral assessed by titration hemagglutination reaction. RNA concentration determined real-time reverse-transcription...
Abstract Influenza A virus is a serious human pathogen that assembles enveloped virions on the plasma membrane of host cell. The pleiomorphic morphology influenza virus, represented by spherical, elongated, or filamentous particles, important for spread in nature. Using fixative protocols sample preparation and negative staining electron microscopy, we found recombinant A/WSN/33 (H1N1) (rWSN) strain considered to be strictly may produce particles when amplified allantoic cavity chicken...
Influenza is a widespread respiratory infection, accompanied by damage to the lower tract of person, and can lead severe complications, up fatal outcome. Currently existing therapeutic agents vaccines do not provide fully effective protection against influenza viruses. The development creation drugs based on mechanism RNA interference in context this problem promising direction. aim study select experimentally substantiate cellular target genes for miRNAs whose knockdown suppresses viral...
The review based on literature data and the authors' own research substantiates necessity of immunoprophylaxis respiratory syncytial virus infection (RSVI) in children at risk using palivizumab. main current epidemiology RSVI, its consequences, burden globally Russian Federation are reflected. groups severe RSVI considered as indications for listed, peculiarities course them given. experience implementing program Moscow prerequisites year-round immunization presented. formation use clinical...
Introduction. Varicella-Zoster virus (VZV) is a highly contagious alpha-herpes virus. The diagnosis of chickenpox remains difficult task especially in cases breakthrough chickenpox, so the development reliable laboratory tests necessary. simplest and most sensitive serological test for detecting antibodies human animal sera passive hemagglutination reaction (PHAR). aim. To develop simple, specific detection to VZV blood sera. Materials methods. Human cell cultures; various strains VZV;...
Введение. Ранее было показано, что полимеразные белки PB1 и PB2 определяют холодоадаптированный фенотип вируса гриппа А/Краснодар/101/35/59 (H2N2).
 Цель работы. Создать репортерные конструкции определить активность вирусной полимеразы при 33 37 °С методом минигенома.
 Материалы методы. Совместная трансфекция клеток Cos-1 плазмидами pHW2000, экспрессирующими PB1, PB2, PA, NP (минигеном) репортерную конструкцию.
 Результаты. На основе сегмента 8 созданы две конструкции, которые...
The avian influenza virus (AIV) has a great pandemic potential both in animals, and human population. For effective struggle against this virus, it is important to study the immune response AIV, and, particular, features of T cell immunity. At period early response, when activation adaptive immunity factors not yet occurred, natural killers, which have cytotoxic immunoregulatory functions, are known play key role fight virus. In study, we investigated interaction between killers helpers...
Aim. Study of changes in the phenotypic characteristics virulent A/WSN/33 (H1N1) strain influenza A virus under influence inclusion site-specific mutations PB1-gene this strain.Materials and methods. Using a two-step polymerase reaction PB1 gene A/ WSN/33 were included ts taken from genome attenuated CA donors-strains: A/Ann Arbor/6/60 (H2N2), A/Leningrad 134/17/57 (H2N2) Krasnodar/101/35/59. Ts-phenotype, att-phenotype, immunogenicity, as well weight loss mice infected with these mutants...
Site-specific mutants as candidates for live influenza vaccines were resulted from directly introducing into the genome of pathogenic virus A/WSN/33 (H1N1) strain ts mutations derived genes encoding polymerase complex proteins some cold-adapted strains serving attenuation donor. Here we present data a comparative study examining immune system arms in mice immunized intranasally with and classical reassortant obtained by crossing Donor A/Krasnodar/101/35/59 (H2N2) bearing surface antigens...
The aim of this study was to generate dendritic cells from the bone marrow mice (DC) in vitro and assess effect virulent attenuated variants influenza virus on maturation DCs. Granulocyte-macrophage colony stimulating factor (GM-CSF) interleukin-4 (IL-4) were used combination, induce differentiation mouse (BM) mononucleocytes into On 5 th day, distinct added cell culture, additionally incubated for 2 days. morphological characteristics DCs, immunophenotype DCs expression some Toll-like...
Influenza is a widespread respiratory infection, accompanied by damage to the lower tract of person, and can lead severe complications, up fatal outcome. Currently existing therapeutic agents vaccines do not provide fully effective protection against influenza viruses. The development creation drugs based on mechanism RNA interference in context this problem promising direction. aim study select experimentally substantiate cellular target genes for miRNAs whose knockdown suppresses viral...
The aim of study was to carry out comparative investigation biological properties site-specific mutants Influenza A virus and variant live cold-adapted (CA) influenza reassortant vaccine.
 Materials methods. genetic stability (SSM) the A/WSN/33 (H1N1) strain with ts (temperature sensitive)-mutations in polymerase genes studied using a stress-test MadinDarby Canine Kidney (MDCK) culture. immunogenicity U2 M26 high CA-reassortant similar surface proteins carried out. increase antibody...