A5088594670- Mosquito-borne diseases and control
- Viral Infections and Vectors
- Viral Infections and Outbreaks Research
- Viral gastroenteritis research and epidemiology
- SARS-CoV-2 and COVID-19 Research
- Virus-based gene therapy research
- Cytomegalovirus and herpesvirus research
- Transgenic Plants and Applications
- SARS-CoV-2 detection and testing
- Viral Infections and Immunology Research
- Animal Virus Infections Studies
- Bacillus and Francisella bacterial research
- CAR-T cell therapy research
- Dengue and Mosquito Control Research
- Monoclonal and Polyclonal Antibodies Research
- Rabies epidemiology and control
- Yersinia bacterium, plague, ectoparasites research
- Herpesvirus Infections and Treatments
- Viral Infectious Diseases and Gene Expression in Insects
- Respiratory viral infections research
- COVID-19 Clinical Research Studies
- Insect symbiosis and bacterial influences
- Hepatitis Viruses Studies and Epidemiology
- Virology and Viral Diseases
- Biomedical and Engineering Education
Research Institute of Vaccines and Sera. Mechnikov of the Russian Academy of Medical Sciences
2017-2024
Abstract The severe COVID‐19 pandemic drives the research toward SARS‐CoV‐2 virion structure and possible therapies against it. Here, we characterized β‐propiolactone inactivated virions using transmission electron microscopy (TEM) atomic force (AFM). We compared samples purified by two consecutive chromatographic procedures (size exclusion chromatography [SEC], followed ion‐exchange [IEC]) with ultracentrifugation. prepared SEC IEC retained more spikes on surface than ones...
Dengue fever, an infectious disease that affects more than 100 million people every year, is a global health problem. Vaccination may be the most effective prevention strategy for disease. However, development of vaccines against dengue fever complicated by high risk developing antibody-dependent increase in infection. This article describes MVA-d34 vaccine virus based on safe and MVA viral vector. The DIII domains envelope protein (E) are used as antigens, antibodies these do not cause...
The purpose of study was to isolate arboviruses from mosquitoes different species in the cell culture and identify them by using molecular immunochemical techniques.Materials methods. Viruses were isolated C6/36 cultures. pathogens identified enzyme-linked immunosorbent assay (ELISA) kits for detection antigens dengue, Chikungunya, West Nile Sindbis viruses as well reverse transcription polymerase chain reaction (RT-PCR) with specific primers Sanger sequencing.Results. A total 102 belonging...
Introduction. Chikungunya virus infection is a problem for the health care system of affected regions due to lack specific prevention, as well effective antiviral drugs. The critical role cellular immunity in viral control and clearance fever has been demonstrated many studies. Therefore, stimulation not only humoral but also undeniable importance when assessing effectiveness potential vaccine prevention this infection. aim present study was investigate formation protective after...
INTRODUCTION . Chikungunya virus (CHIKV) genotyping involves sequencing fractions of genes encoding E1, E2, and nsP1 proteins or the entire genome virus. Available reagent kits polymerase chain reaction protocols cannot be used for CHIKV genotyping, nucleic acid requires expensive equipment materials, which are not always available. Therefore, it seems promising to use a simpler more cost-effective restriction fragment length polymorphism (RFLP) method, has previously been genotyping. AIM....
INTRODUCTION. The completeness of virus inactivation and the identity vaccine strain are essential parameters for safety quality inactivated vaccines, which should be controlled during development production. Currently, most promising control methods vaccines molecular genetic methods, provide rapid results with high sensitivity specificity. AIM . aim this study was a real-time quantitative polymerase chain reaction (qPCR) method an integrated cell culture (ICC-qPCR) to assess inactivation,...
Rabies is an acute viral disease caused by a virus of the Rhabdoviridae family Lyssavirus genus, which affects central nervous system and characterised absolute mortality. Vaccination only way to prevent in humans. One products used for vaccination cultural concentrated purified inactivated dry rabies vaccine produced Federal State Budgetary Institution Science “Chumakov Scientific Center Research Development Immune-and-Biological Products Russian Academy Sciences” (hereinafter—Chumakov...
Purpose – To study the prevalence of antibodies to hepatitis E virus (AB-HEV) among population Kyrgyzstan. Materials and methods: Blood sera samples healthy people patients from hospitals Kyrgyzstan were examined by enzyme immunoassay method using tests «DS-ELISA-Anti-HEV-IgG» «DS-ELISA-ANTI-HEVIgM» (RPC Diagnostic Systems, Russia). Results: AB-HEV detected in 482 2279 studied (21.1%). The greatest number seropositive specimens found Osh Batken regions Bishkek city. Most often, persons age 1...
More than 60 recombinant monoclonal antibodies (mAbs) have been developed for the treatment of various diseases in last 20 years. About 30 antibody preparations are approved use therapy, including large group drugs against cancer. In addition, mAbs used transplantation, cardiovascular, autoimmune and, rare cases, infectious diseases. Despite fact that tens millions people die every year from viral diseases, only one drug based on prevention RSV children is currently allowed. This review...
Aim. In this study we aimed to design the universal genetic construction expressing light and heavy chains of a chimeric antibody, develop methodological approaches for production antibodies with defined specificity using monoclonal diphtheria toxin (DT) DT-17 in CHO cells as an example evaluate their immunochemical effector properties. Materials methods. Variable region genes mouse were obtained by PCR method cloned into pCI-neo plasmid vector. The S V DT -17neo «supervector» containing...
Aim. In this study we aimed to develop the methodology change antigen specificity of chimeric antibodies by replacing variable region genes in previously designed universal plasmid constructions pLK DT-17 and pHG encoding antibody against diphtheria toxin (DT) binding another DT epitope — DT-22. Materials methods. The light heavy chain regions mouse anti-DT DT-22 were amplified from hybridoma producing monoclonal reverse transcription PCR Genetic engineering methods used replace recombinant...
The severe consequences and high mortality of COVID-19 prompted the development a wide range preventive vaccines. first vaccines to be tested were developed in China formulated as inactivated SARS-CoV-2 adsorbed on aluminium hydroxide. One quality indicators for is degree adsorption, which can used control content not only non-adsorbed antigen, but also specific antigen one dose vaccine. aim study was investigate possibility desorbing from measuring its concentration using BioScan-SARS-CoV-2...
Introduction. The habitat of mosquitoes belonging to the genera Aedes spp., Culex Culiseta spp. is in South and Central America, including Nicaragua. Monitoring spread mosquito vectors assessment infection with arboviruses can provide information on possible occurrence new diseases or an increase reported cases, changes infectivity viruses for humans due pathogen transmitters.
 purpose this study was isolation identification Flavivirus Alphavirus from A. albopictus, aegypti, captured...
Scientific relevance . To date, no specific antivirals have been approved to treat and prevent Chikungunya fever, its complications, sequelae. Therefore, the development of therapeutic preventive medicinal products against virus (CHIKV), including interferon inducers, is gaining relevance. Aim The authors aimed study effectiveness prophylactic administration an inducer CHIKV in vitro model. Materials methods used two cell lines (Vero А549), a strain (Nika2021), interferon-inducing product...
Relevance . The introduction of Varicella vaccine prophylaxis explains the need to develop a methodology for monitoring vaccination effectiveness and intensity population immunity. This problem can be solved using quantitative immunoassay methods. Aim Development an enzyme-linked immunosorbent assay concentration class G immunoglobulins (AB) zoster virus (VZV) determining assessing its functional characteristics diagnostic efficiency. Materials methods Recombinant antigen GE VZV. WHO...