A5002298433- SARS-CoV-2 and COVID-19 Research
- Viral Infections and Immunology Research
- COVID-19 Clinical Research Studies
- Viral gastroenteritis research and epidemiology
- RNA and protein synthesis mechanisms
- Endoplasmic Reticulum Stress and Disease
- Respiratory viral infections research
- COVID-19 diagnosis using AI
- SARS-CoV-2 detection and testing
- Vaccine Coverage and Hesitancy
- Protein purification and stability
- RNA regulation and disease
- Viral Infectious Diseases and Gene Expression in Insects
- Influenza Virus Research Studies
- Evolution and Genetic Dynamics
- RNA modifications and cancer
- RNA Research and Splicing
- Mosquito-borne diseases and control
- vaccines and immunoinformatics approaches
- Bacillus and Francisella bacterial research
- Plant Virus Research Studies
- Viral Infections and Vectors
- COVID-19 Impact on Reproduction
- Animal Virus Infections Studies
- Vector-borne infectious diseases
Chumakov Institute of Poliomyelitis and Viral Encephalitides
2015-2025
Russian Academy of Sciences
2019-2025
Sechenov University
2020-2025
Centre for Research and Development
2022
Ministry of Health of the Russian Federation
2022
The unprecedented in recent history global COVID-19 pandemic urged the implementation of all existing vaccine platforms to ensure availability vaccines against every country world. Despite multitude high-quality papers describing clinical trials different products, basic detailed data on general toxicity, reproductive immunogenicity, protective efficacy and durability immune response animal models are scarce. Here, we developed a β-propiolactone-inactivated whole virion CoviVac assessed its...
ObjectivesThe outbreak of coronavirus disease 2019 (COVID-19) started in December China and then spread worldwide over the following months, involving 188 countries. The objective this study was to determine molecular epidemiology COVID-19 Russia.MethodsIn study, two severe acute respiratory syndrome 2 (SARS-CoV-2) strains were isolated genetically characterized. A phylogenetic analysis all available Russian sequences performed these compared epidemiological data on incidence evaluate...
Effective response to emerging pandemic threats is complicated by the need develop specific vaccines and other medical products. The availability of broadly countermeasures that could be deployed early in significantly alter its course save countless lives. Live attenuated (LAVs) were shown induce non-specific protection against a broad spectrum off-target pathogens stimulating innate immune responses. purpose this study was evaluate effect immunization with bivalent Oral Poliovirus Vaccine...
A key contributor to the pathogenicity of viruses is their interaction with cellular defense mechanisms, including UPR (unfolded protein response) that counteracts accumulation misfolded proteins in endoplasmic reticulum (known as ER stress). One branches mediated by IRE1 (inositol-requiring enzyme 1) protein, which possesses kinase and RNase activities facilitate unconventional cytoplasmic splicing XBP1 mRNA, leading upregulation transcription factor. In this study, we demonstrate...
We present the results of a randomized, double-blind, placebo-controlled, multi-center clinical trial phase I/II tolerability, safety, and immunogenicity inactivated whole virion concentrated purified coronavirus vaccine CoviVac in volunteers aged 18-60 open comparative IIb 60 years older. The safety was assessed 400 age cohort who received two doses (n = 300) or placebo 100) 200 60+ all whom three vaccine. studied has shown good tolerability safety. No deaths, serious adverse events (AEs),...
Abstract The severe COVID‐19 pandemic drives the research toward SARS‐CoV‐2 virion structure and possible therapies against it. Here, we characterized β‐propiolactone inactivated virions using transmission electron microscopy (TEM) atomic force (AFM). We compared samples purified by two consecutive chromatographic procedures (size exclusion chromatography [SEC], followed ion‐exchange [IEC]) with ultracentrifugation. prepared SEC IEC retained more spikes on surface than ones...
Many viruses are known to trigger endoplasmic reticulum (ER) stress in host cells, which turn can develop a protective unfolded protein response (UPR). Depending on the conditions, UPR may lead either cell survival or programmed death. One of three branches involves upregulation Xbp1 transcription factor caused by unconventional cytoplasmic splicing its mRNA. This process is accomplished phosphorylated form endoribonuclease/protein kinase Ire1/ERN1. Here, we show that phosphorylation Ire1...
Since replication of RNA-viruses is generally a low-fidelity process, it would be advantageous, if specific interactions their genomic cis-elements with dedicated ligands are relatively tolerant to mutations. The specificity/promiscuity trade-off such was addressed here by investigating structural requirements the oriL (also known as clover leaf-like element), poliovirus RNA, replicative cis-element containing conserved essential tetraloop functionally interacting viral protein 3CD. sequence...
Global polio eradication requires both safe and effective vaccines, production processes. Sabin oral poliomyelitis vaccine (OPV) strains can evolve to virulent viruses result in outbreaks, conventional inactivated (Salk-IPV) includes accumulation of large stocks neurovirulent wild polioviruses. Therefore, IPV based on attenuated OPV seems a viable option. To increase the global supply affordable still not-polio free world we developed an made from strains–PoliovacSin. Clinical trials...
This study aimed to estimate the impact of COVID-19 pandemic on circulation non-SARS-CoV-2 respiratory viruses and clinical characteristics in hospitalized children.A total 226 864 children admitted Children's City Clinical Hospital with acute infection September November 2018 2020 Moscow were tested for using multiplex polymerase chain reaction (PCR) Mycoplasma pneumoniae/Chlamydia pneumoniae enzyme-linked immunosorbent assay.The detection rate was lower than 2018, 16.9% versus 37.6%. An...
ABSTRACT We present the results of a randomized, double-blind, placebo-controlled, multi-center clinical trial tolerability, safety, and immunogenicity inactivated whole virion concentrated purified coronavirus vaccine CoviVac in adult volunteers aged 18-60. Safety was assessed 398 who received two doses (n=298) or placebo (n=100). The studied has shown good tolerability safety. No deaths, serious adverse events (AE), other significant AE related to vaccination have been detected. most...
The tick-borne encephalitis virus (TBEV) is one of the most common members Orthoflavivirus genus, which comprises causative agents severe diseases in humans and animals. Due to expanding areas orthoflavivirus infection, its differential diagnosis highly demanded. Commercial test kits based on inactivated TBEV may not provide reliable differentiation between flaviviruses because serological crossover this genus. Application recombinant domains (sE dIII) Sukhar-strain protein E as antigens an...
Abstract Vaccines are proven to be an effective tool in prophylaxis of severe COVID-19, but emerging mutated SARS-CoV-2 variants constantly challenge vaccines’ protectivity. We have evaluated the ability sera from individuals vaccinated with two inactivated vaccine CoviVac and COVID-19 convalescents (May-December 2020) neutralize Delta Omicron. Four groups serum samples (CoviVac vaccinees; convalescents; mice immunized preparations based on prototype B.1.1 strain variant) were virus...
In the production of anti-virus vaccines, replacement wild-type with attenuated strains is becoming relevant. Sabin are most suitable candidates for this in inactivated poliovirus vaccines. The main problem development methods purification viral particles preservation their stability and antigenic properties. We have compared sorbents used gel-filtration concentrates selected a chromatographic resin, Sephacryl S-300HR, which provides high yield target product its purity both during...
Replication of RNA viruses is generally markedly error-prone. Nevertheless, these usually retain their identity under more or less constant conditions due to different mechanisms mutation tolerance. However, there exists only limited information on quantitative aspects the mutational tolerance distinct viral functions. To address this problem, we used here as a model interaction between replicative cis-acting element (oriL) poliovirus and its ligand (viral protein 3CD). The conserved...
Relevance . The production and quality control of any drugs are strictly regulated. In the case antiviral vaccines, requirements for their safety protective activity getting tougher every year. Vaccine manufacturers face three tasks: ensuring high immunogenicity, availability drug. During development immunobiological drugs, must demonstrate sufficient purification from technological impurities to ensure purity Technological directly depend on process expression systems used. Vero cell line...
RNA recombination is an important and one of the main mechanisms virus evolution. Two this process are currently known [2, 3]. A recombinant molecule can be formed by replicative matrix switching [1, 2]. There also increasing information about another non-replicative mechanism viral [4]. However, role cellular factors in still under investigation [2].
BACKGROUND: The emergence of new variants the SARS-CoV-2 virus at end 2020, which became a source increased risk for global public health, prompted study their molecular biological characteristics and pathogenic effects. It is known that one reasons viruses action interaction with defense mechanisms cell. Ire1 (inositol-requiring enzyme 1)-mediated splicing Xbp1 mRNA (X-box binding protein 1) protective mechanism activated in response to accumulation misfolded proteins cell, situation occurs...
BACKGROUND: SARS-CoV-2 vaccine immunogenicity is evaluated in neutralization test with live virus. It performed a biosafety level 3 zone because requires virus stage. Therefore, control laboratories should be certified for this class of work. The development technology based on enzyme-linked immunosorbent assay as an analogue the reaction makes it possible to create immunobiological product shorter time and conditions without special requirements laboratories. AIM: Development assessing by...
BACKGROUND: Despite the large-scale development of vaccines against coronavirus infection, there is still no complete information about antigenic structure SARS-CoV-2 virus particles. This article describes a method obtaining pure concentrated whole-virion sample that can be used in various studies. AIM: The goal to develop an optimal purification inactivated order obtain standard with parameters purity and antigen content sufficient for structural immunological MATERIALS AND METHODS: To we...