A5027561575- Virology and Viral Diseases
- Virus-based gene therapy research
- Herpesvirus Infections and Treatments
- Monoclonal and Polyclonal Antibodies Research
- Rabies epidemiology and control
- Poxvirus research and outbreaks
- Influenza Virus Research Studies
- Respiratory viral infections research
- Animal Virus Infections Studies
- SARS-CoV-2 and COVID-19 Research
- Viral gastroenteritis research and epidemiology
- Viral Infectious Diseases and Gene Expression in Insects
- Cytomegalovirus and herpesvirus research
- Microbial infections and disease research
- interferon and immune responses
- Immunodeficiency and Autoimmune Disorders
- Animal Genetics and Reproduction
- Viral Infections and Immunology Research
- Pediatric health and respiratory diseases
- Bacillus and Francisella bacterial research
- Bacteriophages and microbial interactions
- T-cell and Retrovirus Studies
- Biochemical effects in animals
- Molecular Biology Techniques and Applications
- Arctic and Antarctic ice dynamics
Research Institute of Vaccines and Sera. Mechnikov of the Russian Academy of Medical Sciences
2014-2024
Introduction. Influenza is an acute respiratory viral infectious disease caused by the influenza viruses. Current preventive and therapeutic approaches are of great anti-epidemic importance, but there a number problems, such as rapid emergence resistant strains, lack cross-immunity effectiveness vaccines. One to development anti-influenza agents use RNA interference small interfering RNAs complementary mRNA target cellular genes. Aim ‒ evaluate prophylactic effect siRNAs directed genes NXF1,...
Introduction. The emergence of new epidemiologically significant variants SARS-CoV-2 has shifted emphasis to development a live vaccine, which would be able provide protection against wide range antigenic the virus. aim study was obtain attenuated through cold adaptation and their biological characterization.Materials methods. Dubrovka laboratory strain its were cultured on Vero Calu-3 cells. virus quantification performed by titration in cells real-time reverse transcription-polymerase...
Introduction. Influenza is one of the most pressing global health problems. Despite wide range available anti-influenza drugs, viral drug resistance an increasing concern and requires search for new approaches to overcome it. A promising solution development drugs with action that based on inhibition activity cellular genes through RNA interference.
 Aim. Evaluation in vivo preventive potential miRNAs directed FLT4, Nup98 Nup205 against influenza infection.
 Materials methods. The...
Abstract. The rapid culture method for the indication of antigens rabies virus has been improved. It allows to detect antigen within 5 hours from moment cell cultures was infected. To achieve this aim it is necessary use BHK-F, polyclonal antirabies FITC labelled immunoglobulin and direct membrane immunofluorescense. Applying twofold freshly filtrated 3% paraformaldehyde as fixing solution early proteins virus.
Clinical picture of diseases caused by VZV in immunocompromised patients is often differed from healthy people and has no visible skin damage. Diagnostics such cases difficult but it important to find out real reason health deterioration for assignment the treatment. That explains usefulness molecular methods diagnostics VZV-infection. So goal this study was checking based on detection viral DNA clinical samples real-time PCR method. We used pools peripheral blood sick as materials analysis...
Introduction. Varicella-Zoster virus (VZV) is a highly contagious alpha-herpes virus. The diagnosis of chickenpox remains difficult task especially in cases breakthrough chickenpox, so the development reliable laboratory tests necessary. simplest and most sensitive serological test for detecting antibodies human animal sera passive hemagglutination reaction (PHAR). aim. To develop simple, specific detection to VZV blood sera. Materials methods. Human cell cultures; various strains VZV;...
Abstract. The high rubella incidence rate in the Russian Federation and low registration level of congenital syndrome which is not reflect epidemic situation requiring optimization diagnostics. In present study possibility early detection virus case inoculation sensitive cell culture by nasopharyngeal washouts with following antigen highly specific monoclonal antibodies to basic structural protein E1 using direct immunofluorescent method have been shown. Using rapid cultural allows providing...
Introduction. It is known that interferon a cytokine and substantial part of the immune system necessary for antigenic challenge response full expression. Also it considered every antigen an inducer. Interferon induces antivirus via binding to specific receptors, this receptors can be revealed straight on cell membranes cells. Research objective. To evaluate inducer ability some Influenza A virus strains upon indications functional activity (capacity) alpha gamma interferons peripheral...
Introduction. The antigen complex of opportunistic pathogenic bacteria (ACOPB) has a protective effect against avian influenza viruses, herpes virus type 2, and other viruses that cause acute respiratory viral infections. In the context COVID-19 pandemic, an important task is to find out whether ACOPB SARS-CoV-2.
 purpose study was evaluate in vitro virus-inhibitory activity Dubrovka laboratory strain Materials methods. performed using Vero cell line CCL-81, human peripheral blood...
Objectives . To evaluate the dynamics of expression level IL-1β and IL-28β (IFN-λ3) genes as a result complex knockdown some cellular genes, whose products play an important role in reproduction influenza virus. Methods Following collection virus-containing liquid cell lysate within three days from moment transfection infection, intensity viral was assessed using cytopathic effect titration method. The concentration ribonucleic acid (vRNA) change were determined by real-time reverse...
Until now, it has been considered that infectivity of the varicella-zoster virus (VZV) is closely related to target cell, and newly formed not released into culture medium. It also known hard grow VZV in cell cultures, due its slow replication rate a limited range sensitive cultures. In addition, isolation depends on type used, nature clinical material, presence viable transport time. Objectives. To study production infectious extracellular various Materials methods. Eight cultures were...
Introduction. Chickenpox poses a significant public health concern due to its worldwide occurrence, substantial probability of severe clinical progression, development complications that can lead fatal outcome. Routine vaccination is the only way prevent disease. The purpose this study was assess attenuation cold-adapted (CA) candidate virus strains Varicella zoster and Herpes by using traditional new methods.Materials methods. performed on diploid cells from human embryonic lung...
Purpose: characterization of vFiraVax (the causative agent chickenpox - VZV) and vZelVax shingles HZ) vaccine strains by their ability to bind preparations brain membrane receptors SPF BALB/c mice.Materials Methods. The study was performed on cold-adapted VZV HZ developed the authors basis wild-type parental pFira virus (chickenpox agent) latent lpZel (shingles agent); vOka isolated from vaccines against infection two manufactures (United Kingdom USA); HEL-3 strain diploid cells human...
BACKGROUND: Unprecedented anti-epidemic measures and the widespread use of vaccines against COVID-19 have reduced rate hospitalization mortality from disease, but not stopped SARS-CoV-2 pandemic spread. The development live COVID-19, capable providing formation a long-term humoral cellular immune response cross-protection new variants concern, is relevant. Previously at I.I. Mechnikov Research Institute Vaccines Sera cold-adapted (ca, cold-adapted) were obtained. This work aimed to search...
Журнал для непрерывного медицинского образования врачей Новый подход к оценке иммуномодулирующей и вирус-ингибирующей активности медицинских препаратов ОРИГИНАЛЬНЫЕ ИССЛЕДОВАНИЯ1 Федеральное государственное бюджетное научное учреждение «Научноисследовательский институт вакцин сывороток имени И.И.