A5004841964- Viral Infections and Vectors
- Vector-Borne Animal Diseases
- Influenza Virus Research Studies
- Fire effects on ecosystems
- Viral gastroenteritis research and epidemiology
- Vector-borne infectious diseases
- Mosquito-borne diseases and control
- Viral Infectious Diseases and Gene Expression in Insects
- Virus-based gene therapy research
- Protein purification and stability
- Animal Virus Infections Studies
- Respiratory viral infections research
- Monoclonal and Polyclonal Antibodies Research
- Oral microbiology and periodontitis research
- Viral Infections and Immunology Research
- Viral Infections and Outbreaks Research
- Virology and Viral Diseases
- Biochemical and Structural Characterization
- Bacteriophages and microbial interactions
- Microbial Metabolic Engineering and Bioproduction
- Bird parasitology and diseases
- Multiple Sclerosis Research Studies
- Facial Nerve Paralysis Treatment and Research
- Metabolism and Genetic Disorders
- Phytochemical compounds biological activities
Chumakov Institute of Poliomyelitis and Viral Encephalitides
2012-2024
Russian Academy of Sciences
2012-2024
Institute of Biochemical Physics NM Emanuel
2018-2024
Biological E (India)
2021
Vavilov Institute of General Genetics
2020
Moscow State University of Medicine and Dentistry
2016
Moscow State Academy of Veterinary Medicine and Biotechnology named after Skryabin
2012
Saint - Petersburg State Chemical Pharmaceutical Academy
2005
An increase in the spread of antibiotic-resistant opportunistic microorganisms causes serious problems treatment purulent infections, burns, and trophic ulcers. We tested antimicrobial activity vivo three polyphenols, Resveratrol, Dihydroquercetin (Taxifolin), Dihydromyricetin (Ampelopsin) from Norway spruce bark to promote elimination Staphylococcus aureus, Pseudomonas aeruginosa, Candida albicans wounds. Purulent infection was modelled on wounds rats infected with suspensions containing...
Previously transgenic Kalanchoe pinnata plants producing an antimicrobial peptide cecropin P1 (CecP1) have been reported. Now we report biological testing K. extracts containing CecP1 as a candidate drug for treatment of wounds infected with Candida albicans. The constitutes the whole juice from leaves (not ethanol extract) sterilized nanofiltration. A microbicide activity against animal fungal pathogen in vivo was demonstrated first time. However, favorable therapeutic effect extract...
Haemorrhagic fever with renal syndrome (HFRS) is the most widespread natural-focal human disease in Russian Federation. In this study, we report virological assessment of a fatal case HFRS-PUUV (Puumala virus) Kursk Region. The infection caused severe multiorgan failure and maximum viral load was detected tissue spleen. Viral sequences were obtained from patient's autopsy material lung tissues bank voles captured region. These formed new clade PUUV phylogenetic tree, an outgroup to all known...
Avian influenza viruses (AIV) of wild ducks are known to be able sporadically infect domestic birds and spread along poultry. Regular surveillance AIV in the is needed prepare for potential outbreaks. During long-year monitoring, 46 strains were isolated from gulls mallards Moscow ponds completely sequenced. Amino acid positions that affect pathogenicity different hosts tested. The binding affinity virus receptors analogs typical mice chickens investigated. isolates did not contain...
Hepatitis E virus (HEV) is a major causative agent of acute hepatitis worldwide, prompting continuous HEV vaccine efforts. Vaccine development hampered by the lack convenient animal models susceptible to infection with different genotypes. We produced recombinant open reading frame 2 protein (pORF2; p551) genotype (GT) 3 and assessed its immunogenicity protectivity against challenge in common marmosets (Callithrix jacchus, CM).p551 consensus sequence corresponding amino acid residues 110-660...
Relevance. Lyme disease (LD) remains an important public health problem, especially in Russia, where the incidence is consistently high. To date, there still no available vaccine against LD, and prevention involves non-specific measures. Aim: to review literature summarise data on progress, approaches strategies for LD development. Conclusions. The first vaccines were developed 1990s. An OspA-based (LYMErix) was commercially early 2000s but not widely distributed. milestone development of...
Relevance. Hemorrhagic fever with renal syndrome (HFRS) and tick-borne encephalitis (TBE) are the most common natural focal diseases of viral etiology In Russia. The medical social significance these two infections is determined by extensive fociof their spread, high annual morbidity rates in population, possibility developing severe forms disease leading to permanent disability death. causative agents HFRS Russia 6 types hantaviruses Hantaan, Amur, Seoul, Puumala, Kurkino Sochi, which,...
Relevance. Hemorrhagic fever with renal syndrome (HFRS) and tick-borne encephalitis (TBE) are the most common natural focal diseases of viral etiology in Russia. The medical social significance these two infections is determined by extensive foci their spread, high annual morbidity rates, presence severe forms disease can lead to permanent disability even death. Aim. To assess current epidemiological situation HFRS TBE Russian Federation. Conclusions. Over 23 years (from 2000 2022), 164,582...
Hemorrhagic fever with renal syndrome (HFRS) holds a leading place among natural focal human diseases in Russian Federation. There is no etiotropic therapy for the disease now. The vaccine prophylaxis most effective method to control this infection. main criteria inactivated vaccines evaluation are its immunogenicity and specific activity.The study purposes were develop sensitive real-time PCR viral RNA quantification correlation between amount immunogenicity.L-segment fragments of Puumala,...
The article presents a clinical example of Guillain-Barre syndrome with predominant involvement cranial nerves, which developed after COVID-19. Comprehensive and laboratory diagnostics, including examination cerebrospinal fluid, electromyography, for possible etiological infectious agents, was carried out. A course pathogenetic therapy used in the form plasmapheresis sessions, supportive therapy. good effect obtained. To this moment, only few cases development Guillain-Barré new coronavirus...
Introduction. Flaviviruses, possessing natural neurotropicity could be used in glioblastoma therapy using attenuated strains or as a delivery system for antitumor agents an inactivated form.
 Objective. To investigate the sensitivity of and pancreatic carcinoma cell lines to vaccine yellow fever tick-borne encephalitis viruses.
 Materials methods. Cell lines: GL-6, T98G, LN-229, MIA RaCa-2 human ductal PANC-1. Viral strains: 17D virus (YF), Sofjin (TBEV). Virus concentration were...
The H7 subtype of avian influenza viruses (AIV) stands out among other AIV. circulate in ducks, poultry and equines have repeatedly caused outbreaks disease humans. laboratory strain A/chicken/Rostock/R0p/1934 (H7N1) (R0p), which was previously derived from the highly pathogenic A/FPV/Rostock/1934 (H7N1), studied this work to ascertain its biological property, genome stability virulent changing mechanism. Several virus variants were obtained by serial passages chicken lungs. After 10 through...
Introduction. Since the development of inactivated polio vaccines, different stages production process have been changed and improved. Current vaccines based on both wild attenuated strains includes several technological stages, one which is concentration virus-containing liquid, ensures poliovirus concentration, purification liquid from a significant part ballast components.Research objective to compare characteristics ultrafiltration membranes select that provide optimal value type 1...
In the production of anti-virus vaccines, replacement wild-type with attenuated strains is becoming relevant. Sabin are most suitable candidates for this in inactivated poliovirus vaccines. The main problem development methods purification viral particles preservation their stability and antigenic properties. We have compared sorbents used gel-filtration concentrates selected a chromatographic resin, Sephacryl S-300HR, which provides high yield target product its purity both during...
ВРЕМЕННАЯ ДИНАМИКА ЦИТОКИНОВ В КРОВИ ПРИ ЭКСПЕРИМЕНТАЛЬНОМ АУТОИММУННОМ ЭНЦЕФАЛОМИЕЛИТЕ У КРЫСИзучена динамика содержания цитокинов у крыс линии Dark Agouti с индуцированным экспериментальным аутоиммунным энцефаломиелитом (ЭАЭ).В экспериментальную группу включили 11 животных, которым в подушечки задних лап инъецировали гомогенат спинного мозга беспородных крыс, смешанный неполным адъювантом Фрейнда.В контрольную 7 вводили по 100 мкл физиологического раствора, смешанного Фрейнда.У животных...