A5041098192- Hepatitis Viruses Studies and Epidemiology
- Herpesvirus Infections and Treatments
- Hepatitis B Virus Studies
- Viral gastroenteritis research and epidemiology
- Fluid Dynamics and Turbulent Flows
- Virus-based gene therapy research
- Nanofluid Flow and Heat Transfer
- Cytomegalovirus and herpesvirus research
- Viral Infectious Diseases and Gene Expression in Insects
- Thyroid Disorders and Treatments
- Granular flow and fluidized beds
- Atmospheric aerosols and clouds
- Immunotherapy and Immune Responses
- Heat Transfer Mechanisms
- Animal Virus Infections Studies
- Arctic and Antarctic ice dynamics
- Global Health Workforce Issues
- Icing and De-icing Technologies
- Heat Transfer and Boiling Studies
- Monoclonal and Polyclonal Antibodies Research
- Toxin Mechanisms and Immunotoxins
- Polyomavirus and related diseases
- Virology and Viral Diseases
- Particle Dynamics in Fluid Flows
- Winter Sports Injuries and Performance
Research Institute of Vaccines and Sera. Mechnikov of the Russian Academy of Medical Sciences
2017-2022
National Research Tomsk State University
1990-1992
The oncolytic potential of the attenuated mumps virus (MV) vaccine strain Leningrad-3 (L-3) was evaluated in a panel four human metastatic melanoma cell lines. lines were shown to be susceptible and permissive MV infection. Efficient replication led death cells, but effect differed among Possible mechanisms mediating selectivity L-3 towards explored. Replicative activity found depend on expression pattern type I interferon genes. None showed induction total spectrum genes required inhibit...
We made recombinant antigen GE containing fragment of VZV glycoprotein E (Gly48 - Glu135) fused to E. coli beta-galactosidase and confirmed its specificity by Western blotting competitive-inhibition enzyme immunoassay (EIA) in comparison with commercial analogues natural viral antigens. showed interaction protein IgG antibodies from rabbits immunized vaccine strain. also specifically reacted ELISA 66% sera zoster patients 35% control groups including other herpes viruses, healthy donors,...
Clinical picture of diseases caused by VZV in immunocompromised patients is often differed from healthy people and has no visible skin damage. Diagnostics such cases difficult but it important to find out real reason health deterioration for assignment the treatment. That explains usefulness molecular methods diagnostics VZV-infection. So goal this study was checking based on detection viral DNA clinical samples real-time PCR method. We used pools peripheral blood sick as materials analysis...
Aim. The development of the hepatitis E virus (HEV) genotype 1 recombinant capsid protein. Materials and methods. Escherichia coli strains, plasmid vectors, serological clinical samples, ELISA reagent kits, molecular biological, bioinformatic, biotechnological, biochemical Results. Using HEV DNA copy subgenomic RNA we made E.coli strains producing recombinabt protein, containing C-terminal fragment ORF2 protein fused to beta-galactosidase. Recombinant had been isolated from inclusion bodies...
Aim. Design аис1 construction of the hepatitis E virus (HEV) genotype 3 full-size ORF3 recombimnt polypeptide. Materials and methods. Escherichia coli strains, ptasmid vectors, serologiral biological amples, molecular biological, bioinformatic, biotechnological, biochemical serological methods.Results. RNA was isolated from pig fecal extracts collected on Belgorod farms used in RT-PCR to obtain fragment orf3 gene 3. Using A/T-cloning a recombinant plasmid obtained with insertion DNA (230 bp)...
Aim. The development of the hepatitis E virus (HEV) genotype 3 recombinant capsid protein. Materials and methods. E.coli strains, plasmid vectors, serological clinical samples, ELISA reagent kits, molecular biological, bioinformatic, biotechnological, biochemical Results. Using viruscontaining material from pigs Belgorod region (Russian Federation) we made strains producing protein, containing C-terminal viral ORF2 protein fragment fused to β-galactosidase. Recombinant had been isolated...
Aim. The development of the hepatitis E virus (HEV) genotype 1 full-size ORF3 recombinant polypeptide. Materials and methods. Escherichia coli strains, plasmid vectors, serological clinical samples, ELISA reagent kits, molecular biological, bioinformatic, biotechnological, biochemical Results. HEV RNA had been isolated from samples collected in Kyrgyzstan. DNA copy subgenomic cloned used for further E.coli strains producing protein fused to beta-galactosidase. Codons optimization method was...
The results of the innovative pilot project for kit development hepatitis E serum-diagnostic assays Interstate Program Innovative Cooperation CIS member states period up to 2020 are presented. was carried out by I.I. Mechnikov Federal State Medical Research Institute Vaccines and Sera in association with National Public Health Ministry Kyrgyz Republic Belarusian medical veterinary research institutions. As a result recombinant antigens prevalent countries HEV genotypes 1 3 were developed,...
Relevance . The introduction of Varicella vaccine prophylaxis explains the need to develop a methodology for monitoring vaccination effectiveness and intensity population immunity. This problem can be solved using quantitative immunoassay methods. Aim Development an enzyme-linked immunosorbent assay concentration class G immunoglobulins (AB) zoster virus (VZV) determining assessing its functional characteristics diagnostic efficiency. Materials methods Recombinant antigen GE VZV. WHO...